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Biomedical subjects

J Forsberg

Publications and source records attributed to J Forsberg.

13 recordsLinked to original sources
PubMed2001

Molecular recognition in thylakoid structure and function.

In photosynthesis, light-harvesting chlorophyll molecules are shunted between photosystems by phosphorylation of the protein to which they are bound. An anchor for the phosphorylated chlorophyll-protein complex has now been identified in the reaction centre of chloroplast photosystem I. This finding supports the idea that molecular recognition, not membrane surface charge, governs the architecture of the chloroplast thylakoid membrane. We describe a model for the chloroplast thylakoid membrane that is consistent with recent structural data that specify the relative dimensions of intrinsic protein complexes and their dispositions within the membrane. Control of molecular recognition accommodates membrane stacking, lateral heterogeneity and regulation of light-harvesting function by means of protein phosphorylation during state transitions--adaptations that compensate for selective excitation of photosystem I or photosystem II. High-resolution structural description of membrane protein-protein interactions is now required to understand thylakoid structure and regulation of photosynthesis.

Adenosine Triphosphatases↗
PubMed2001

Redox signalling in chloroplasts and mitochondria: genomic and biochemical evidence for two-component regulatory systems in bioenergetic organelles.

Redox chemistry is central to the primary functions of chloroplasts and mitochondria, that is, to energy conversion in photosynthesis and respiration. However, these bioenergetic organelles always contain very small, specialized genetic systems, relics of their bacterial origin. At huge cost, organellar genomes contain, typically, a mere 0.1% of the genetic information in a eukaryotic cell. There is evidence that chloroplast and mitochondrial genomes encode proteins whose function and biogenesis are particularly tightly governed by electron transfer. We have identified nuclear genes for 'bacterial' histidine sensor kinases and aspartate response regulators that seem to be targeted to chloroplast and mitochondrial membranes. Sequence similarities to cyanobacterial redox signalling components indicate homology and suggest conserved sensory and signalling functions. Two-component redox signalling pathways might be ancient, conserved mechanisms that permit endogenous control over the biogenesis, in situ, of bioenergetic complexes of chloroplasts and mitochondria.

Amino Acid Sequence↗
PubMed2001

Heat stress response in pea involves interaction of mitochondrial nucleoside diphosphate kinase with a novel 86-kilodalton protein.

In this work we have further characterized the first mitochondrial nucleoside diphosphate kinase (mtNDPK) isolated from plants. The mitochondrial isoform was found to be especially abundant in reproductive and young tissues. Expression of the pea (Pisum sativum L. cv Oregon sugarpod) mtNDPK was not affected by different stress conditions. However, the pea mtNDPK was found to interact with a novel 86-kD protein, which is de novo synthesized in pea leaves upon exposure to heat. Thus, we have evidence for the involvement of mtNDPK in mitochondrial heat response in pea in vivo. Studies on oligomerization revealed that mtNDPK was found in complexes of various sizes, corresponding to the sizes of e.g. hexamers, tetramers, and dimers, indicating flexibility in oligomerization. This flexibility, also found for other NDPK isoforms, has been correlated with the ability of this enzyme to interact with other proteins. We believe that the mtNDPK is involved in heat stress response in pea, possibly as a modulator of the 86-kD protein.

Biopolymers↗
PubMed1998

High prevalence of Chlamydia pneumoniae DNA in peripheral blood mononuclear cells in patients with cardiovascular disease and in middle-aged blood donors.

Nested polymerase chain reaction (nPCR) demonstrated the presence of Chlamydia pneumoniae-specific DNA in peripheral blood mononuclear cells (PBMC). PBMC samples were obtained from 103 consecutive patients (62 male, 41 female) aged 22-85 years (mean, 64) admitted for coronary angiography because of suspected coronary heart disease and from 52 blood donors (43 male, 9 female) aged 40-64 years (mean, 49). Of the 101 evaluable patients, 60 (59%) were identified by nPCR assay as C. pneumoniae DNA carriers; C. pneumoniae-specific microimmunofluorescence (MIF) serology confirmed exposure to the bacterium in 57 (95%) of the 60 nPCR-positive patients. Among the 52 blood donors, the nPCR assay identified 24 (46%) C. pneumoniae DNA carriers, all of whom were positive by C. pneumoniae-specific serology. Thirty-two patients (32%) and 23 blood donors (44%) were MIF antibody-positive but repeatedly nPCR-negative; Bartonella henselae- or Bartonella quintana-specific antibodies were not detected among any of these subjects. In this study, C. pneumoniae DNA was common in PBMC of patients with coronary heart disease and in middle-aged blood donors.

Adult↗
PubMed1997

Periapical radiolucencies as evaluated by bisecting-angle and paralleling radiographic techniques.

Two groups of patients were included in the study. The first group consisted of patients who received root canal treatment of single-rooted teeth (n = 63). The completed roof fillings were exposed to two different radiographic techniques, the paralleling and the bisecting-angle technique. The second group consisted of 1-year review radiographs of patients who had received apicectomies of single-rooted teeth (n = 105). Three observers examined the radiographic images. First, they were asked to identify teeth with a normal apical condition and those with an apical radiolucency. Thereafter pairs of radiographs were compared; cases judged as normal by all observers were excluded. The observers were now asked to ascertain whether the apical radiolucency was largest in the first image, the apical radiolucency was largest in the second picture or both radiolucencies were the same size. Both intraobserver and interobserver agreement, calculated as Cohen's kappa, was high with respect to the presence of lesions within both samples and it was at the same level for both radiographic techniques. The evaluation of the size of the lesions proved to be more inconsistent. Kappa values were in the range 0.38-0.71 for intraobserver comparisons and in the range 0.25-0.48 for interobserver comparisons. No significant difference was found between the size of lesions as recorded by the two techniques (P > 0.05). It is concluded that, when correctly adjusted the bisecting-angle technique and the paralleling technique provide similar diagnostic results.

Alveolar Bone Loss↗
PubMed1994

Radiographic simulation of a periapical lesion comparing the paralleling and the bisecting-angle techniques.

Sixty apical areas of single-rooted teeth were examined in this study. A simulated periapical lesion was produced using an acrylic sphere (diameter 2.0 mm), the surface of which was covered with a thin layer of zinc cement. The sphere was placed in close contact with the apical foramen using a thin orthodontic wire inserted into the apical portion of the root canal. The correctly adjusted parallel projection depicted the simulated lesion with a very low degree of error. Overangulation of the central beam with maintenance of parallelism between tooth and film resulted in a moderate degree of magnification. When correctly adjusted, the bisecting-angle technique produced images with almost the same accuracy as the paralleling technique. Over-angulation resulted in most images showing a reduced size of the simulated lesion. Moderate under-angulation most often recorded the correct dimension of the globe. In some cases a magnified image of the sphere was produced. Generally, this was associated with a palatally curved root. The investigation thus clearly indicated that the paralleling technique provides the most reliable information about the extent of a pathological process.

Humans↗
PubMed1992

A method for experimental dental radiography.

An apparatus was designed for experimental dental radiography, which permitted separate adjustment of the subject, target and film. The precision of the apparatus was tested by radiographing a test subject, consisting of small steel globes embedded in a plastic plate. The subject was placed in the apparatus and exposed 30 times on dental X-ray film with different adjustments of angles and distances. All exposures were repeated after readjustment of the same form. Three linear distances between pairs of the projected steel globes were selected and measured on each radiograph. Depending on the different orientations of film, target and subject, the distances measured ranged from 9.0-11.1 mm, from 7.0-10.7 mm and from 12.7-17.3 mm, respectively. From the measurements on the radiographs the error of method was calculated to be 0.07-0.08 mm and the error of measurement to be 0.05-0.06 mm. The precision of the apparatus was thus found to be adequate for measurement of differences in small, but clinically relevant, distances.

Dental Pulp Cavity↗
PubMed1987

Radiographic reproduction of endodontic "working length" comparing the paralleling and the bisecting-angle techniques.

The reproduction of a metal indicator simulating a root canal instrument at the apex of a tooth was examined as a function of paralleling, modified paralleling, and bisecting-angle radiographic techniques. Radiographs exposed according to the paralleling and modified paralleling principles demonstrated good correlation between the radiographic position of the metal indicator and its position on direct inspection. The bisecting-angle technique, either correctly or incorrectly angulated, produced the least accurate results. The apical location of the metal indicator was significantly more reliable with the paralleling principle than with the bisecting-angle technique (p less than 0.05).

Equipment Design↗
PubMed1978

Interaction of ascorbate with the radioprotective effect of mercaptoethylamin. An exploratory study in mice, whole animals and cell cultures.

The injection of ascrobate together with cysteamine (beta-mercaptoethylamin or MEA) was shown to cause a partial reversion of the radioprotective action of MEA in mice, and simultaneously of the suppressive action of MEA on RNA synthesis in bone marrow cells. In mouse spleen lymphocytes stimulated by concanavalin A in vitro, MEA and ascorbate exhibited a strong antagonism, neutralizing each other's inhibitory action on RNA synthesis. The latter effect failed to appear after chelation of trace metals, and it is indicated that the ability of ascorbate to counteract the effects of MEA on radiosensitivity and metabolism requires the formation of oxidized products, probably monodehydroascorbate, in agreement with previous observations on bacteria.

Animals↗