Biomedical subjects

J F Schmitt

Publications and source records attributed to J F Schmitt.

7 recordsLinked to original sources

PubMed1992

A new quantitative polymerase chain reaction-high performance ion exchange liquid chromatographic method for the detection of fibroblast growth factor-beta (FGF-beta) gene amplification.

A method for the quantitative determination of fibroblast growth factor-beta (FGF-beta) genomic amplification based on the use of optimized polymerase chain reaction (PCR) procedures and high performance ion exchange (HPIEX) liquid chromatography has been developed. Co-amplification of a second genomic species permits internal standardization of the techniques during optimization of the reaction conditions, the PCR cycle number and the PCR cycle efficiency, as well as during the analytical HPIEX chromatographic determination of the PCR products. These investigations confirm the versatility of these procedures to quantitatively analyse FGF-beta gene amplification in various cells and tissues.

Base Sequence↗

PubMed1990

Subject descriptions, control groups, and research designs in published studies of language-impaired children.

We reviewed the 1983-1988 issues of six journals that frequently publish papers including specifically language-impaired (LI) subjects. A total of 92 research reports provided data for our review. The research reports included experimental studies, ex post facto studies, and intervention studies. These studies represent a broad spectrum of the theoretical and empirical foundations of knowledge regarding LI children. The analysis of the published research centered on subject descriptions and the use of control groups. A descriptive analysis of the data showed few consistent trends among the studies with respect to subject selection, subject description, and the number and types of control groups. We discuss the importance of more complete subject descriptions in studies of LI children as well as the importance of the choice of matching criteria for control groups in between-subjects designs.

Child↗

PubMed1989

Selected pragmatic features in Spanish-speaking preschool children.

We assessed Spanish-speaking preschool children for the development of seven language functions and three discourse features. Analyses consisted of spontaneous language samples averaging 136 utterances per child, for 18 subjects between 3:0 and 4:5 (years:months). Data for the frequency of occurrence and the percentage of appropriate usage showed that the preschoolers had established communicative competence for the functions and discourse features. Implications include establishing preliminary guidelines for the development of normal pragmatics in Hispanic preschoolers. We also discuss the 10-item taxonomy as a reliable and clinically useful tool with either English-speaking or Spanish-speaking children.

Central America↗

PubMed1989

Natural alteration of speaking rate: the effect on passage comprehension by listeners over 75 years of age.

The purpose of the study was to assess the comprehension performance of "old-old" listeners for naturally rate-altered passages. Fourteen men and 14 women between 75 and 84 years of age responded to comprehension questions after hearing matched passages at four different rates. The listeners' performances were significantly worse under 60% time compression than at normal rate and two rates of time expansion. Comprehension differences between the sexes were not significant. The results are compared with prior findings for old-old listeners responding to electronically altered speech stimuli (Schmitt, 1983a) and for "young-old" listeners (65-74 years of age) who also responded to naturally rate-altered passages.

Aged↗

PubMed1988

Sequence and transcriptional analysis of the vaccinia virus HindIII I fragment.

The complete sequence of the vaccinia virus HindIII I fragment, which is composed of 6,498 base pairs, encodes six complete and two incomplete open reading frames (ORFs). Computer analysis revealed an amino acid sequence homology between ORF I 4 and the large subunit of the ribonucleotide reductase complex. The two small polypeptides derived from ORFs I 2 and I 5, with molecular weights of 8,500 and 8,700, respectively, have a very high hydrophobic amino acid sequence composition. S1 analysis revealed that ORF I 4 is expressed at early stages of infection, ORFs I 1, I 2, I 5, and I 7 are expressed in the late phase of infection, and ORF I 3 is constitutively expressed. Screening a vaccinia virus genomic library revealed a large vaccinia virus insert overlapping the HindIII I and O fragments which contains a previously undetected HindIII P fragment of approximately 300 base pairs. S1 analysis revealed an early (O1) and a late (O2) start site of transcription initiation located within the HindIII O fragment.

Amino Acid Sequence↗

PubMed1986

Variable speech control vs Varispeech: does newer mean better?

Normal-hearing volunteers (N:30, aged 19-28 yrs) yielded intelligibility scores for W-22 word lists and CID sentences at 40 db re each S's SRT, and judgments on a 7-pt scale ("very pleasant"----"very unpleasant") for Sentence 3 of Fairbanks' "Rainbow Passage". Normal and time-altered speeds (60%: compression; 140%: expansion) were compared between the widely-used but obsolescent Lexicon Varispeech (VSp) unit vs the commercially available Variable Speech Control Model A-7 which incorporates a time-sampling technique similar to the VSp. With both VSp and VSC, intelligibility was nearly perfect at all three speeds, and thus both appear equally suitable for intelligibility studies, but at the 60% and 140% speeds the signal clarity was significantly preferable for the VSp system (p less than .01 in both cases), by .6 of a scale interval at 60% and 1.8 interval at 140%. Furthermore, the VSC does not operate beyond the 60-140% range, as occasionally may be desirable in research on the effects of time alteration on speech reception.

Adolescent↗

PubMed1985

Older listeners' ability to comprehend speaker-generated rate alteration of passages.

The purpose of this investigation was to determine the effect of speaker-generated (natural) rate alteration on passage comprehension performance by healthy older listeners. Fourteen men and 14 women between 65 and 74 years of age were tested. The passages were recorded at 60% time compression, 0% alteration (normal rate), and at time expansion values of 140% and 180%. Comprehension was measured by scoring the responses made by subjects to questions that were pertinent to each passage. The data were analyzed for comprehension performance differences among the four rate alteration conditions and between the men and women. The group performed significantly more poorly only at 60% time compression when compared with normal rate; there were no differences among the three slower rates. The findings are compared with prior studies of rate-altered stimuli with older listeners and are discussed relative to potential differences in the responses of older persons to electronically altered as opposed to naturally altered speech rate.

Aged↗