Biomedical subjects
Publications and source records attributed to J Dohse.
Laccase activity in Trichoderma harzianum and in our own isolate Trichoderma atroviride was correlated with the production of the green pigment in conidial spores. The laccases of the two fungal species exhibit comparable kinetic parameters, pH optima and thermal sensitivity but differed in physiological properties, such as their catalytic activity during growth.
The deuteromycete Trichoderma atroviride is able to solubilize lignite in dependence on a given carbon source for growth. When cultivated on media containing glutamate, this mold excreted a set of different enzymes with hydrolytic activity. Addition of lignite to the growth media induced the synthesis of extracellular lignite-specific esterase activity but no evidence has been provided for its direct involvement in the process of lignite solubilization. Hence, the basic capability of T. atroviride enzymes to degrade a variety of ester and ether bonds at the surface or within the bulky lignite structure was tested using coal following its direct labelling with 14C-alkyl iodide. The participation of hydrolytic and oxidative enzymes in lignite degradation was assessed by measuring the release of 14C radioactivity from selectively alkylated carboxylic and phenolic OH groups. T. atroviride cleaved both carboxylic esters using esterases and the phenolic ether bonds by using oxidative enzymes, most likely laccases.
The psychic structure of 30 patients suffering from sudden deafness and of 35 patients suffering from otosclerosis was tested by the FPI. Additionally the intelligence was tested by the MWT-B. It could be shown that deafness on one ear does not cause any significant change of personality. Patients with sudden deafness and additional organic diseases did not show any psychic changes or these changes are due to a basic disease. Patients suffering from idiopathic sudden deafness can be differentiated into two groups: the first group consists of patients with no psychic disease; these patients show a good recovering tendency from sudden deafness. The second group consists of patients with a slightly neurotic personality which is in full accordance with those of psychosomatic patients. In these case the organic therapy did not lead to recovery.