[Structure of bacteriophages responsible for conversion phenomena in Salmonella].
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Biomedical subjects
Publications and source records attributed to J F Vieu.
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Eighteen phages were studied by electron microscopy. They belonged to eight morphotypes representing the Myoviridae, Siphoviridae and Podoviridae families of tailed phages. Twelve phages were members of known species and six phages belonged to three new species. Results correlated closely with serological and DNA-DNA hybridization data. Induced and non-induced propagating bacteria were investigated for lysogeny. All strains produced R-type pyocins, phage-like particles or inhibitory reactions. The identity of typing phages should be controlled periodically.
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Eighty hospital strains of P. aeruginosa, isolated in Alexandria, were examined with the aid of three epidemiological markers (serotype, lysotype and O. N. P. G.-test). The most frequent serotypes in Alexandria are 0:11, 0:12, 0:2, 0:4, 0:6 et 0:1; this situation is different from those reported in European countries and in U. S. A. Sixty-five lysotypes were found and the strains of serotype 0:11 fall in fifteen lysotypes. One strain 0:12 was found O. N. P. G. positive.
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The pyocin-typing of 448 Ps. aeruginosa from several Hospitals in Lisbonne has been done with the Gillies and Govan method; 69% Portuguese strains are in pyocinotypes n degrees 1, 3, 5 and 7; 16 % are in 24 other pyocinotypes. Four new pyocinotypes were found in a group of 34 atypical strains. The relationship between serotype and pyocinotype was investigated and the most frequent serotypes in Portugal (0:11; 0:6; 0:3) has been subdivided into numerous pyocinotypes with epidemiological significance.
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From 1961 to 1975, researches on salmonellosis have been carried out in Hospital Grall, in Saigon, mainly during two periods (1961-1964, then 1971-1975). 607 strains of Salmonella have been isolated for these 15 years (558 S. typhi and 49 Salmonella, among several other serotypes). As typhoid fever is most important, the authors have studied this disease very carefully. The most efficacious technique of isolating is hemoculture. With coproculture, the results are disappointing. Scarcely, some strains have been found in samples of various origins. Indirect diagnosis methods are less interesting. They may induce only a presumptive diagnosis. S. typhi is always predominant : 86,5 p. 100 from 1961 to 1964 and 96,1 p. 100 from 1972 to 1975. Diffusion of this germ during the second period is linked to the occurring of resistance to chloramphenicol. Some other serotypes are observed scarcely. The lysotypes have been determined for almost all the strains (202, then 323, during the two phases of this study). From 172 to 1975, 24 different lysotypes have been found. Lysotype I + IV is predominant (59 p. 100), but the incidence of D 6 and of 46 (6,8 p. 100) is somewhat high, if it is compared to the others. Lysotype 46 has been observed only since 1971. It has been spreading up to now in South East Asia. Stretching of this lysotype, out of its original focus, is an important epidemiological character. In 1972, resistance of S. typhi to chloramphenicol appeared and it has been increasing mostly during the following years. From 1972 to 1975, 211 strains on 288 were found resistant (highest point in 1975 : 85,4 p. 100). It was a multiresistance to 4 antibiotics (antibiotype : CTSSu). This pehnomenon of plasmidic resistance was investigated in Institut Pasteur (Paris). Many lysotypes have been involved in resistance (14 among 24). Clinical study has been realized for 189 patients. During the typical period of the disease, fever is the major symptom. More scarcely, other clinical features are observed. Various complications have been related. The evolution of the disease has been generally satisfactory, as far as mortality (less than 2 p. 100), sequelae (1 p. 100) and relapses (2 p. 100) are concerned. Clinical course has not been modified by resistance of the germs.
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3720 human strains of P. aeruginosa from Egypt, France, Greece, Iracq and Portugal were studied by serotyping, phage typing, and ONPG-test. The comparison of the results with the data of the scientific literature suggests that three classes of P. aeruginosa O groups can be characterized on the basis of their geographical distribution in the world: 1 degree Nine serogroups with worldwide distribution: 0:1, 0:2, 0:3, 0:4, 0:5, 0:6, 0:8, 0:9 and 0:11. 2 degrees Five serogroups absent (or very rare) in a few countries but present in others: 0:7, 0:10, 0:12, 0:13 and 0:16. 3 degrees Two serogroups very rare in all countries: 0:14 and 0:15.
207 Salmonella typhimurium strains isolated from childrens below three years (190 strains), adult patients (8 strains), health carriers (7 strains) or animals (2 strains) were studied with the biotyping scheme of Cordano, Richard and Vieu (1971). Five biovars were found and 76.8% of the strains were TTR+, Ino+, Tre+, d. Tar- (biovar d). In Irak the epidemiology of the Salmonella typhimurium human infections is associated with an high frequency of strains of Salmonella typhimurium 0:5- (var. Copenhagen), biovar d.
The distribution of phage-types was studied among 434 strains of S. typhi from Bagdad, Amara and Bassora. Biotypes, colicinogeny, tetrathionate-reductase activity (TTR) and susceptibility to Salmonella phage O.I. were also studied. The most frequent phage-types in Irak were A, D1, E1 alpha, F1, M1, O, T, 46 and two other large groups of strains (I + IV and Vi degraded strains). Only 4.38% of S. typhi were TTR-; the strains of phage-type 53 were TTR+. Colicinogeny was demonstrated in 2.5% of S. typhi (lysotype 40, group I + IV and Vi degrades strains). All strains were lysed by Salmonella phage O.I, with the exception of three strains of phage-type T. Comparisons were made with the S. typhi phage-type in Iran and evidence was found for one regional epidemiological community in respect to the distribution of phage-types.
Twenty per cent of Pseudomonas aeruginosa strains isolated in Portugal are non typable with the phage-typing Lindberg system, but most of them may be typed with the aid of a new phage-typing method. This complementary scheme of phage-typing is based on the use of 15 new phages from sewage; 39 phage-types were found among a group of the non-typable strains isolated in several hospitals in Lisbonne. The relations between the serotypes, the pyocinotypes and the new lysotypes are investigated.