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Biomedical subjects

J F Longworth

Publications and source records attributed to J F Longworth.

13 recordsLinked to original sources
PubMed1980

Naturally occurring IgM antibodies to a small RNA insect virus in some mammalian sera in New Zealand.

Antibodies to cricket paralysis virus were demonstrated in sera from a pig, a horse, and numerous cattle in New Zealand. The reactions in immunodiffusion tests were variable, but significant and consistent reactions were obtained with these sere in virus neutralization assays using Drosophila cells. The sedimentation coefficient of the antibodies was 19S, and their activity was destroyed by treatment with 0.1 M 2-mercaptoethanol, indicating that they were of the IgM class.

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PubMed1977

A solid-phase radioimmunoassay for polyhedron protein from baculoviruses.

A solid-phase radioimmunoassay has been developed for detecting polyhedron protein of Wiseana spp. nuclear polyhedrosis virus (W.NPV) using antigen fixed to plastic microtiter wells. The direct assay using 125I-labeled rabbit immunoglobulins could detect 0.2 microng of polyhedron protein, and the indirect method using 125I-labeled sheep antirabbit immunoglobulins could detect 0.05 microng of polyhedron protein. Antibodies against W.NPV polyhedron protein could be detected using a competition assay with labeled and unlabeled antibodies, providing a method suitable for rapid and quantitative screening of sera.

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PubMed1976

A small RNA virus with a divided genome from Heteronychus arator (F.) [Coleoperai Scarabaeidae].

Some properties of a small RNA virus isolated from larvae of Heteronychus arator [Coleoptera: Scarabaeidae] are described. The virus develops in the cytoplasm of gut and fat body cells and is 30 nm in diamter. The sedimentation coefficient of virus was 137S and the buoyant density was 1-33 g/ml. The single stranded RNA was isolated as two species which sedimented at 22S and 15S, corresponding to mol. wt. of 1-0 and 0-5 X 10(6) respectively. The base ratios of the two RNA species were 22 S: A=29-0, C=24-1, G¿-8, U=24-1; 15S:A=26-4, C=23-8, G=24-7, U=25-2. There was one major polypeptide, mol. wt. 40 000 and two minor polypeptides in the virus. The virus was infective for Galleria mellonella and some other insects, but was not infective for mice. No evidence was obtained to suggest that the two RNA species were present in different particles.

Animals↗
PubMed1973

Parvoviruses.

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Animals↗