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Biomedical subjects

J Foley

Publications and source records attributed to J Foley.

At least 55 records · Page 3Linked to original sources
PubMed1984

Peanut lectin agglutinin and alpha-lactalbumin. Binding and immunohistochemical localization in breast tissues.

We analyzed 31 breast lesions immunohistochemically for alpha-lactalbumin (ALA) production and T-antigen presence as defined by peanut lectin agglutinin (PNA). Most cases of well-differentiated infiltrating carcinomas (14 of 17) and of poorly differentiated carcinomas (two of four) showed positive immunoreactivity for ALA; ALA was also localized in three cases of normal breast tissues, in two fibroadenomas, and in five intraductal carcinomas. The PNA binding pattern was primarily along the luminal cytoplasmic cell membrane in normal breasts and in fibroadenomas. We saw both cell membrane and cytoplasmic binding in carcinomas. In seven cases of primary and metastatic carcinomas to axillary lymph nodes, both primary and metastatic tumors expressed T antigen and produced ALA. These studies indicate that ALA can be used as a diagnostic marker in breast carcinoma and that the PNA binding pattern in normal and benign breast tissues is primarily at the luminal cytoplasmic cell membrane, whereas both cytoplasmic and cell membrane binding are present in carcinomas. The production of ALA does not appear directly related to T-antigen expression or to metastatic disease.

Adenofibroma↗
PubMed1978

Vestibulogenic imbalance.

Vestibular lesions may cause imbalance that long outlasts vertigo. Photographic analysis in five cases showed defects not only of the tilting reactions, which are of labyrinthine origin, but also of certain other postural reactions, notably the counterpoising and protective stepping reactions. All of these reflexes are unconscious and consequently such patients are rendered unstable in several ways simultaneously and may have difficulty describing their symptoms.

Adult↗
PubMed1978

Evidence for increased protease activity secreted from cultured fibroblasts from patients with pseudoxanthoma elasticum.

Abnormal polyanion metabolism in cultured fibroblasts from patients with pseudoxanthoma elasticum (PXE) was studied by incubating normal and PXE fibroblasts in culture medium containing 35SO4 for 96 hr and measuring differences in secreted 35SO4-labelled proteoglycans (35S-PG). PXE medium contained less high molecular weight (MW) 35S-PG and more low MW 35S-labelled molecules than normal medium. Addition of diisopropylfluorophosphate (DFP), a serine protease inhibitor, to the media after the 96 hr incubation resulted in no change in the MW distribution of 35SO4-labelled molecules in normal media. However, DFP treated PXE medium contained significantly more high MW 35S-PG than either untreated PXE medium or DFP treated normal medium. High MW 35S-PG was isolated from PXE fibroblast culture medium and incubated with serum free medium from either normal or PXE fibroblast cultures. There was significantly more degradation of this 35S-PG to low MW 35S-labelled molecules by PXE medium than by normal medium. 32P-DFP, which binds to the active site of serine proteases, was added to serum free medium from normal and PXE cultures. The specific radioactivity of PXE medium was 4 times greater than that of normal medium. These lines of evidence are consistent with the hypothesis that a biochemical defect in cultured PXE fibroblasts is the increased secretion of a serine protease that can degrade sulfated proteoglycans.

Fibroblasts↗
PubMed1978

Antibody response of horses to Mycoplasma mycoides subsp capri.

In horses given whole cultures or cells of Mycoplasma mycoides subsp capri (by subcutaneous and intravenous injections), antibody responses were measured by serologic procedures. During an immunization period of 22 weeks, horses produced an antiserum that was used to identify M mycoides subsp capri by agglutination, complement-fixation, and fluorescent antibody (FA) tests, but not by the growth-inhibition test. Horses that were injected with whole cultures of M mycoides subsp capri responded better than horses that were injected with only cells, ie, antibodies were detectable sooner by agar gel diffusion and FA tests and the serums displayed more bands of precipitation. The FA reagent was stable during lyophilization and storage at 5 C for 60 days.

Agglutination Tests↗
PubMed1975

Metabolism of dog gastric mucosa. Nucleotide levels in parietal cells.

Adenine and pyridine nucleotide levels as well as those of phosphate, phosphocreatine, lactate, pyruvate, beta-hydroxybutyrate, acetoacetate, glucose, and glycogen were measured in histologically defined parietal and mucous cell sections of biopsies of dog gastric mucosa at rest, and in various secretory states. As a result of stimulation of secretion, there appeared to be no change in adenine nucleotide levels, or phosphocreatine, but there was a rise in inorganic phosphate and a fall in phosphorylation potential. However, there was a marked increase in NADH, but no change in NADPH with onset of acid secretion. The increase in the lactate to pyruvate ratio showed that the increased NADH level occurred in the cytoplasm and these data are discussed with reference to change in cell pH.

Acetates↗