Biomedical subjects
Publications and source records attributed to J F Hamel.
Hydrodynamic phenomena in microcarrier cultures are investigated with regard to the development of improved reactor designs for large-scale operations. New concepts and theoretical models that describe new data as well as previously published data are presented.
Many industrially important proteins can now be expressed intracellularly as insoluble protein inclusion bodies. In production, large-scale centrifugation is commonly used to separate and recover the inclusion bodies. Recovery efficiency depends critically on the centrifuge feed rate, which must be optimized to minimize production costs. We have used a disc centrifuge photosedimentometer to make high-resolution measurements of the particle size distribution (PSD) of the supernatant during the production of porcine somatotropin (pST) inclusion bodies. These measurements readily monitor the breakthrough of inclusion bodies into the supernatant and allow the centrifugation operation to be optimized.