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Biomedical subjects

J Dorfman

Publications and source records attributed to J Dorfman.

14 recordsLinked to original sources
PubMed1995

Inhibitory effects of class I molecules on murine NK cells: speculations on function, specificity and self-tolerance.

This review addresses the physiological role of class I-mediated inhibition of NK cell lysis. It is suggested that several distinct activating receptors can stimulate NK lysis, all of which can be inhibited by class I molecules on the target cell. Evidence that most or all peptides that bind a class I molecule can cause inhibition is discussed, supporting a model in which NK cells detect loss of class I molecules, rather than loss of specific peptide/class I complexes. Finally, the acquisition of self-tolerance among NK cells is addressed with respect to data suggesting that autoaggressive NK cells are not deleted but rather exhibit altered characteristics which may render them unable to lyse autologous cells.

Animals↗
PubMed1994

Sublexical components in implicit memory for novel words.

Five experiments investigated the role of sublexical components in implicit memory for novel words. Priming on an implicit word judgement task occurred consistently for nonwords formed out of familiar linguistic components (morphemes and syllables) but minimally for nonwords formed out of unfamiliar pseudosyllabic components. This effect was dissociable from explicit memory and insensitive to changes in the surface features of the stimuli. Moreover, it depended on unitization of stimulus components as opposed to priming of individual components. Results are interpreted in terms of the activation and integration of prior linguistic knowledge and as evidence against the role of new (perceptual or episodic) representations in implicit memory for new information.

Humans↗
PubMed1994

Implicit and explicit forgetting: when is gist remembered?

Recognition (YES/NO) and stem completion (cued: complete with a word from the list; and uncued: complete with the first word that comes to mind) were tested following either semantic or non-semantic processing of a categorized input list. Item/instance information was tested by contrasting target items from the input list with new items that were categorically related to them; gist/categorical information was tested by comparing target items semantically related to the input items with unrelated new items. For both recognition and stem completion, regardless of initial processing condition, item information decayed rapidly over a period of one week. Gist information was maintained over the same period when initial processing was semantic but only in the cued condition for completion. These results are discussed in terms of dual process theory, which postulates activation/integration of a representation as primarily relevant to implicit item information and elaboration of a representation as mainly relevant to semantic (i.e. categorical) information.

Cues↗
PubMed1991

Three brain sodium channel alpha-subunit genes are clustered on the proximal segment of mouse chromosome 2.

We have used long-range physical mapping and restriction fragment length polymorphisms between two mouse species to determine the chromosomal organization and location of the genes encoding three distinct isoforms of the alpha-subunit of the brain sodium channel. Physical mapping by pulsed-field gel electrophoresis has established that Scn2a and Scn3a (genes encoding type II and type III sodium channel alpha-subunit isoforms) are physically linked and are separated by a maximum distance of 600 kb. The segregation of restriction fragment length variations in backcross progeny of a Mus musculus and Mus spretus mating indicates that Scn 1 a (gene encoding the type I sodium channel alpha subunit) and Scn2a are tightly linked and are separated by a distance of 0.7 cM. Linkage analysis in backcross and recombinant inbred (BXD and AKXD) strains of mice localized the three sodium channel genes to the proximal segment of mouse chromosome 2 and suggested the probable gene order centromere-Hc-Neb-Pmv7-Scn2a/Scn3a-Scn1a-Mpmv 14. These results indicate that the three isoforms of the brain sodium channel alpha-subunit are encoded by three distinct genes that share a common ancestral origin.

Abnormalities, Multiple↗
PubMed1990

Cloning of the H,K-ATPase beta subunit. Tissue-specific expression, chromosomal assignment, and relationship to Na,K-ATPase beta subunits.

We have isolated cDNA clones encoding the bovine and rat gastric H,K-ATPase beta subunit. A bovine abomasum lambda gt11 cDNA library was screened with a monoclonal antibody raised against the rabbit H,K-ATPase beta subunit. A single positive phage clone containing an approximately 900-base pair cDNA insert was identified as reactive with the antibody. The identity of the cDNA was established by comparing the deduced amino acid sequence with sequences of cyanogen bromide fragments of the porcine H,K-ATPase beta subunit. Polymerase chain reaction and rapid amplification of cDNA ends were used to generate a cDNA fragment encoding the carboxyl-terminal portion of the rat gastric H,K-ATPase beta subunit. A rat stomach cDNA library was screened with the polymerase chain reaction product, and several full-length beta subunit cDNA clones were identified. The open reading frame predicts a protein of 294 amino acids with a molecular weight of 33,689. The rat H,K-ATPase beta subunit shows 41% amino acid sequence identity to the rat Na,K-ATPase beta 2 subunit and shares a number of structural similarities with Na,K-ATPase beta subunit isoforms. By analyzing the segregation of restriction fragment length polymorphisms among recombinant inbred strains of mice, we localized the H,K-ATPase beta subunit gene to murine chromosome 8. Northern and Western blot analysis reveals that this gene is expressed exclusively in stomach. Our results suggest that the H,K-ATPase and Na,K-ATPase beta subunits evolved from a common ancestral gene and may play similar functional roles in enzyme activity.

Adenosine Triphosphatases↗