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Biomedical subjects

J Frei

Publications and source records attributed to J Frei.

At least 37 records · Page 2Linked to original sources
PubMed1988

Dialyzed polymorphonuclear neutrophil oxidative metabolism during dialysis: a comparative study with 5 new and reused membranes.

Dialyzed neutrophils were isolated at time 0, 5, 15 and 60 min after the onset of hemodialysis in patients successively treated on 5 new and reused membranes, that is cuprophan (CU), cellulose acetate (CA), polysulfone (PS), polycarbonate (PC) and polyacrilonitrile (PAN). Production of oxygen radicals was monitored by luminol and lucigenin-enhanced chemiluminescence (CL). During dialysis with CU and PC, cells remaining in circulation at the maximum neutropenia showed a significant decrease of luminol-enhanced CL, whether stimulated with opsonized zymosan or phorbol myristate acetate. This defect was transient and the responses normalized at 60 min or upon reuse of the membranes. Among the other membranes tested, only cells collected during the first use of PS showed an impaired CL response to phorbol myristate acetate, but not to opsonized zymosan. CL again normalized upon reuse. At 5 min of dialysis with each membrane, a plasma factor appeared that was able to stimulate oxygen radical production by autologous dialyzed and control cells. A dissociation between the oxidative responses of dialyzed neutrophils and neutropenia was observed depending on the nature of the membranes, suggesting that neutropenia is a multifactorial process in which oxygen radical production appears as an early disturbance.

Aged↗
PubMed1987

[Music and hearing].

Explore the source record for details and available documents.

Acoustic Stimulation↗
PubMed1986

[Severe, acute bleeding ulcer: which criteria are decisive for therapeutic procedures?].

Prognostic and therapeutic implications of acute severe gastrointestinal bleeding were retrospectively analyzed, on the basis of history, clinical and endoscopic findings, in a group of 50 consecutive patients with endoscopically documented ulcer of the stomach or duodenum. In patients with hemorrhagic shock and a history of melena and/or hematemesis represents the most important indication for urgent action. In this setting the surgical approach remains the therapy of choice if other measures (endoscopic coagulation, somatostatin application) are unable to prevent further bleeding. Except in situations with acute arterial bleeding, the endoscopic finding itself is not decisive in selecting the therapeutic procedure.

Combined Modality Therapy↗
PubMed1986

The polycarbonate hemodialysis membrane: neutrophil, platelet, complement and chemiluminescence kinetics during first and second use.

In a clinical comparative cross-over study of 12 chronically hemodialyzed patients, the polycarbonate hemodialyzer membrane disclosed the following features: A drop in neutrophil count to 45 +/- 11% predialysis values occurred 15 min after the start of dialysis. Plasmatic C3d increased at the initiation of dialysis simultaneously with the nadir of neutropenia but also towards the end of dialysis when neutrophil count was normal. An inhibition of chemiluminescence of neutrophils isolated 5 and 15 min after the start of dialysis was noted. Reuse of the membrane induced during the second use a significant aggravation of neutropenia (36 +/- 23% at 15 min), a drop in platelet count (91 +/- 10% at 15 min) and a return to normal of chemiluminescence. When compared to the results observed with cuprophane and polyacrilonitrile membranes in the sam patients, the polycarbonate membrane seems to possess intermediate biocompatibility properties.

Acrylic Resins↗
PubMed1985

[ Multiple facets of the polynuclear neutrophil and study of its functions].

Blood polymorphonuclear leukocytes are professional phagocytes that eliminate from the body any invading microorganisms, but also release undesirable inflammatory mediators into the surroundings. The normal neutrophil function is dependent on the integration of chemotaxis, phagocytosis, degranulation and oxidative metabolism. Available in vitro assays have allowed the quantitative evaluation of each separate function and the description of congenital, acquired or transitory neutrophil abnormalities.

Chemotaxis, Leukocyte↗
PubMed1984

[Arterial aneurysm of the liver with arterio-portal fistula: treatment using embolization].

Aneurysms of the hepatic artery are rare and may present multiform clinical pictures. Prompt diagnosis is important because life threatening complications can be treated by relatively simple measures. A case of posttraumatic intrahepatic aneurysm of the hepatic artery with arterioportal fistula and successful transcatheter embolisation is reported. The present state of the art is outlined and indications for diagnosis and therapy are presented.

Aneurysm↗
PubMed1984

Oxygen metabolism of phagocytosing human polymorphonuclear leucocytes in diabetes mellitus.

Zymosan stimulated oxygen metabolism was investigated in polymorphonuclear leucocytes (PMN) from 6 diabetic patients. Oxygen uptake and superoxide production were continuously measured in the presence of autologous or control serum and non-opsonized zymosan, or in the absence of serum and preopsonized zymosan. The only significant impairment in the diabetic cells studied was a lower oxygen uptake in the presence of autologous serum. This defect was normalized by addition of control serum or by omitting the serum and stimulating with opsonized zymosan. In the absence of serum, the oxygen consumption was markedly diminished in only one subject, whereas two subjects showed a decrease of superoxide production in the presence of control serum. An inverse correlation between fasting glucose concentration and oxygen uptake could be demonstrated. However, exposure of normal PMN to hyperglycemic glucose concentration in vitro did not significantly alter their oxygen metabolism, suggesting that glucose alone could not be the only factor responsible for the impaired oxygen consumption in diabetic cells.

Aged↗
PubMed1984

[Hysteria: problems of definition and evolution of the symptomatology].

What is the evolution of hysteria, now that the spectacular crises of previous times tend to occur less frequently? Are hysterical manifestations disappearing or do they take other forms? This question raises semantic and phenomenological problems concerning hysteria. A review of the literature shows the complexity and precariousness of some definitions, the question raised by associated organic disorders and the compounding influences of other nosological entities, such as depression and psychosomatic disorders. Clinical histories from the records of a psychiatric hospital for the period 1910-1929 were compared with those for the period 1970-1980. The results of the study were surprising with respect to the similarity of epidemiological profiles. If it can be concluded that hysterical manifestations are not disappearing, then the study confirms the evolution of symptomatology which, especially in women, takes atypical forms of depressive, neurovegetative or psychosomatic disorders. In males, hysterical symptoms are of a more acute nature and frequently occur in dependent, immature or infantile personalities, whilst in the past they were more chronic and frequently associated with more serious personality disorders.

Adult↗
PubMed1983

Cuvette and flow system for simultaneous determination of optical density and oxygen concentration.

The major components of the device for semiautomatic measurement of oxygen consumption and optical density changes are a thermostated glass reservoir of 50-250 ml for the reaction mixture, a roller pump to transport the reaction mixture from the reservoir into the measuring cuvette, and a stoppered glass cuvette equipped with a Clark type electrode. The glass cuvette (0.525 ml) is square shaped (the interior section 6 X 6 mm). The lower part housing the magnetic stirrer has a side aperture for the oxygen electrode. The upper part of the cuvette is cylinder shaped. The stopper is fitted in this part. The stopper made of lucite protrudes 15 mm into the cuvette. The access and the removal of the reaction mixture to/from the cuvette is ensured by three different channels machinated into the body of the stopper. The device adaptable to Eppendorf spectralline photometer may be used in the study of oxidative phosphorylation on intact mitochondria or submitochondrial particles, or of any oxygen consuming system in which one reagent or reaction product can be monitored photometrically.

Densitometry↗
PubMed1980

Energetic metabolism of leukocytes. XI. Presence and function of creatine kinase in leukocytes.

Creatine kinase activity could be demonstrated in the cytosol of macrophages and polymorphonuclear leukocytes from guinea pig peritoneal exudates, in circulating mononuclear cells of blood donors and of a preleukemic patient, but not in human circulating polymorphonuclear cells. According to immunoinhibition assays most of the creatine kinase activity of human monocytes (preleukemic patient) and lymphocytes can be assigned to the MM and BB isoenzymes, respectively. Both isoenzymes have been observed in human lymphocytes by electrophoresis; with the help of the same procedure the predominance of creatine kinase BB has been demonstrated in the cells of guinea pig peritoneal exudates. The possible function of creatine kinase in leukocytes is discussed.

Animals↗
PubMed1979

The energy metabolism of the leucocyte. X. Kinetics of oxygen consumption during phagocytosis by polymorphonuclear leucocytes. A photometric method.

A simple micromethod for quantitative assay of the oxygen consumption by phagocytosing human neutrophils had been described. The continuous assay shows a lag time between the addition of zymosan and the maximal rate of oxygen uptake, which is a measure of the serum opsonic activity. Several factors can alter the oxygen uptake and also the delay of response.

Amobarbital↗