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J Dobrowolski

Publications and source records attributed to J Dobrowolski.

11 recordsLinked to original sources
PubMed1976

T cells, B cells and intermediate forms in the newborn studied by scanning electron microscopy and phosphatase marker.

Scanning electron microscopic studies of peripheral blood lymphocytes showed that percentage of T cells was lower in four cases of premature infants born between 30th and 34th week of gestation when compared with that in four cases of term infants (18.6% and 39.9% counted from 956 and 1,379 lymphocytes, respectively). The occurence of lymphocytes with intermediate patterns of surface morphology (I cells) was noted in both groups studied. Percentages of B and I cells were higher in the premature than in the term infant (26.3% and 55.1% and 20.0% and 39.1%, respectively). Analogous tendency in the T cell occurence in the premature and the term infant was demonstrated with acid phosphatase as T cell marker.

Acid Phosphatase↗
PubMedDate not supplied

Selenium effect on the growth of carcinogenic fungi and cytotoxic action of aflatoxin B1 on lymphocyte culture and on embryonal development of Xenopus laevis.

The authors report the results of investigations on the protective effect of selenium against teratogenic and cytotoxic action of aflatoxin B1 and the inhibitory action of this element on the growth of fungi producing carcinogenic aflatoxins. Sodium selenate inhibited the growth of cultures of such fungi belonging to the class Fungi imperfecti as Aspergillus flavus, Aspergillus fumigatus, Penicillium meleagrinum and Penicillium rougolosum. Added to cell cultures of lymphocytes sodium selenate inhibited the cytotoxic action of aflatoxin B1 evidenced by inhibition of PHA-stimulated blastic transformation of these cells and reduction in the number of mitoses. Sodium selenate added to the medium in the culture of Xenopus laevis larvae poisoned with aflatoxin B1 reduced significantly the early mortality of embryos and counteracted the teratogenic action of aflatoxin B1. This action was observed, however, only in early phase of morulation. The authors discuss the importance of their observations for the studies on the role of bioelements deficiency in the environment for induction of malignant transformation.

Aflatoxins↗
PubMedDate not supplied

[Effect of calf thymus extracts on the surface structure and the lysosomal apparatus of lymphocytes in patients with chronic lymphatic leukemia].

In 6 patients with chronic lymphatic leukaemia receiving subcutaneously purified calf thymus extract a greater frequency of the following processes was observed: 1) increase in the number of smooth-surface lymphocytes (T cells) in scanning electron microscopy, 2) increased number of lymphocytes forming rosettes with uncoated sheep erythrocytes, 3) increased number of lymphocytes showing high activity of acid phosphatase in cytochemical investigations.

Acid Phosphatase↗