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Biomedical subjects

J G Anderton

Publications and source records attributed to J G Anderton.

3 recordsLinked to original sources
PubMed1996

Increased susceptibility to membrane lipid peroxidation in renal failure.

The susceptibility of membrane lipids to peroxidation in renal failure was investigated. A fluorescent polyunsaturated fatty acid probe, cis-parinaric acid, showed that mononuclear leucocytes from both chronic renal failure and haemodialysis patients had increased susceptibility to membrane lipid peroxidation compared with normal controls. The effect of oxidant stress on the physical properties of the cell membrane was also investigated by measuring fluidity with the probes diphenylhexatriene and trimethylaminodiphenylhexatriene. Oxidant stress caused a significant increase in membrane fluidity of mononuclear leucocytes from normal controls, which was greater than that seen in renal failure. The effect of oxidant stress on the physical properties of the cell membrane was not simply determined by the susceptibility of the membrane lipids to peroxidation.

Cell Membrane↗
PubMed1989

MHC class II antigen expression is not induced on murine epidermal keratinocytes by interferon-gamma alone or in combination with tumour necrosis factor-alpha.

Epidermal keratinocytes are induced to express MHC class II molecules in a variety of disease states associated with immune activity. To investigate the mechanism of this process we have exposed murine and rat keratinocytes to a variety of lymphokines and monitored changes in their MHC molecule expression. Murine cultured keratinocytes were treated with recombinant interferon-gamma (IFN-gamma), and MHC antigen expression quantified by flow cytometry. IFN pretreatment resulted in the up-regulation of class I molecule expression, but no class II expression was detected. In addition, cultured murine keratinocytes exposed to a combination of recombinant tumour necrosis factor (TNF)-alpha and IFN-gamma, or crude lymphocyte supernatants, failed to show positive membrane staining for class II molecules. However, rat keratinocytes cultured under conditions identical to murine cells were induced to express class II molecules after IFN-gamma pretreatment. The inability of IFN to induce class II expression on murine keratinocytes appears not to result from cell culture, as subcutaneous injection of IFN fails to induce epidermal class II antigen expression. However, class II expression can be induced on rat epidermis in vivo. Thus, the response of epidermal keratinocytes to IFN-gamma appears to show species variation.

Animals↗