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Biomedical subjects

J E Burgess

Publications and source records attributed to J E Burgess.

12 recordsLinked to original sources
PubMed2003

The enzymology of sludge solubilisation utilising sulphate-reducing systems: the properties of lipases.

The first stage in the degradation and recycling of particulate organic matter is the solubilisation and enhanced hydrolysis of complex polymeric organic carbon structures associated with the sulphidogenic environment. An investigation into the enzymology of these processes has shown that lipase enzyme activities were found predominantly associated with the organic particulate matter of the sewage sludge. Sonication of the sludge gave an increase in enzyme activity as the enzymes were released into the supernatant. pH and temperature optimisation studies showed optima between 6.5 and 8 and 50-60 degrees C, respectively. All the lipase enzymes from the methanogenic bioreactors indicated extensive stability for at least an hour at their respective optimum temperatures and pH; sulphidogenic lipases reflected limited stability at these temperatures and pH during this time period. Though sulphate showed inhibitory properties towards lipases both sulphide and sulphite appeared to enhance the activity of the enzymes. It is argued that these sulphur species, liberated at different times during the sulphate reduction process, disrupt the integrity of the organic particulate floc by neutralising acidic components on the surface. The release of further entrapped enzymes from the organic particulate matter result in a subsequent enhancement of hydrolysis of polymeric material.

Biodegradation, Environmental↗
PubMed2003

Withstanding shock loads in activated sludge treatment by pre-exposure to low H2S concentrations.

An activated sludge pilot plant was used to acclimatise sludge to a low dose of H2S gas. Sludge samples from different types of treatment works were compared with acclimatised and unaclimatised sludges using batch absorption tests. The effects of sludge source and acclimatisation on the ability of the sludge to withstand shock loads of 50 ml l(-1) H2S were evaluated. Sludge that had been acclimatised to 5 ml l(-1) H2S in the air supply removed 70% of a 50 ml l(-1) load in a batch reactor after three sludge ages of acclimatisation. Pre-exposure to low H2S concentrations enabled the bacterial community to maintain a sufficient sulphide-degrading population to retain acclimatisation and degrade subsequent high loads which are toxic to unacclimatised sludge. Acclimatisation to H2S is therefore mainly an effect of selection pressure on the mixed bacterial population, which suggests that the H2S removal capacity of different types of activated sludge will converge after acclimatisation, irrespective of their initial degradative abilities.

Absorption↗
PubMed2002

Dinitrogen oxide production by a mixed culture of nitrifying bacteria during ammonia shock loading and aeration failure.

A number of experiments was conducted in order to establish if N(2)O in the exhaust gas from an aerobic consortium of nitrifiers could be used as an indicator for monitoring the nitrification process. Laboratory-scale experiments with an activated sludge system showed a strong correlation between ammonia shock loads and both the concentration of N(2)O and the rate of increase of N(2)O in the exhaust gas for shock loads less than 1.60 mg ammonical nitrogen (NH(3)-N) per g total suspended solids (TSS). For greater ammonia shock loads, correlation was found between build-up of nitrite in the aeration tank and the concentration of N(2)O in the exhaust gas from the tank. When subjecting the system to aeration failure, a similar pattern was seen, with a correlation between nitrite build-up in the aeration tank and increases in the concentration of N(2)O in the exhaust gas. The results from this work suggest that the changes in N(2)O concentration in the exhaust gas from a nitrifying process may be a useful parameter for monitoring such processes.

Aerobiosis↗
PubMed2002

Dinitrogen oxide detection for process failure early warning systems.

A number of experiments were conducted in order to establish whether the concentration of N2O in the off-gas from an activated sludge pilot plant could be used as a indicator for monitoring the nitrification process and as an early indication of ammonia appearing in the plant effluent. A strong correlation was found between ammonia shock loads and the concentration of N20 in the off-gas from the aeration tank for ammonia shock loads and dissolved oxygen depletion. When subjecting the experimental setup to doses of a nitrification inhibitor (allylthiourea) a similar pattern was seen with a correlation between nitrite build up in the aeration tank and concentration increase of N2O in the off-gas from the aeration tank. The results from this work suggest the concentration and the changes in the concentration of N2O in the exhaust gas from a nitrifying process may be a useful parameter for monitoring nitrifying activated sludge processes.

Ammonia↗
PubMed2001

Nutrient addition to enhance biological treatment of greywater.

This study compares the chemical oxygen demand (COD) removal and respiration rates of a microbial population treating real and synthetic greywaters dosed with nutrient supplements. The nutrient composition of the real and synthetic greywaters was analysed and the dosing regime for nitrogen, phosphorus and a range of trace metals planned accordingly. The doses consisted of eight single additives (macronutrients and trace metals) to the control greywater and six trace metal additions to C: N : P balanced greywater. The COD removal for the control real and synthetic greywater in lab-scale activated sludge systems (0.038 and 0.286 kg COD kg MLSS(-1) d(-1), respectively) confirmed nutrient limitation and the poor degree of greywater treatment. Nutrient dosing increased the COD removal rate and oxygen uptake rate in many cases. The greatest stimulation of microbial activity was observed with zinc additions to C: N: P balanced real greywater (1.291 kg COD kg MLSS(-1) d(-1) over 30 times the control). Inhibitory effects to various extents were rare and limited mainly to the additions of metals to synthetic greywater. The dominance of chemicals effects was observed on addition of some micronutrients; notably iron and aluminium, metals on which many coagulants for use in biotreatment of other wastewaters are based. The data indicate that the impact of understanding microbial processes and the nutrients required for wastewater treatment can only serve to optimise process efficiency for the proposed treatment of greywater.

Carbon↗
PubMed2001

Simultaneous activated sludge wastewater treatment and odour control.

Lab-scale tests were used to determine the amount of H2S that can be treated using a range of different activated sludges. Static vessels were used to study the effects of different H2S concentrations (5, 25, 50 and 75 ppm). The data indicated that odour control may be carried out using certain types of sludge, but sludge type, e.g. carbonaceous, nitrifying, with or without coagulant, affects removal efficiency. The presence of the biomass resulted in greater H2S removal than the use of wet scrubbing and the adverse effects on mixed liquor were negligible. A pilot plant was used to study the removal efficiencies of activated sludge diffusion using a typical wastewater treatment plant H2S concentration and investigated the effects that the diffusion of H2S had on the process performance. Results indicated that the levels of H2S produced by other unit processes on a wastewater treatment site (approximately 5 ppm) can be treated using activated sludge diffusion without compromising the performance of the wastewater treatment process. The only effects on the activated sludge plant observed were: (1) nitrification was interrupted briefly as H2S diffusion commenced and (2) the species' diversity in the sludge decreased.

Air Pollution↗
PubMed1998

Analysis of Y listings and medical discharges of officer Cadets at RMAS from January 1994 to May 1997, with actions to prevent injuries.

During 1995 the incidence of training related injuries to cadets at RMAS increased markedly. This paper quantifies the numbers placed on the Y List and removed from training, and those medically discharged. The main identified causes were drill, weight carriage, endurance training and lack of sleep. The training programme was modified including alterations in footwear, length and timing of physical periods and a reduction in weight carriage. Following these changes there was a highly significant fall in Y Listing from January 1996 and subsequent medical discharge.

Disability Evaluation↗
PubMed1987

Recording compound action potentials from the optic nerve in man and monkeys.

Compound action potentials were recorded from the optic nerve in patients undergoing neurosurgical operations and in rhesus monkeys. The stimuli were short light flashes delivered by light-emitting diodes that were bonded to plastic contact lenses positioned on one or both eyes, and potentials were recorded simultaneously from electrodes placed on the scalp. Potentials recorded from the optic nerve in man have an initial small positive deflection, with a latency of about 45 msec, followed by a negativity with a latency of 60-70 msec. The wave form depends on the recording site on the optic nerve and, occasionally, oscillations with a frequency around 100 Hz were seen in the responses from the optic nerve. There was considerable individual variation in the shape and size of the recorded potentials, but most potentials recorded simultaneously from an electrode placed on Oz with a reference electrode on the forehead appeared as positive deflections with latencies of about 80 msec and, occasionally, with a small positivity with a latency of about 45 msec. Compound action potentials recorded from the optic nerve near the ocular globe in the rhesus monkey in response to similar light flashes appeared as negative deflections with latencies of about 17 msec. The potentials recorded at the chiasm appeared as initial positive deflections, with the latency of the earliest peak being about 35 msec, on which oscillations with frequencies of about 100-150 Hz occasionally could be seen. The recordings from electrodes placed on the scalp (Cz-Oz and Cz-shoulder) in the monkey showed a positive peak with a latency of about 65 msec.(ABSTRACT TRUNCATED AT 250 WORDS)

Action Potentials↗
PubMed1986

Neural generators of the somatosensory evoked potentials: recording from the cuneate nucleus in man and monkeys.

The neural generators of the somatosensory evoked potentials (SEPs) elicited by electrical stimulation of the median nerve were studied in man and in rhesus monkeys. Recordings from the cuneate nucleus were compared to the far-field potentials recorded from electrodes placed on the scalp. It was found that the shape of the response from the surface of the human cuneate nucleus to stimulation of the median nerve is similar to that of the response recorded more caudally in the dorsal column, i.e., an initially small positivity followed by a negative wave that is in turn followed by a slow positive wave. The beginning of the negative wave coincides in time with the N14 peak in the SEP recorded from the scalp, and its latency is 13 msec. The response from the cuneate nucleus in the rhesus monkey has a similar shape and its negative peak appears with the same latency as the positive peak in the vertex response that has a latency of 4.5 msec; the peak negativity has a latency of about 6 msec and thus coincides with P6.2 in the vertex recording. Depth recordings from the cuneate nucleus and antidromic stimulation of the dorsal column fibers in the monkey provide evidence that the early components of the response from the surface of the cuneate nucleus are generated by the dorsal column fibers that terminate in the nucleus. The results support the hypothesis that the P14 peak in the human SEP is generated by the termination of the dorsal column fibers and that the cuneate nucleus itself contributes little to the far-field potentials.

Animals↗
PubMed1981

Diagnosis and percutaneous drainage of a perinephric abscess by ultrasound and fluoroscopy.

We describe 3 patients with perinephric abscesses in whom the perinephric fluid collections were identified by ultrasonic examination. In 2 cases thin needle aspiration of these collections using sonographic guidance provided the preoperative diagnosis. Percutaneous tube drainage under ultrasound and fluoroscopy was used successfully in 1 patient whose medical condition precluded surgical drainage. We recommend that percutaneous drainage be considered in severely ill patients with perinephric abscesses.

Abscess↗