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Biomedical subjects

J Doroszewski

Publications and source records attributed to J Doroszewski.

At least 19 recordsLinked to original sources
PubMed1979

[Characteristics of the erythrocyte movement in the vicinity of the flow chamber wall].

In perfusion experiments it was demonstrated that in close vicinity to the lower wall of a microscopic flow chamber a fraction of red blood cells do not exhibit the typical rotational motion, but flow in a steady position with the flat surface to the wall. The velocity of these cells is significantly smaller than that of the fluid in the same region of the channel. It is suggested that the phenomenon is caused by the action of adhesion-type forces operating between the flowing cells and the channel wall.

Adhesiveness↗
PubMed1979

The passage of lymphatic leukemia L1210 cells injected into glass bead labyrinth: their flow and retention.

Leukemia L1210 cells and normal lymphocytes were injected into the glass bead packing perfused with a medium containing serum proteins in concentration sufficient to greatly reduce the surface adhesion. The passage of radioactively labeled cells introduced as a concentrated suspension, was studied by the analysis of transit-dilution curves. It was demonstrated that a fraction of cells was retained in the labyrinth; other parameters characterizing the cell flow were similar to those of the medium. The mechanism of the arrest of cells is discussed: it is related to geometrical features of the labyrinth and the hydrodynamic forces influencing the cell surface interactions. Intense washing of the glass bead column results in the release of only a minor fraction of cells which were previously arrested. When the L1210 cells which have passed through the labyrinth without being trapped are reinjected into another glass bead layer, their retention is similar to that observed after the first injection; it appears, therefore, that the L1210 cells are uniform as concerns the properties which are important for surface interactions under flow conditions.

Animals↗
PubMed1976

Influence of serum protein and medium composition on the dynamic adhesiveness of lymphocytes and erythrocytes.

The dynamic adhesiveness of lymphocytes and erythrocytes to glass was studied in two media differing in concentration of serum protein and bivalent cations (Mg2+, Ca2+). The serum protein reduced adhesion of both kinds of cells, the effect being much greater with erythrocytes. Different adhesive properties of lymphocytes and erythrocytes under dynamic conditions were also observed in the protein-free media. Erythrocytes appear to be more sensitive to the influence of serum proteins as well as to the medium composition (in the absence of serum). Some influence of the ionic strength of the medium on erythrocyte adhesion is suggested. No effect of bivalent cations on lymphocyte adhesiveness with and without protein was observed.

Animals↗