Fas receptor expression on asthmatic eosinophils.
Activation of the Fas receptor (Fas; CD95) on eosinophils induces apoptosis and may play a role in controlling eosinophil number in blood and tissue. To determine whether Fas expression on eosinophils is different between asthmatics and non-asthmatics, eosinophils were isolated from the peripheral blood using a three-step procedure of dextran sedimentation, ficoll hypaque separation and negative selection with anti-CD16 monoclonal antibody bound to magnetic beads. Fas expression was quantified by flow cytometry. Fas expression and the percentage of eosinophils expressing Fas were not different between asthmatics (n=9) and non-asthmatics (n=14).
Adolescent↗