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J Domaniewski

Publications and source records attributed to J Domaniewski.

At least 19 recordsLinked to original sources
PubMed2006

Ultraviolet radiation (UV) induces reorganization of actin cytoskeleton in CHOAA8 cells.

Effect of UV radiation on actin cytoskeleton was studied in CHOAA8 cells by fluorescence and electron microscopy. UV irradiated cells showed impaired adherence, disruption of the actin filaments and stronger F-actin labeling in the center of the cell. Attached cells, especially enlarged ones showed rather weak labeling of stress fibers and bundles of F-actin in the cytoplasm, but some cells with intensive labeling of these structures were also observed. Detached cells were rounded, showed strong F-actin labeling and often had buds. At the ultrastructural level UV-irradiated cells showed segmented nuclei, bodies resembling micronuclei, dilatation of endoplasmic reticulum, swollen and disturb mitochondria. Immunogold labeling of actin at the ultrastructural level was observed in non-radiated and UV irradiated cells. Actin labeling was seen in nuclei and cytoplasm. In nuclei gold particles were localized in the area of condense chromatin. Labeling for actin was not found after control incubation. Our observations show that UV radiation promotes changes in the distribution of actin in CHOAA8 cells. The results also suggest that not only reorganization of actin but changes in organelles are involved in the process of apoptosis initiated by UV radiation.

Actins↗
PubMed2005

Effect of doxorubicin on actin reorganization in Chinese hamster ovary cells.

Exposure of Chinese hamster ovary (CHO AA8) cells to doxorubicin in doses of 75 microM resulted in reorganization of F-actin filaments and characteristic feature of apoptosis. Even increase in size of CHOAA8 cells was observed. Attached cells become more flattered and elongated than control ones. Intense labeling of F-actin around the nucleus and disrupted filaments in cytoplasm as well as stress fibres and bundles of F-actin were seen. Cells detached were rather rounded and there were not stress fibres present. In these cells the network of F-actin was weak and rather disrupted and cells with buds labelled for F-actin were observed. In section from confocal microscopy labeling of F-actin in nucleus was confirmed. Electron microscopy showed cells with multisegmented nuclei. Cells with intracellular area with small and large vacuoles and containing also electron-dense material were seen. Other cellular organelles were rather well preserve. There were margination and condensation of chromatin in nucleus. Immunogold labelling of actin was observed in cells whether or not treated with doxorubicin. Positivity for actin was localized in the nuclei and cytoplasm. In the nucleus gold particles were localized predominantly in the area of condense chromatin. Positive labeling for actin was not found after control incubation. These findings show that doxorubicin promotes changes in the distribution of actin in CHOAA8 cells and that reorganization of actin in these cells is involved in process of apoptosis.

Actins↗
PubMed1998

Electron-microscopic observation of adherence of Acinetobacter baumannii to red blood cells.

It is now recognized that Acinetobacter spp. play a significant role in the colonization and infection of patients admitted to hospitals. The virulence factors of A. baumannii remains largery unknown. In this study, the adherence of A. baumannii to several species of red blood cells was investigated. The ruthenium red staining was used for electron microscopic studies. The results obtained in electron microscopy and the hemagglutination studies suggested that the thin and long fimbriae of A. baumannii participated in adhesion of these bacteria to red blood cells.

Acinetobacter↗
PubMed1996

A comparison of three staining methods in estimation of structures on the cell wall surface of Acinetobacter junii by using electron microscope.

Two strains of Acinetobacter junii isolated from groin and nasal cavity were studied for the presence of structures on the cell wall surface. Three staining methods were used. In the first thin section were contrasted with uranyl acetate and lead citrate. In this method, only electron dense amorphous material could be seen. Using only 1% uranyl acetate, thin fimbriae were observed which in few cases were collected in bundles. Staining with ruthenium red showed bacteria with thick short and long numerous peritrichous structures. Bacteria with structures concentrated near the polar regions were seen as well. From the comparative study of three methods, two of them were found to be useful: methods with ruthenium red and with 1% uranyl acetate.

Acinetobacter↗
PubMed1995

[Pneumocystis pneumonia in light of personal observations].

During a period of 10 months, 87 children with Pneumocystis carinii pneumonia accompanied transient cellular immunity disorders and with normal humoral immunity were observed. It is suggested that change the invasiveness of the parasite has changed. The necessity of taking into consideration a pneumocystis etiology in diagnosis of respiratory tract diseases among children is pointed out.

Adolescent↗
PubMed1994

Analysis of the low molecular weight nuclear polypeptide by isoelectrofocusing of nuclear proteins first separated by gel electrophoresis in SDS.

Some of the nuclear proteins, especially those tightly bound with nucleic acids, show a tendency to aggregate and some of them are also hydrophobic. Thus, SDS-containing buffers are useful for their analysis. The method of isoelectrofocusing of proteins separated by polyacrylamide gel electrophoresis in the presence of SDS was used for the analysis of the nuclear proteins. First, SDS-polyacrylamide gel electrophoresis of total chromatin proteins was conducted. The sample of the examined nuclear proteins after SDS-gel electrophoresis was isolated as a gel slice (thus corresponding to the particular molecular weight range) and used for isoelectrofocusing. This approach has been used for the analysis of about 19 kD nuclear polypeptide, tightly bound with DNA. The isoelectric point of this polypeptide has been estimated as 5.3.

Animals↗
PubMed1991

Characterization of herpes simplex virus type 1 DNA during latent infection in mice.

Micrococcal nuclease digestion technique in combination with spot-blotting hybridization have been used for the characterization of latent Herpes simplex virus type 1 (HSV-1) DNA in mouse brainstems. Two kinds of samples were used i.e. undigested DNA from mouse brainstems and DNA after prolonged digestion (i.e. for 30 minutes) with micrococcal nuclease from the same source. Each sample contained 5 micrograms of DNA (concentration was measured after digestion with micrococcal nuclease and isolation of DNA). Two kinds of probes were used: -virus specific probe, -host cellular probe. In the performed experiments, we observed the increased susceptibility of latent HSV-1 DNA for micrococcal nuclease digestion, comparing it to host DNA, which may reflect differences in protection of latent viral DNA by nuclear proteins and also may be associated with existing limited transcription of HSV-1 genome during latency.

Animals↗
PubMed1991

Relation between ultrastructural presence of CEA using peroxidase-antiperoxidase (PAP) method and tissue and serum levels of CEA in patients with breast cancer.

Ultrastructural studies were performed to detect the presence of carcinoembryonic antigen (CEA) in breast cancer by peroxidase-antiperoxidase (PAP) method. The evidenced presence of CEA was compared with the serum and tissue concentrations. A correlation between the presence of CEA at the ultrastructural level and tissue concentration was observed but not with serum levels. These studies revealed positive immunocytochemical staining for CEA when antigen concentration was 700 ng/g tissue and the reaction was strongly positive when the concentration was greater than or equal to 2000 ng/g tissue.

Breast Neoplasms↗
PubMed1991

Ultrastructural evaluation of changes in the glomeruli occurring in association with renal carcinoma.

The studies aimed at the determination of ultrastructural changes occurring in the glomeruli in association with renal carcinoma. The material was taken from 7 patients with renal carcinoma. Electron-microscopic investigation showed changes in all 7 cases. These studies demonstrated thickening of the glomerular basement membrane with electron-dense deposits on it and irregular thickening with humps. The humps were of high electron density. In some parts beside the electron-dense deposits the less dense electron lucent patches were found within the glomerular basement membrane. The foot processes of the podocytes were obliterated. Occasionally, the podocytes penetrated into basement membrane. There was mild endothelial cells proliferation in some parts, too.

Basement Membrane↗
PubMed1989

[Ductal carcinoma of the breast. Electron microscopic study].

Electron microscopic study of the breast tumors were carried out on 67 patients. There were 7 intraductal carcinomas and 60 infiltrating ductal carcinomas of the breast. Observations were made on the morphological characteristics of neoplastic cells, myoepithelial cells and basal lamina. Cells organelles in the neoplastic cells are similar in both types of ductal carcinomas. Nuclei are predominantly large and irregular with poor dense chromatin. Often a lot of filaments are around the nuclei. Myoepithelial cells in the infiltrating ductal carcinomas are ultrastructurally the same as seen in the intraductal carcinomas, but they seems to be less numerously than in the intraductal carcinomas. In the intraductal carcinomas of the breast basal lamina is irregular and from time to time is disrupt. In the infiltrating ductal carcinomas basal lamina is usually absent, but sometimes we can find its short fragments.

Adult↗
PubMed1989

[Ultrastructural evaluation of mast cells in food allergy].

In this paper we described the fine structure changes observed in mast cells from patients with food allergy. We studied material from 7 patients with allergy for the eggs and flour. Biopsies were obtained from the gastric mucosa, duodenum and rectum after the allergic provocation. Ultrastructural examination revealed mast cells mainly in the submucosa near the blood vessels. Mast cells had ultrastructural changes characteristic of degranulation. These facts suggest that there is correlation between mast cells and food allergen.

Cell Degranulation↗
PubMed1989

[Ultrastructural study of carcinoembryonic antigen (CEA) using the peroxidase-antiperoxidase (PAP) complex technic in patients with breast cancer].

In the studied material taken from 70 patients with the mammary carcinoma was tried to discover the presence of the CEA antigen in the cells on the ultrastructural level. In studies was used peroxidase-antiperoxidase method (PAP). The product of the immunocytochemical reaction was found on the surface of cell membrane. In 42 patients (60%) positive reaction was observed and in 28 (40%) reaction with PAP complex was negative. Just in 3 cases intracytoplasmic reaction was observed.

Biomarkers, Tumor↗
PubMed1989

[Ultrastructural evaluation of carcinoembryonic antigen (CEA) using an immunocytochemical method with the peroxidase-antiperoxidase (PAP) complex technic in patients with laryngeal cancer].

Peroxidase-antiperoxidase complex method (PAP) was used to reveal the CEA antigen in the larynx carcinoma cells by means of electron microscope. Studies were carried out on the material taken from 8 patients with the larynx carcinoma. In all cases positive immunoperoxidase reaction has been observed. The product of the reaction has been found on the surface of the cells. In one case intracytoplasmic positive reaction has been noticed.

Biomarkers, Tumor↗
PubMed1987

Carcinoembryonic antigen (CEA), alphafetoprotein (AFP) alpha and beta subunits of human chorionic gonadotropin (hCG) in cerebrospinal fluid of children with acute lymphoblastic leukaemia.

The cerebrospinal fluid (CSF) and plasma levels of CEA, AFP, alpha and beta hCG were determined by radioimmunoassay in 19 children with acute lymphoblastic leukaemia (ALL). CSF in 15 patients at the onset of ALL was examined in the first week after diagnosis and subsequently every two months. In 4 other children in second complete remission of ALL, CSF was examined every two months as well. Elevated values of CEACSF were present in 1/15 patients at the onset of ALL, of AFPCSF 0/15, alpha hCGCSF in 2/15, beta hCGCSF in 2/15 cases. The elevated levels of these markers in CSF became normal in successive lumbar punctures and none of these children developed central nervous system (CNS) relapse in further follow-up. Isolated CNS relapses were diagnosed 13 times in 7 children. Elevated CEACSF levels were found in 6/13 cases (maximum, 25.0 ng/ml) and in one patient CEACSF levels correlated well with pleocytosis. Elevated AFPCSF values were present in 0/13 cases, alpha hCGCSF in 0/13 and beta hCGCSF in 3/13 patients and became normal by the next CSF examination. The determination of CEA, AFP, alpha and beta hCG in plasma did not play a role in monitoring CNS relapse in ALL patients.

Adolescent↗
PubMed1987

Carcinoembryonic antigen, alphafetoprotein and alpha and beta subunits of human chorionic gonadotropin in plasma of children with acute leukaemia.

The plasma carcinoembryonic antigen, alpha-fetoprotein and the alpha and beta subunits of human chorionic gonadotropin levels were measured by radioimmunoassay in 44 children with acute leukaemia. These markers were determined repeatedly every 3 months at different stages of the disease (at onset, in complete remission, during bone marrow and extramedullary relapse). Elevated CEA levels were present in 64% of children at the onset of acute leukaemia and during bone marrow relapse. Elevated CEA levels decreased during induction treatment and they became normal with attainment of complete remission. In 7/12 patients who developed bone marrow relapse elevated CEA levels and in 4 of them raised levels appeared 3-4 months before there had been any other evidence of relapse. In 6/13 patients with extramedullary relapse, elevated CEA levels were found. AFP, alpha and beta hCG values in different stages of the disease were elevated sporadically; they did not reflect the activity of leukaemia.

Adolescent↗