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J Flik

Publications and source records attributed to J Flik.

25 records · Page 2Linked to original sources
PubMed1987

[Transient HIV-antibody positive tests in heart transplantation patients after repeated administration of a CMV immune globulin preparation].

After repeated administration of relatively high doses of a cytomegalovirus immunoglobulin preparation to two patients after heart transplantation, both the ELISA and the immuno-blot tests became temporarily positive (for 21 and 41 days, respectively) to HIV antibodies. The CMV immunoglobulin preparation was demonstrated to have high HIV antibody titres. The latter ran parallel to antibody titres against measles virus, also transferred passively with the immunoglobulin preparation. This makes it unlikely that HIV antibodies had been formed by active immunization from HIV in the immunoglobulin preparation. Within 126 and 176 days, respectively, after the last positive test against HIV antibodies there was no further demonstration of HIV antibodies or of a corresponding illness. There was thus no evidence of an HIV infection having been transmitted by the immunoglobulin preparation.

Antibodies, Viral↗
PubMed1987

Recent influenza virus A infections in forensic cases of sudden unexplained death.

84 forensic necropsy cases with a history of sudden unexpected death and where no acceptable cause of death was found at autopsy (= cases of sudden unexplained death, SUD) were found to have a significantly higher rate of influenza A (H 3 N 2) infection than did matched controls of the general population and a group of forensic necropsy cases with known cause of death (NON-SUD cases). By contrast, the group of SUD cases was found to have no significantly increased infection rate with influenza H 1 N 1 and B virus, parainfluenza viruses, RS virus, adenovirus, and cytomegalovirus. The influenza A associated SUD cases had a significantly higher rate of pathological and histological findings previously described for cases of primary viral pneumonia than did SUD cases without recent influenza A infection and NON-SUD cases. These findings suggest that virological examination of SUD cases could be helpful in order to determine the probable cause of death. A considerable portion of the influenza associated SUD cases occurred during interepidemic influenza periods. Therefore, such cases could be a useful source for monitoring the interepidemic spread of influenza virus.

Autopsy↗
PubMed1987

Serological evidence of recent influenza virus A (H 3 N 2) infections in forensic cases of the sudden infant death syndrome (SIDS).

40 forensic SIDS cases were found to have a significantly higher rate of serologic evidence of recent influenza A (H 3 N 2) infection than did matched controls. In contrast, the SIDS cases had serologically no significantly increased infection rate with influenza H 1 N 1 and B virus, parainfluenza virus, RS virus, adenovirus, and cytomegalovirus. SIDS cases with recent influenza infection had a significantly higher rate of histological findings as described for primary viral pneumonia than did SIDS cases without influenza infection. SIDS cases with recent influenza infection occurred much more frequently during epidemic than during interepidemic influenza A (H 3 N 2) periods. Our results confirm previous reports that SIDS cases have an increased rate of respiratory virus infections. However, they cannot prove a causal relationship between influenza infection and death. Since our SIDS cases comprised 75 per cent of cases aged more than three months, our results pertain essentially to cases of this age group.

Antibodies, Viral↗
PubMed1987

Comparative investigation of monoclonal and polyclonal antibodies directed against strain-specific and common antigenic sites on influenza H1N1 virus hemagglutinin.

Antibodies directed against strain-specific and common antigenic sites of H1N1 influenza virus hemagglutinin were tested comparatively, using monoclonal antibodies raised against strain A/Brazil/11/78 and polyclonal antibodies directed against strains A/Brazil/11/78, A/USSR/97/77, A/PR/301/54, and A/FM/1/47. The patterns of competition between antibodies for adsorption onto homologous virus indicated that the monoclonals comprised antibodies directed to each of the two strain-specific (Sa and Sb) and common antigenic sites (Ca and Cb) of virus hemagglutinin. Polyclonal strain-specific antibodies (SSA) yielded the competition patterns of mixtures of anti-Sa and anti-Sb antibodies and polyclonal common antigen antibodies (CAA) yielded those of mixtures of antibodies directed against sites Ca and Cb, indicating that the polyclonal preparations comprised a similar repertoire of antibodies, as represented by the panel of monoclonals. This conclusion was confirmed by determining, by means of equilibrium filtration, the number of epitopes per homologous virion(s) recognized by antibody preparations and their mixtures. Polyclonal SSA and CAA gave s values not significantly different from those of mixtures of the corresponding monoclonal antibodies. The strains tested were found to possess equivalent numbers of strain-specific and common epitopes per virion. The competition between antibodies was further examined in terms of the additiveness of s values they recognize in simultaneous reactions. No competition was observed for the monoclonal antibody pairs anti-Sa/anti-Ca, anti-Sa/anti-Cb and anti-Sb/anti-Cb, indicating that these antibodies combined with nonoverlapping epitopes. Polyclonal SSA and CAA yielded partial competition. The equilibrium constants (K) of comparable SSA and CAA were within the same range, and SSA and CAA did not influence their binding avidity when allowed to react simultaneously with homologous virus.

Animals↗
PubMedDate not supplied

Management of herpes simplex virus type 1 pneumonia following liver transplantation.

Interstitial pneumonia caused by Herpes simplex virus type 1 (HSV-1) is a severe complication of orthotopic liver transplantation (LTX). The records of patients were reviewed who had an LTX at the age of 16 years or older between 1991 and 1994 with a mean follow-up of 21 months (range, 10 to 44 months). Six patients were included who had fever of > 38 degrees C, deterioration of arterial blood gases, radiological evidence of interstitial pneumonia and proof of HSV-1 in bronchoalveolar lavage fluid. All patients were anti-HSV-IgG positive before LTX. All patients were successfully treated with intravenous acyclovir, mechanical ventilation and reduced immunosuppression. Three patients who received cyclosporin A had a rejection which was successfully treated by switching to FK 506. Four patients were discharged in good health. One patient died 36 months after LTX of an unrelated cause. One patient died of urosepsis on postoperative day 139. Acyclovir together with mechanical ventilation and reduced immunosuppression proved to be an effective treatment for HSV-1 pneumonia following LTX.

Acyclovir↗