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J E Hooyman

Publications and source records attributed to J E Hooyman.

6 recordsLinked to original sources
PubMed1977

Sensitive thin-layer chromatographic method for urine screening of barbiturates.

A simple and sensitive thin-layer chromatographic (TLC) method is described for the detection of barbiturates and other sedatives at therapeutic levels in the urine. The method consists of an extraction followed by TLC on a single plate in a solvent system which separates the barbiturates from most of the other drugs. The upper portion of the plate is sprayed with a new visualization reagent, N,2,6-trichloro-p-benzoquinone imine, followed by heating. The barbiturates appear as blue spots. This procedure is capable of detecting barbiturates at levels of 0.1 mg/dl in urine.

Barbiturates↗
PubMed1977

A rapid mini-thin-layer chromatographic procedure for the detection of barbiturates and common sedatives at overdose levels.

A simple, rapid thin-layer chromatographic method is presented for the detection of barbiturates in blood, serum, or urine in overdose situations. Extraction with ether is followed by evaporation and reconstitution with methanol. Part of the extract is spotted on a 2.5 multiplied by 7.5 cm thin-layer chromatographic plate which is then developed in a mixed solvent system. After drying, the plate is sprayed with n, 2, 6-trichloro-p-benzoquinone imine and heated. The presence of a barbiturate is indicated by a blue spot. Chromatography in another solvent system assists in the identification of the particular barbiturate ingested.

Barbiturates↗