Biomedical subjects

J Földi

Publications and source records attributed to J Földi.

52 records · Page 3Linked to original sources

PubMed1981

Erythrocyte alterations in hemoglobin H disease.

This study on erythrocytes in hemoglobin H (Hb H) disease reveals that unstable Hb H is bound to membranes to a greater extent, especially when it forms methemoglobin or is precipitated as inclusion body. The methemoglobin content of these erythrocytes is elevated in spite of a higher activity of NADH-methemoglobin reductase. The ATPase activity is doubled, and the ATP is presumably used for phosphorylation of membrane proteins, which leads to cross-linking of membrane proteins. This assumption could be supported by the observed decrease in non-electrolyte permeability, by increased binding of hemoglobin to the membrane and by polymerisation of membrane proteins detected by SDS-polyacrylamide gel electrophoresis. By means of electron microscopy, it could also be shown that the inclusion bodies are bound to the inner surface of membrane and cause its protrusion. This linkage might be responsible for the observed inhibition of the lateral movement of intramembrane particles.

Cell Membrane↗

PubMed1980

Hemoglobin Pitie-Salpetriere beta 34 (B16) Val replaced by Phe. A new high oxygen affinity variant associated with familial erythrocytosis.

Hemoglobin Pitie-Salpetriere was detected by routine isoelectric focusing. It moved up on isoelectric focusing between hemoglobin A and hemoglobin F, near Hb F. On cellulose acetate strips at pH 8.6, it moved as hemoglobin A. This new variant exhibits a high oxygen affinity associated with familial erythrocytosis. The valine residue in position beta 34 has been replaced by a phenylalanine residue. This residue is involved in the alpha 1 beta 1 contact.

Amino Acid Sequence↗

PubMed1980

The human alpha-globin gene. The protein products of the duplicated genes are identical.

In order to determine whether any heterogeneity exists in the human alpha-globin chain, i.e. whether the products of the duplicated genes are identical, we have determined the total sequence of 14 alpha-globin chains: seven of these were abnormal, while six were normal chains from the same individuals, with one additional sample which consisted of the alpha chains from a normal control. In the individuals heterozygous for an alpha-chain abnormality, the product of a single alpha-gene could be isolated from that of the three others using the differing physicochemical properties of the mutant haemoglobins. In the special situation of a double heterozygosity for an alpha-chain abnormality, the products of the two mutated genes were separated from each other and from the mixture of the products of the two normal genes. They were then investigated independently, this approach increased the precision of our work. During the course of the present investigation, sequence determinations were mainly performed on large fragments of the chains, which were purified exclusively by gel chromatography. In this way mixtures of products of several genes could be studied, thereby overcoming the risk of losing peptides differing slightly in sequence. Such loss may often occur when using ion-exchange procedures to purify small peptides. Our results show the absence of any heterogeneity at the level of the gene products of the duplicated alpha loci. Thus the human alpha-globin chain has to be considered as homogeneous.

Amino Acid Sequence↗

PubMed1980

A new hemoblogin variant in hungary: Hb Savaria - alpha 49 (CE7) Ser replace by Arg.

A hemoglobin variant of slow electrophoretic mobility has been demonstrated in a Hungarian female patient. The abnormal component amounted to about 30% and proved to be a new hemoglobin variant, alpha 49 (CE7) Ser replaced by Arg, which has been named Hb Savaria.

Adult↗

PubMed1975

Duplication of the haemoglobin alpha-gene.

Gene duplication is one of the basic processes underlying evolutionary changes. The gamma-chains of human foetal haemoglobin is coded by multiple structural genes. The delta-chains of Hb A2 can be regarded as a duplication of the beta-locus. We have presented the first evidence for the presence of two major alpha-chain loci in man. The alpha-gene appears to have duplicated recently, since apart of the single point mutations characterizing Hb J-Buda and Hb G-Pest, the two alpha-gene products seem to be identical. Sensitive immunochemical measurement techniques may reveal structural differences which might escape detection by chemical methods based on differences in charge and/or chromatographic behaviour. Anti-alpha-chain sera recognizing the single amino acid substitution in alphaJ-Buda could be raised in rabbits. The anti-alpha-chain sera were found to be more powerful tools for detecting differences in the primary structure of the chain than the immune sera raised against the whole tetramer. None of the immune sera could reliably differentiate Hb G-Pest from Hb A1. The relative strength of complement fixation of the alpha-chains from haemoglobin A1 F and A2 was compared by hybridizing these human haemoglobins with caninehaemoglobin and measuring the quantitative complement fixation of the different hybrids with anti-Hb A1 and anti-alphaA1 rabbit immune sera. No antigenic difference among the alpha-chains from haemoglobins A1, A2 and F could be detected by this method either with anti-A1 or with anti-alphaA1 sera. These results do not exclude the possibility of conformational differences between the alpha-chains in native Hb A and Hb F. The antigenic activity of the alpha-chains of Hb A from normal subjects (alphaA1) and of the alpha-chains of Hb A from a double heterozygote for alphaJ-Buda and alphaG-Pest (alphaA1) were compared by the complement fixation technique. Definite differences could be detected in the relative strength of complement fixation by alphaA1 and alphaA1 with anti-alphaA1 serum. Final decision as to whether alpha-chain duplication is a universal phenomenon or whether it is restricted to only a part of mankind cannot be drawn until the presence of a silent alpha-thalassaemia gene is not excluded in some debated cases by reliable chemical methods. Measurement of alpha-globin genes in Hb H disease with cDNA enriched in alpha-globin sequences provided direct evidence that a non-thalassaemic subject has to have at least four alpha-globin genes per diploid cell.

Chromosome Mapping↗

PubMed1974