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Biomedical subjects

J E Day

Publications and source records attributed to J E Day.

8 recordsLinked to original sources
PubMed1998

Appetite changes under free-living conditions during Ramadan fasting.

During the fasting month of Ramadan, Muslims abstain from food and drink from sunrise until sunset. This change of eating pattern provides an opportunity to investigate factors controlling hunger and thirst. A group of healthy Muslims (15 men and 26 women) living in Reading, U.K. made hourly ratings of their hunger, mood and thirst, and recorded their food and drink intake on specified days before, during and after Ramadan between January and March 1996. There were no significant changes in body weight over Ramadan. Rated hunger increased substantially during the daily fast, and hunger was higher for the women than the men during the earlier days of Ramadan, whereas later, on average, fasting levels of hunger were very similar for both sexes (significant gender x day interaction,p<0. 001). During Ramadan, the men tended to spend much of their time away from home, and may therefore have largely avoided eating-related cues during the daily fast. The women, however, were frequently involved in preparing food to be eaten later after sunset, which suggests an explanation for the results based on the external cue control of hunger. That is, unreinforced exposure to food cues in this context may have led to a decrease in the capacity of these cues to stimulate hunger.

Adult↗
PubMed1988

Tissue culture of retinal glial cells.

In order to be able to investigate the properties and characteristics of glia in the retina, a monotypic culture of retinal glial cells is likely to be an important research vehicle. Several techniques are now available to produce cultures of glial cells from the retina. These methods generally result in cultures of Mueller cells rather than retinal astrocytes. Publications on glial cultures involved complex procedures for the isolation of the target cell. Recent developments have resulted in simpler procedures with the advantage that large numbers of cultures can be established quickly and easily. Glial cultures have already been used in a variety of studies, simpler methods of culture, particularly if these can be adapted for culture of human glial cells, will probably result in more extensive use of cultures to unravel the properties of retinal glia.

Animals↗
PubMed1985

The morphological features of bovine meshwork cells in vitro and their synthetic activities.

The morphological characteristics of 3rd-passage cultured bovine meshwork cells were investigated, as were some of their synthetic activities. Growing meshwork cells had the ultrastructural characteristics of metabolically active cells whereas postconfluent cells formed gap junctions at their lateral borders and were closer in their fine structure to meshwork cells in vivo. The incorporation of 3H proline and 3H glucosamine was significantly greater in growing than postconfluent cultures. The normal bovine outflow system was rich in type I collagen but type V collagen and fibronectin was also evident. The 3rd-passage cells were shown to synthesize type I collagen, type V collagen and fibronectin. However, ultrastructural studies of the extracellular matrix which was produced in vitro demonstrated the presence of small fibrils with no distinctive banding pattern, unlike the larger collagen fibrils with a 640 A banding pattern seen in vivo.

Animals↗