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Biomedical subjects

J Drukker

Publications and source records attributed to J Drukker.

At least 73 records · Page 4Linked to original sources
PubMed1981

The development of different regions of the spinal cord of the developing chicken embryo under normal and experimental conditions. Acid phosphatase section histochemistry combined with ultramicrochemistry.

Sciatic section ws performed in chicken embryos of 8 and 10 days of incubation. They were sacrificed 4 dys later. Acid phosphatase (AcP) was determined with ultramicrochemical methods in several regions of the grey matter of the lumbar spinal cord. The necrosis of anterior horn cells was accompanied by a strong increase in AcP activity. Furthermore, a significant rise in AcP activity in the basal part of the contralateral posterior horn was observed. This rise was not paralleled by any obvious change in th morphology of this region.

Acid Phosphatase↗
PubMed1980

The extrinsic innervation of the pelvic organs in the female rat.

The extrinsic innervation of the pelvic organs in neonatal and adult female rats is described. Mainly an in toto acetylcholinesterase method is used; moreover, acetylcholinesterase is demonstrated in sections. The hypogastric plexus is defined as the nerve plexuses caudal to the inferior mesenteric ganglia comprising the uterine cervical ganglia and various smaller ganglia. The origin of the hypogastric plexus is formed by the hypogastric nerves, bundles of nerve fibers from the lumbar and cranial part of the sacral sympathetic trunks, bundles of nerve fibers from the inferior mesenteric ganglia and the pelvic nerves. The origin of the caudal part of the hypogastric plexus is also constituted by the pudendal nerves. The uterine nerves are derived from the ovarian nerves, the uterine cervical ganglia, the hypogastric nerves and the lumbar sympathetic trunks. The nerves to the urinary bladder are derived from the ganglionated nerve plexuses in the mesometrium as well as from the uterine cervical ganglia. The vaginal nerves are derived from the uterine cervical ganglia, the ganglionated nerve plexus in the mesometrium and the pudendal nerve. The rectal nerves are derived from the ganglionated inferior mesenteric plexus, the uterine cervical ganglia and the pudendal nerves. The hypogastric plexus in the rat is in many respects more complex than is known from the literature. These anatomical data are relevant for instance to experimentators who use the pelvic autonomic plexuses in the rat as a model system for the analysis of autonomic neuronal regulation systems in mammals.

Animals↗
PubMed1979

The extrinsic innervation of the abdominal organs in the female rat.

The extrinsic innervation of the abdominal organs in neonatal and adult female rats is described. Mainly an in toto acetylcholinesterase method was used; moreover, formaldehyde-induced fluorescence and acetylcholinesterase were demonstrated in sections. The major splanchnic nerve has its origin in the ninth and tenth thoracic sympathetic trunc ganglia. In the major splanchnic nerve a suprarenal ganglion is present. Sometimes the minor splanchnic nerve, arising from the tenth thoracic ganglion, joins the distal part of the suprarenal ganglion. The left and right major splanchnic nerves join the left and right celiac ganglia in the plexus. The left celiac ganglion is always bigger than the right one. The celiac plexus and the celiac inferior mesenteric plexus are joined by the intermesenteric plexus. Para-aortic nerves, originating in the caudal part of the thoracic sympathetic truncs, also join the abdominal prevertebral plexuses. The lumbar splanchnic nerves, not symmetrical in their origin, join the prevertebral plexuses and give off branches to the abdominal organs. The suprarenal glands receive bundles of nerve fibers, sometimes ganglionated, from the suprarenal ganglion. The kidneys are innervated from the celiac plexus, the upper lumbar splanchnic nerves and the intermesenteric plexus. The ovarian nerves are derived from the celiac plexus, the intermesenteric plexus and the upper lumbar splanchnic nerves. Bundles of nerve fibers run from the suprarenal ganglion in the celiac plexus in the direction of the suspensory ligament of the ovary. In many respects this description is at variance with existing literature on the autonomic innervation in the rat. These differences with the standard literature are relevant to those workers engaged in experiments on the sympathetic innervation of abdominal viscera.

Abdomen↗
PubMed1975

An acetylcholinesterase method for in toto staining of peripheral nerves.

UNLABELLED: Stomach, small intestine, uterus, urinary bladder, vagina, mesentery, mesometrium and joint capsule of rats, gall bladder, cystic duct and bile duct of dogs and uteri of young children are stained in toto. PROCEDURE: Tissue is perfused with saline containing hyaluronidase, then pinned on a flat layer of Paraplast and fixed for 24 hr in cold sucrose formol solution. Stomach, urinary bladder and gall bladder are also fixed in toto. Rinse for 2 days in cold 0.22 M sucrose in a sodium cacodylate buffer pH 7.2. Incubate in medium consisting of 60 mM acetate-buffer pH 5.0 or pH 5.6 (for human material only), 2 mM acetylthiocholine iodide, 15 mM Na citrate, 3 mM Cu sulphate, 0.5 mM K3Fe(CN)6, 5 times 10-4 M iso-OMPA, 1% Triton X 100 at 37C. Rinse in doubly distilled water. Dehydrate in glycerine/water mixtures of increasing glycerine content. Store in glycerine or delaminate under dissecting microscope. Delaminated specimens are mounted on gelatinized object glasses, cleared in xylene and coverslipped with Malinol. Specimens stored in glycerine can be studied microscopically. Stained specimens can also be embedded in Paraplast and sections can be studied after counterstaining.

Acetylcholinesterase↗