J G Christiaanse | PubMed Health

PubMed1982

An epiiluminator/detector unit permitting arc lamp illumination for fluorescence activated cell sorters.

The application of arc lamps to flow cytometers is discussed and epiillumination for jet-in-air cell sorters is introduced. An epiilluminator/detector unit equipped with a mercury arc lamp constructed for a commercially available cell sorter is described. Experiments in which laser and mercury arc lamp illumination were compared show that the signal-to-noise ratio for the arc lamp illumination is predominantly limited by shot noise from constant light backgrounds due to reflected excitation light and ambient light. Arc lamp illumination can be used for the sorting of highly fluorescent objects such as cells stained for DNA by for example: ethidium bromide, propidium iodide, or the Hoechst dyes. The simultaneous employment of mercury arc and laser light sources as an inexpensive dual wavelength system is discussed.

Cell Separation↗

PubMed1981

The look-up table: a classifier for cell sorters.

Currently used classifiers for flow cell sorters are described and the look-up table is introduced as such. An experimental setup of a look-up table classifier added to a commercially available cell sorter, under control of a microcomputer is described. The contents of the look-up table are obtained from contours of regions of interest. These regions are defined by the user who designates the areas on a video color display of the bivariate flow data. Since most cell clusters form elliptic or ovoid shapes in a multi-dimensional feature space this method enables a better matching of window shapes to cluster outlines, compared to conventional sort decision circuitry.

Cell Separation↗