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Biomedical subjects

J E Sánchez

Publications and source records attributed to J E Sánchez.

13 recordsLinked to original sources
PubMed2001

Adapting substrate formulas used for shiitake for production of brown Agaricus bisporus.

A pasteurized, non-composted substrate (basal mixture) consisting of oak sawdust (28%), millet (29%), rye (8%), peat (8%), alfalfa meal (4%), soybean flour (4%), wheat bran (9%), and CaCO3 (10%) was adapted from shiitake culture to produce the common cultivated mushroom (brown; portabello), Agaricus bisporus. Percentage biological efficiency (ratio of fresh mushroom harvested/oven-dry substrate weight, %BE) ranged from a low of 30.1% (when wheat straw was substituted for sawdust) to 77.1% for the basal mixture. Special, high gas-exchange bags were required to optimize mycelial growth during spawn run. Our formula may allow specialty mushroom growers to produce portabello mushrooms on a modified, pasteurized (110 degrees C for 20 min) substrate commonly used for shiitake production without the added expense of compost preparation.

Agaricales↗
PubMed2001

Medium-dose UVA1 phototherapy in localized scleroderma and its effect in CD34-positive dendritic cells.

BACKGROUND: UVA1 radiation seems to be effective in morphea. CD34+ dendritic cells are significantly decreased in lesional skin of morphea patients. OBJECTIVE: We evaluated the therapeutic effectiveness of medium-dose UVA1 phototherapy in localized scleroderma and its effect in the number of dermal CD34+ dendritic cells in skin biopsy specimens of these patients. METHOD: Patients were irradiated with UVA1 (30 J/cm(2)) 30 times. Dermal CD34+ dendritic cells were counted before and after therapy. RESULTS: There was clinical improvement after UVA1 irradiation. Dermal CD34+ dendritic cells significantly increased after UVA1 irradiation. CONCLUSION: Medium-dose UVA1 therapy is effective in the treatment of localized morphea. Effectiveness is associated with an increase in the number of CD34+ dendritic cells in the dermis.

Adult↗
PubMed2000

Susceptibility of Helicobacter pylori to mupirocin, oxazolidinones, quinupristin/dalfopristin and new quinolones.

The in vitro activities of mupirocin, quinupristin/dalfopristin, linezolid, eperezolid, sitafloxacin, clinafloxacin, moxifloxacin, amoxycillin, metronidazole and clarithromycin were tested at pH 7.4 against 57 strains of Helicobacter pylori. The most active agents (mupirocin, sitafloxacin and clinafloxacin) were also tested for activity at pH 5.4 against the same strains. Mupirocin was very active at pH 7.4 and 5.4 (MIC90 0.25 and 0.12 mg/L, respectively). Quinupristin/dalfopristin, linezolid and eperezolid had low activity (MIC90 4, 8 and 4 mg/L, respectively). Sitafloxacin (MIC90 </= 0.008 mg/L) was the most active fluoroquinolone, while clinafloxacin (MIC90 0.12 mg/L) and moxifloxacin (MIC90 2 mg/L) were least active.

4-Quinolones↗
PubMed1999

Helminthic infection and anthropometric indicators in children from a tropical slum: Ascaris reinfection after anthelmintic treatment.

We evaluated helminthic infection and anthropometric indicators of nutritional status in a group of school-age children from a slum area of Caracas, Venezuela. The proportions of children at or below the 10th percentiles for height/age and weight/age were significantly higher in those presenting helminthiasis than in those uninfected. Although this could partially reflect a codependence of both helminthic infection and undernutrition or poverty, when the children were administered regular anthelmintic treatment for a year their anthropometric values improved significantly. When they were re-evaluated 8 months after the end of anthelmintic administration, the degree of reinfection by the most common helminth, Ascaris lumbricoides, was significantly higher in the growth-retarded children. These results confirm the relationship between helminthic infection and decreased growth rates in underprivileged populations, and indicate that children at nutritional risk are more susceptible to such infections, even after a prolonged parasite-free period.

Anthelmintics↗
PubMed1997

Herpes simplex virus encephalitis in a renal transplant patient: diagnosis by polymerase chain reaction detection of HSV DNA.

A case of herpes simplex virus (HSV) encephalitis with disseminated primary HSV infection in a renal transplant patient is described. The diagnosis of the disease was achieved by nested polymerase chain reaction (PCR)-DNA in cerebrospinal fluid (CSF). Other diagnostic measures (immunoglobulin [Ig] M and virological cultures both in blood and CSF) were negative. Blood IgG gave a false-positive signal. Although ganciclovir is not the drug of choice, its concomitant administration in our patient as a prophylactic measure against CMV infection may have decreased the usual severity normally expected in this kind of primary HSV infection. The subsequent increase in ganciclovir dose to full therapeutic range, which was implemented before the diagnosis was achieved, led to the disappearance of symptoms. The detection of PCR-DNA in CSF will probably become the diagnostic method of choice. One of its great advantages, in addition to its diagnostic reliability, is that it may obviate the performance of many cerebral biopsies.

Adult↗
PubMed1997

Treatment of cyclosporin-induced gingival hyperplasia with azithromycin.

BACKGROUND: Gingival hyperplasia is a known complication of cyclosporin therapy. Although plaque control has been shown to be of benefit, gingival surgery is occasionally necessary. The aim of this study was to review the effect of a short-course therapy with azithromycin in renal transplant patients with cyclosporin-induced gingival hyperplasia. METHODS: Thirty-eight patients received 500 mg/day of azithromycin for 3 consecutive days. The degree of gingival hyperplasia was classified as: 0, no gingival overgrowth; 1, mild overgrowth; 2, moderate overgrowth, and 3, severe overgrowth. Gingival bleeding and evolution of gingival hyperplasia were determined at 0 (pretreatment), 7, 30, 90 and 180 days. Cyclosporin, serum creatinine and ALT levels were simultaneously determined on the same days. RESULTS: Seven patients were excluded, leaving a total of 31 included in the trial. Eleven had a score of 3, 17 a score of 2, and 3 a score of 1. The degree of gingival hyperplasia was unrelated to the dose and levels of cyclosporin. Gingival hyperplasia improved in all patients (P < 0.001, Friedman test). The degree of improvement was better when the degree of hyperplasia was lower. In 27 patients gingival hyperplasia remained absent 6 months later, 3 patients required a second course of treatment, and another required gingival surgery. Gingival bleeding, present in 28 patients when diagnosed, disappeared in all cases in 2.2 +/- 1.2 (1-7) days. No adverse effects were observed. Cyclosporin, serum creatinine, and ALT levels were not affected by treatment. CONCLUSIONS: Azithromycin improves cyclosporin-associated gingival hyperplasia, especially when administered early in the process.

Adult↗
PubMed1987

[Use of chigo (Campsiandra comosa Benth) seeds in human nutrition. III. Energetic value of chiga flour].

The proximate composition as well as vitamin and mineral contents of a sample of chiga (Campsiandra comosa Benth) flour was analyzed, having determined that it was essentially a carbohydrate source. The product was evaluated as source of energy, substituting 33, 67 and 100% of the corn starch of isoproteic and isocaloric feeds for growing Sprague-Dawley rats. In an experimental period of 15 days, results indicated that the substitution of one-third of the carbohydrate with chiga flour did not reduce the weight gain, food consumption, metabolizable energy or utilization of the food energy by the rats. Higher levels of the product in the diet originated a decrement in these variable values. The apparent nitrogen and energy digestibility of the diets was progressively reduced as the concentration range of chiga flour utilized was increased. The metabolizable energy value of chiga flour was 3.15 kcal/g, which indicated that approximately 78% of its gross energy (4.01 kcal/g) was metabolized.

Animals↗
PubMed1977

Sensitivity of Bacteroidaceae to fosfomycin.

The sensitivity of 116 strains of Bacteroides fragilis and 60 strains of Sphaerophorus to fosfomycin was studied. Of the 178 strains that were tested, 156 were isolated from pathological products in our department and the remainder came from the following collections: NCTC, ATCC and the Institut fur Mikrobiologie of the University of Bonn. Identification was made morphologically and biochemically in accordance with the scheme which we usually employ and which has previously been published. The sensitivities were determined by the progressive dilution method in a solid medium with a surface inoculation of the strain. The results obtained show that no matter what kind of B. fragilis was tested, none of them were sensitive to concentrations of 200 mug/ml fosfomycin. On the other hand, all strains of Sphaerophorus were sensitive to lesser concentrations than the aforementioned. The majority of these concentrations subsequently lie within therapeutic margins. The conclusion drawn from our results is that the sensitivity to fosfomycin constitutes a test for differentiating Bacteroides from Sphaerophorus and that fosfomycin is ineffective in injections caused by Bacteroides, although it is an active antibiotic against infections caused by Sphaerophorus.

Anti-Bacterial Agents↗
PubMedDate not supplied

A look in the mirror: a critical and exploratory study of public perceptions of the chiropractic profession in New Jersey.

This paper reports the results of a telephone survey of 693 respondents which was commissioned by the New Jersey Chiropractic Society. This exploratory study provides a broad-ranging and critical examination of key aspects relating to the chiropractic profession as it is practiced in New Jersey. The study concludes that chiropractic in New Jersey is a viable means of treating various disorders, but there remains much confusion and distrust among prospective and current patients as well as the threat of heightened competition from other health professionals. Chiropractic must develop clear boundaries around the number and extent of the conditions it claims to treat, and must substantiate its claims with valid clinical trials. A research-based education campaign is necessary if the discipline is to encourage nonusers to become users. The most powerful vehicle for influencing public opinion and the number of referrals is family and friends. The findings also indicate that comparisons with medical doctors can backfire, making the "chiropractic physician" (the professional label preferred by most respondents) less qualified by comparison. Rather than reject the paradoxical nature of their role as primary care physicians and specialists in spine-related disorders, chiropractors should embrace this uniqueness and establish themselves as a integral component of the health care network. Clearer boundaries, a grounding in scientific clinical research and better interprofessional relations can ensure continued growth and success in New Jersey.

Adult↗