Biomedical subjects

J F Vago

Publications and source records attributed to J F Vago.

6 recordsLinked to original sources

PubMed1990

Multilobated lymphoma of B cell type: a multiparameter investigation.

Multilobated lymphomas were originally described as T-cell neoplasms, but many of B-cell type have subsequently been reported. A case of B-cell origin is reported in which both immunophenotypic and genotypic studies performed on a cell suspension of the lymphoma gave inconclusive and potentially misleading information, while paraffin and frozen section immunohistologic studies, as well as genotypic studies performed on DNA obtained from snap-frozen tissue, were definitive. Thus, this case illustrates some of the problems that may be encountered using cell suspensions as a source for immunophenotypic, and even the much more sensitive genotypic, studies.

Antigens, CD↗

PubMed1989

Gleason grading of prostatic adenocarcinoma on fine-needle aspiration.

We attempted to use the Gleason grading scheme on fine-needle aspiration (FNA) of the prostate by examining 31 prostate aspirates that had concomitant surgical pathology tissue for correlation. A set of criteria is delineated in which it is proposed that Gleason grading is possible on FNA of the prostate by evaluating aspirated tissue fragments. Cell cytology, including qualitative nucleolar appearance, is not significantly helpful in this approach.

Adenocarcinoma↗

PubMed1987

Immunohistochemical classification of acute leukemias using peripheral blood smears.

Immunophenotypic classification of the acute leukemias (AcL) is of well documented value in those of lymphoid or uncertain origin and of increasing importance in those of nonlymphoid origin. Most of these studies have been performed on viable cell suspensions. To study the efficacy of a simpler immunohistochemical approach to the classification of the acute leukemias requiring only peripheral blood smears, 15 AcL (including three CGL-BC) were studied using an immunoalkaline phosphatase method and a panel of anti-lymphoid and anti-myeloid monoclonal antibodies. Routine cytochemistries were also performed (Sudan black, PAS). Using immunohistochemistry, five cases marked as common ALL (four were undifferentiated by cytochemistry, one ALL), eight cases as ANLL (all ANLL by cytochemistry) and two cases marked only with anti-HLA-DR (AUL by cytochemistry). These results show that immunophenotypic analysis of AUL, ALL and ANLL can be successfully performed even when only air dried peripheral blood smears are available.

Histocytochemistry↗

PubMed1987

Acute megakaryocytic leukemia with myeloid/monocytic differentiation.

Acute megakaryocytic leukemia is a rare form of acute nonlymphocytic leukemia that occurs with increased frequency in patients with Down's syndrome. Herein, we report a child with Down's syndrome who presented with a large retroperitoneal mass due to acute megakaryocytic leukemia. Immunohistochemical stains of the tumor cells also demonstrated evidence of myeloid/monocytic differentiation, with positivity for alpha-1-antitrypsin, alpha-1-chymotrypsin, and lysozyme. The significance of this phenotypic heterogeneity is unclear and awaits further studies of similar cases.

Acute Disease↗

PubMed1987

Do pathologic features predict prognosis in diffuse large B-cell lymphoma?

We analyzed 47 immunologically confirmed cases of diffuse large B-cell lymphoma (DLBL) to determine whether the histologic type, surface immunoglobulin (Ig) phenotype, or mitotic rate predicted the clinical outcome. All patients were uniformly staged and uniformly treated with one six-drug protocol. Seventeen cases were subclassified as the noncleaved cell type (DLBL-NC), 23 cases as the immunoblastic type (DLBL-IBL), and 7 cases as other follicular center cell (FCC) types (DLBL-O). The predicted two-year actuarial survival for patients with DLBL-O (82%) was significantly longer than for those with DLBL-NC (35%; p = 0.05). The immunologic phenotype and mitotic rate also predicted the clinical outcome. The predicted two-year survival for patients with DLBL having a surface IgM phenotype (29%; p = 0.06), and the two-year survival for patients with FCC-derived DLBL (DLBL-NC plus DLBL-O) having a surface IgG phenotype (80%) was significantly longer than for those with a surface IgM phenotype (0%; p = 0.02). Similarly, the two-year survival for patients with DLBL having less than 30 mitoses (68%) was significantly longer than for those having greater than or equal to 30 mitoses per 10 high-power fields (28%; p = 0.01). These findings are considered preliminary, and additional studies of a large number of similar patients are necessary for their confirmation.

Actuarial Analysis↗

PubMed1985

Follicular center-cell lymphoma with plasmacytic differentiation, monoclonal paraprotein, and peripheral blood involvement. Recapitulation of normal B-cell development.

A patient with a nodular and diffuse small-cleaved follicular center-cell lymphoma that exhibited definite plasmacytic differentiation, a related monoclonal gammopathy, and circulating population of small lymphocytes is presented. Aside from showing that the presence of numerous plasma cells is not a reliable criterion for the diagnosis of a reactive follicular proliferation, the case is an example of a lymphoma without a block in maturation. The "neoplastic" B-cells show development to follicular center cells and beyond, to functioning plasma cells, and probably also to recirculating "memory" cells. It also suggests that plasmacytoid lymphocytic lymphomas (Lukes-Collins classification) might represent a heterogeneous group of lymphoid neoplasms with some closely related to follicular center-cell lymphomas and others more closely related to small lymphocytic lymphoma/B-cell chronic lymphocytic leukemia.

Antibodies, Monoclonal↗