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Biomedical subjects

J Filkuka

Publications and source records attributed to J Filkuka.

32 records · Page 2Linked to original sources
PubMed1975

[The surgical problem of experimental liver transplantation in swine].

Lethal causes of 33 experimental animals which survived more than 3 days after liver transplantation were rejection of the graft, thrombosis of vascular stems, bile duct obstruction, and gastric ulcers. Trombogenesis in the vascular stems can be inhibited by application of anticoagulants during and after operation. Obstruction of the bile duct anastomosis can be prevented by the author's previously reported method of drainage. The papilla of the duodenum is spared in order to reduce ascending infection. In no case immunosuppressive treatment was applied. Nevertheless, there was long-term survival of the experimental animals (longer than 1 year) in good general state and with normal sexual functions. Till now the authors could prove no alterations in the descendants that might be caused by the hepatic transplantation in the parents.

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PubMedDate not supplied

The border zone of the early myocardial infarction in dogs; its characteristics and viability.

In open-chest dogs, the left anterior descending coronary artery was ligated for 150 min. The heart was semiserially cut on a cryomicrotome and areas of ischemic damage were visualized by means of glycogen depletion (PAS reaction) and tissue acidosis (a "sandwich" technique with pH indicator dispersed in a layer of gel). The extent of myocardial damage was determined morphometrically. The mass of the glycogen-depleted heart muscle was greater than the mass of the ischemic tissue detected by means of decreased pH (p less than 0.01). The border zone was characterized by glycogen depletion without acidosis. Circulation studies using intravital fluorescein staining have shown that perfusion is partially retained in the border zone; it is assumed that the hypoperfusion triggers glycogenolysis. Nevertheless, the level of perfusion suffices to wash out the acidic end products. Comparison of contrapulsation-treated dogs and untreated dogs shows that the amount of damaged tissue comprising the border zone can be reduced by this therapeutic intervention (p less than 0.02) - in contrast to the acidotic tissue, the amount of which is not significantly influenced. Therefore the border zone contains damaged but still viable muscle cells.

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PubMedDate not supplied

Reperfusion of coronary arteries after temporary occlusion in dogs.

The influence of previous pharmacotherapy or intra-aortic balloon counterpulsation (with thiamine infusion) on the effectiveness of reperfusion of the left anterior descending (LAD) coronary artery after 150 minutes of occlusion has been studied in dogs. The infarct size was determined by morphometric method 20 hours after the onset of ischaemia. Reperfusion 150 minutes after the occlusion of the LAD coronary artery resulted in a smaller infarct; however, no protective effect from pharmacological or mechanical heart support during the period of ischaemia was observed.

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PubMedDate not supplied

Suppression of the progress of necrosis after coronary artery ligation in the dog by pharmacologic interventions.

A study was carried out of a therapy supporting the anaerobic glycolysis and coronary perfusion of ischemic myocardium and preserving its potassium and magnesium contents, which consisted in combined administration of hyaluronidase, glucose, insulin, Tris, methoxamine, and potassium and magnesium aspartate. The drugs were administered to Beagle dogs between 30 minutes and 20 hours after the ligation of the left anterior descending coronary artery. The size of infarction was determined by the Nachlas method, which is based on the detection of activities of dehydrogenases in heart slices. Treated animals showed a reduced infarction size (p less than 0.001). The evidence of the favourable effect of the therapy used was brought also by hemodynamic measurements. The heart rate was slower (p less than 0.02) and the mean arterial blood pressure was higher (p less than 0.001) in treated animals than in control ones (as evaluated at the end of the experimental period). Significant correlation between the infarction size and the heart rate on the one hand and the mean arterial pressure on the other hand was found (r = 0.59, p less than 0.01; r=--0.47, p less than 0.05, respectively).

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