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J F Chavez

Publications and source records attributed to J F Chavez.

3 recordsLinked to original sources
PubMed2000

Application of 16S rRNA gene sequencing to identify Bordetella hinzii as the causative agent of fatal septicemia.

We report on the first case of fatal septicemia caused by Bordetella hinzii. The causative organism exhibited a biochemical profile identical to that of Bordetella avium with three commercial identification systems (API 20E, API 20 NE, and Vitek GNI+ card). However, its cellular fatty acid profile was not typical for either B. avium or previously reported strains of B. hinzii. Presumptive identification of the patient's isolate was accomplished by traditional biochemical testing, and definitive identification was achieved by 16S rRNA gene sequence analysis. Phenotypic features useful in distinguishing B. hinzii from B. avium were production of alkali from malonate and resistance to several antimicrobial agents.

Aged↗
PubMed1976

Protein quality of some representative Latin American diets by rat bioassay.

A study of the protein quality of twelve different food-mixtures common to Latin America, was performed by rat bioassay. The individual foods were cooked and dried and then mixed in the appropriate proportions resembling the original meals. Rats of the Sprague-Dawley strain were used for the bioassays. Special emphasis was given to the methodology of the slope-ratio techniques preconized by Hegsted and his associates who have claimed that classical bioassays overestimate the nutritive value of poor quality proteins. RNV and RPV values were calculated using the slope-ratio technique, with and without use of a non protein control group. PER, NPR, relative NPR and utilizable protein were also calculated using the same data. The amino acid composition was determined and the score against the FAO/WHO 1973 pattern was compared with the bioassays. Protein value of the diets was also calculated from food table data. Agreement was good between all the procedures especially for the calculation of utilizable protein although the poorest diet containing little else but maize was somewhat overestimated in its quality by calculation from food table data. Thus, in general, estimates for protein quality of diets by standard bioassay techniques and even by amino acid scoring procedures gave values comparable to those obtained by the slope-ratio assays. The large scale re-evaluation suggested for all protein quality data prior to the slope-ratio techniques may not in fact be necessary.

Amino Acids↗