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Biomedical subjects

J F Mohn

Publications and source records attributed to J F Mohn.

At least 19 recordsLinked to original sources
PubMed1981

The inhibitory properties of group A and B non-secretor saliva.

The existence of some form of specifically active A or B blood group substance in the saliva of group A1, A2 and B non-secretors was demonstrated. Significant inhibition of agglutination results were obtained when the salivas of these non-secretors were examined in a test procedure using anti-A + B (group O) serum and group AX red cells. Native saliva specimens from group A1, A2 and B secretors and non-secretors were filtered on Sephadex G-200 columns. The eluates were tested for blood group activity by inhibition of agglutination and for relative carbohydrate and protein content. A main excluded glycoprotein, blood group-active fraction was detected in all samples of saliva examined.

ABO Blood-Group System↗
PubMed1980

The production of anti-a1 agglutinins in A-like rabbits injected with human group A1 erythrocytes.

A-like and non-A-like rabbits were injected with human group A1 erythrocytes. The rabbit sera were examined serologically for their hemagglutination patterns with human red cells of groups A1 and A2 before and after sequential adsorption with human group O, B and A2 erythrocytes. The A-like rabbits produced specific anti-A1 heteroagglutinins that resisted further adsorptions with group A2 cells. The tissues of A-like rabbits obviously do not contain antigenic configurations equivalent to those on human group A1 erythrocytes. Cross-reacting antibodies were also formed, by the non-A-like rabbits especially, as evidenced by the increased titer of agglutinins reactive with human group B red cells.

ABO Blood-Group System↗
PubMed1980

The production of anti-A1 agglutinins in A-like rabbits injected with human group a1 secretor saliva.

A-like and non-A-like rabbits were injected with human group A1 and A2 secretor saliva. The sera were then examined for their agglutinative capacity against human group A1, A2, B and O erythrocytes before and after sequential adsorption with group O, B and A2 erythrocytes. The A-like rabbits produced specific anti-A1 agglutinins when injected with group A1 saliva but not when injected with group A2 saliva. The non-A-like rabbits produced anti-A and anti-A1 agglutinins in response to the A1 antigen but only anti-A agglutinins when group A2 saliva was injected. These experiments support the interpretation that a qualitative difference exists between subgroups A1 and A2. The non-A-like rabbits also formed cross-reacting antibodies as shown by the increase in the agglutinins reactive with human group B red cells.

ABO Blood-Group System↗
PubMed1980

Heteroimmunogenicity of human salivary blood group A substance in rabbits.

Rabbits were selected on the basis of distinctly higher titers of preexisting heteroagglutinins in their normal sera for human group A1 and A2 erythrocytes than for group B and especially group O. They were injected intravenously with increasing volumes of either of two pools of human group A1 secretor saliva. Comparative titrations between the reimmunization and the 7--14-day postimmune sera clearly demonstrated that all the rabbits responded with increases in the titers of the agglutinins directed against human group A1 and group A2 red blood cells that averaged approximately 250-fold; whereas, the titers of those active against the group B cells increased but 9.2 times and of those reacting with the group O cells only by an average of 2.8 times.

ABO Blood-Group System↗
PubMed1978

Influence of various physicochemical factors on hemagglutination.

A study is made of the influence on hemagglutination of various physicochemical parameters, such as: cell shape, cell distance, extracellular colloid-osmotic pressure, degree of hydration of the cell surface, and cell zeta-potential. To a varying extent these are changed by: the addition of divers soluble polymers, the action of enzymes, the presence of various salt ions, centrifugation, agglomeration at low ionic strength, complex coacervation with cationic polymers, and the interaction with different kinds of antibodies. Among the more important parameters are cell distance and cell shape (particularly when the latter tends to spiculation), while the influence of zeta-potential appears to be of fairly minor consequence. The action of dissolved polymers is mediated through polymer bridging, increase in extracellular colloid-osmotic pressure, decrease of cell surface hydration, and change in cell shape. A peculiarity of dextran is that in addition to all of the above, it causes pronounced erythrocytic spiculation.

Antigen-Antibody Complex↗
PubMed1976

Agglutination of group Ax erythrocytes by anti-A sera (group B).

Four random examples of group Ax erythrocytes were tested with anti-A sera (group B). Microscopic examination demonstrated a high incidence of reactivity of these cells with the anti-A sera (group B) obtained after immunization with group A substance.

ABO Blood-Group System↗