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Biomedical subjects

J E Pearson

Publications and source records attributed to J E Pearson.

At least 109 records · Page 6Linked to original sources
PubMed1970

Isolation of herpesvirus from equine leukocytes: comparison with equine rhinopneumonitis virus.

An agent which possessed the properties of herpesviruses was isolated from the leukocytes of 71 out of 80 (88.7%) apparently normal Iowa horses. It was ether- and heat-sensitive, DNA type, and produced type-A intranuclear inclusion bodies in cell cultures. Electron micrographs revealed a virion of typical herpesvirus structure. Leukocyte isolate virus could be differentiated from equine rhinopneumonitis virus (ERV) by serum neutralization, by growth differences in rabbit kidney cells, and by fluorescent antibody staining. Specific neutralizing antibody against this agent was found in a pooled serum sample from normal horses and in the serums of herpesvirus carrier horses. Serum from a mare inhibited growth of both ERV and leukocyte viral isolates. Normal sheep, calf, and rabbit serum did not neutralize either virus.

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Characterization of Newcastle disease virus (avian paramyxovirus-1) isolated from pigeons.

Newcastle disease virus (avian paramyxovirus-1) was isolated from pigeons in 12 states between May 1984 and December 1985. One of the isolates was from a feral pigeon; the remainder were from privately owned pigeon lofts. Use of monoclonal antibodies showed seven of the eight isolates tested to be indistinguishable from the 1982 and 1983 Great Britain and European isolates. Clinical signs were paralysis, torticollis, tremors, incoordination, and death. Pigeons inoculated with the paramyxovirus-1 isolates intravenously or intramuscularly developed clinical disease identical to that described for natural infection; however, only one pigeon inoculated intranasally developed clinical signs. The mean death time for inoculated pigeons was 9.5 days, with a range of 4 to 25. Virus was shed for up to 20 days. Primary lesions observed on necropsy were gastroenterocolitis and pancreatic necrosis. Chickens experimentally infected by the cloacal, intranasal, or caudal thoracic air-sac route remained healthy. However, the intracerebral pathogenicity index (ICPI) in day-old chickens was similar to that observed with velogenic Newcastle disease virus isolates. Four of six isolates inoculated intravenously into 6-week-old chickens induced neurotropic disease.

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Experimental infection of rosellas (Platycercus eximius) with velogenic viscerotropic Newcastle disease virus (VVNDV).

Plaque-purified and non-plaque-purified velogenic viscerotropic Newcastle disease viruses (VVNDVs) were inoculated into golden-mantled rosellas (Platycercus eximius). VVNDV produced acute clinical disease in this species: all birds died within 6 days postexposure. There was no difference between the two inoculation groups in clinical signs. Seven tissues and five tissue swabs were collected from each of 15 birds. The VVNDV concentration in each specimen was titrated, and the concentrations were compared. The lung and trachea had the highest concentrations of virus in both the tissue suspensions and the swab suspensions. The average virus concentrations of the lung were 10(5.9) 50% embryo lethal doses (ELD50) per 0.1 ml for the tissue suspension and 10(4.9) for the swab. The average virus concentrations of the trachea were 10(5.6) ELD50 per 0.1 ml of tissue suspension and 10(4.6) for the swab.

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Failure to demonstrate viscerotropic velogenic Newcastle disease in psittacine birds in the Republic of the Philippines.

Birds on the islands of Luzon, Mindanao, and Palawan in the Republic of the Philippines were surveyed for viscerotropic velogenic Newcastle disease (VVND). Virus isolation was attempted on 728 cloacal samples from native and exotic psittacine birds, non-psittacine birds, and domestic chickens and ducks. A VVND virus isolate was obtained from a single domestic chicken that had exhibited ocular discharge and diarrhea 3 days before sampling. All other birds were negative for VVND.

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