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Biomedical subjects

J E Jameson

Publications and source records attributed to J E Jameson.

7 recordsLinked to original sources
PubMed1971

Inverse care law.

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Government↗
PubMed1969

Influence of various parameters on the sensitivity of precipitin tests in farmer's lung by immunodiffusion and immunoosmophoresis.

Dilutions of a standard serum were made in saline and in negative sera, and comparative tests by immunodiffusion and by immunoosmophoresis were made against one antigenic component of Micropolyspora faeni (Thermopolyspora polyspora). The endpoint dilution of the standard serum in saline and serum in wells of different sizes was ascertained in each test. The endpoint dilution was unaffected by the distance of separation (from 1.5 mm to 6 mm) between the origins of serum and antigen, provided that the tests were read at times appropriate to each separation, the greater separations requiring longer periods of development. Higher endpoint dilutions were obtained by the use of 1% Noble agar in immunoosmophoresis than with 1% Ionagar no. 2 (Oxoid). This difference was more noticeable when low osmophoresis potentials were used.

Antigens↗
PubMed1969

Precipitins with relevance to farmer's lung and aspergillosis in normal and other sera.

Immunodiffusion, immunoosmophoresis, and precipitin inhibition tests were carried out on various categories of sera with extracts of Micropolyspora faeni (Thermopolyspora polyspora), Aspergillus fumigatus, and Thermoactinomyces vulgaris. The sera were from clinical cases of farmer's lung, from symptomless farm workers, from expectant mothers, from tuberculous and from asthmatic patients, and from patients with miscellaneous lung disorders. The clinical cases of farmer's lung became divisible into two groups by serological tests for antibody to a particular soluble antigenic component of M. faeni. The suggestion is made that one of these two groups of cases may be aetiologically unrelated to M. faeni. One of six sera tested in this group reacted strongly with an extract of T. vulgaris. Antibodies to certain components of some extracts of M. faeni and of A. fumigatus were found in a high proportion of normal sera. Possible reasons for this finding have been given.

Antigens↗
PubMed1968

Rapid and sensitive precipitin test for the diagnosis of farmer's lung using immunoosmophoresis.

An agar gel precipitin test, using osmophoresis, has been devised for use with weakly reacting sera. The test was applied to various categories of sera, including sera from cases of farmer's lung which had reacted with a conventional immuno-diffusion test, and others which had not. All the conventionally sero-positive sera reacted positively in the new test as did nine of 16 cases in the sero-negative group. Concentration of sera in the latter group resulted in 11 positive tests out of a possible 12. Evidence was obtained of qualitative differences in the sera of these two groups. Unexpected reactivity with sera from cases of farmer's lung was found in a dialysate from a broth culture of T. polyspora. No reactions with this dialysate were obtained with the sera of any patients who did not have symptoms of farmer's lung.

Agar↗