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Biomedical subjects

J Dobiás

Publications and source records attributed to J Dobiás.

At least 19 recordsLinked to original sources
PubMed1995

[Study of malignant melanoma using 1H-nuclear magnetic resonance spectroscopy].

1H-nuclear magnetic resonance spectroscopy was used to study malignant melanoma extract. The aim of this work was to study low molecular weight metabolites soluble in water, which can be helpful for a more detailed understanding of tumor metabolism with a view to using this knowledge for diagnosis. The authors found in a well distinguished spectrum the presence of numerous low molecular weight metabolites such as glutamate, glutamine, choline, inositol, creatine, phosphocreatine, phosphocholine, glucose, acetate, alanine, lactate. It is necessary to correlate these in-vitro findings with a study of the above mentioned metabolites in-vivo. Malignant melanoma is appropriate for this investigative and diagnostic technique because of its superficial localization. (Fig. 1, Ref. 18.)

Humans↗
PubMed1994

Cellular fatty acids analysis in the identification of lactic acid bacteria.

The main factors affecting the cellular fatty acid composition such as cultivation temperature, pH and NaCl content of cultivation medium, growth stage and the method of fat isolation are summarised. The effects of these factors on the fatty acids patterns are compared with those found after the cultivation of several strains of Lactobacillus sake and L. pentosus.

Chromatography, Gas↗
PubMed1993

[The role of platelet activating factor in the pathogenesis of asthma].

The work reviews knowledge about the function of PAF, the presupposed mediator of asthma. The PAF mediator evokes contraction of smooth muscles of respiratory passages, too in vivo as in vitro, by means of an indirect mechanism involving activated platelets which function as intermediaries. The constrictor released due to this process can be represented by histamine or a product of arachidonic acid. Mucous secretion is according to current knowledge stimulated by PAF. The PAF mediator appears to be a considerable chemotactic factor for eosinophils which by their cytotoxic effects on respiratory epithelium support the origin and development of bronchial hyperresponsiveness. PAF has an eminent effect on the growth of microvascular permeability. Antagonists of PAF inhibit some mentioned PAF effects. Their possible therapeutic utilization, excluding ketotiphen, are currently subjected to research. (Fig. 2, Ref. 17.).

Asthma↗
PubMed1991

[Hyalinosis in generalized sarcoidosis located primarily in the lungs simulating a tumor].

Almost complete fibrotization and hyalinosis of sarcoid granulomas in the lungs and pleura and longitudinal extending fibrotization of alveolitis is a finding conceivable from the formal genetic aspect, however, exceptional when found in such an advanced stage. The process of fibrotization and hyalinosis in the lungs causes an ever increasing interest in the understanding of their mechanism. Today there exists already a number of known growth mediators which are produced within the framework of the altered immune state by activated monocytes and T lymphocytes. Under experimental conditions their actual importance in the process of fibrotization can be proved. In untreated sarcoidosis we observed generalization of the process which in the course of cca 15 years changed into developed adhesive pleuritis, fibrotization of the interstitium of the lung and tracheobronchial nodes, spleen and bone marrow. Neither the process proper of sarcoidosis nor cor pulmonale were the immediate cause of death which was due to massive fused pseudolobar purulent catarrhal bronchopneumonia.

Diagnosis, Differential↗
PubMed1986

[Long-term biopsy study of chronic hepatitis].

Repeated liver biopsy was performed at 293 patients suffering from chronic hepatitis (33 cases of chronic persistent type, 260 cases with mild or mediate activity, 3 highly active cases) in the interval varying from 2 months to 11 years. Unsubstantial portobiliary fibrosis was found in addition to inflammatory changes in 49 cases with mild activity, quiet fibrosis in 4 cases, unstabilized cirrhosis in 3 cases; unchanged morphology occurred but once after 4 year monitoring. Two patients with chronic persistent hepatitis showed the same picture in repeated biopsy. Both types of chronic hepatitis were obviously different as a rule but prospective difficulties in distinguishing between them are not lacking.

Biopsy↗
PubMed1985

[Light and electron microscopy findings in alcoholic liver damage].

Large centrolobular hepatocytes and the formation of alcoholic clear cells are regarded as changes of prognostic significance in chronic alcoholism. Electron microscopically these cells reveal an increase of the endoplasmic reticulum. Special attention is paid to ultrastructural peculiarities of Mallory bodies, inflammatory changes and fibrosis.

Endoplasmic Reticulum↗
PubMed1985

[Changes in pneumocytes in fibrosing alveolitis].

Some unusual changes of proliferating type II pneumocytes were shown by electron microscopy in idiopathic fibrosing alveolitis. New pneumocytes in cubic metaplasia were loosely connected by abundant microvillous processes. These were scarce on the apical surface but numerous on the basis where they are normally lacking. In the absence of basement membrane microvillous processes communicated directly with fibrosed interalveolar septum.

Humans↗