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Biomedical subjects

J G Cox

Publications and source records attributed to J G Cox.

18 recordsLinked to original sources
PubMed1996

Comparison of three immunoassay kits for serum thyroglobulin in patients with thyroid cancer.

OBJECTIVES: This study describes the evaluation of performance of three immunoassay kits [Sanofi-Pasteur, IRMA, with five monoclonal antibodies; Wallac-Delfia, FIA, with double monoclonal antibodies; and Diagnostic Products Corporation (DPC), RIA, with polyclonal antibodies] for serum thyroglobulin in patients with thyroid cancer. Specific aims were (a) to recognize a method with minimal interference, by comparing analyte recoveries, from thyroglobulin autoantibodies (TgAb) that can be present in some thyroid cancer patient samples, (b) to correlate individual results with those from Mayo Medical Laboratories (MML) (current referred out assay service used by our institute), and (c) to compare assay precision. METHODS: In evaluating performance of the three immunoassays, procedures were followed as recommended by the respective manufacturers. Parallel comparison of the three immunoassays with the MML procedure was performed with thyroid cancer patient sera. Recovery studies utilized sera from 15 thyroid cancer patients, 4 Hashimoto's thyroiditis patients, and 1 Grave's disease patient. TgAb were also measured directly by an IRMA thyroglobulin antibody kit (Sanofi-Pasteur). RESULTS: Among the randomly selected 20 samples assayed in parallel studies, low recoveries (< 80%) were present in 2 samples by IRMA assay (Sanofi-Pasteur), 8 samples by FIA assay (Wallac-Delfia), and 10 samples by RIA assay (DPC). The majority of the low recovery samples contained high titers of TgAb. All three kits correlated well with the Mayo method. At the control-1 level (approximately 9.9 microgram/L by Delfia determination), both the IRMA kit (CV = 1.96%, n = 10) and FIA Kit (CV = 2.16%, n = 10) showed good within-run precision. CONCLUSIONS: In our hands, the IRMA Kit (Sanofi-Pasteur) demonstrated excellent precision, analyte recoveries, the least interference from TgAb, and good correlation with the Mayo Medical Laboratories procedure.

Autoantibodies↗
PubMed1994

Balloon or bougie for dilatation of benign esophageal stricture?

Ninety-three adult patients with benign esophageal stricture were randomized to receive balloon or bougie dilatation. Eighty-five patients were eligible for analysis and were followed prospectively for a year. Twenty-four patients required repeat dilatation within a year, but 50 patients completed a year's follow-up without further dilatation. The bougie group initially had a better symptomatic result, experiencing significantly less dysphagia at five months, although this difference had disappeared at one year. Eighteen patients in the balloon group required redilatation for symptoms compared with six in the bougie group. The bougie group had a significantly greater increase in their stricture diameter, and this was still present at one year after dilatation. There was no significant difference in safety or patient acceptability. Balloons are probably more costly to use than bougies. Bougie dilatation is to be preferred to balloon dilatation in adults except in special circumstances.

Aged↗
PubMed1990

Enema or Picolax as preparation for flexible sigmoidoscopy?

Two preparations for outpatient flexible sigmoidoscopy, Picolax and Klyx enemas, have been compared in a randomized controlled trial. There was no difference in efficacy between the two preparations, but patients preferred Picolax.

Adolescent↗
PubMed1988

Balloon or bougie for dilatation of benign oesophageal stricture? An interim report of a randomised controlled trial.

Seventy one patients with benign oesophageal strictures were randomised to receive balloon or bougie dilatation. Sixty five patients were eligible for analysis. At the end of five months the balloon group had significantly more dysphagia and the calibre of the strictures in the balloon group had narrowed by a greater degree. The methods were equally safe and acceptable to patients. While the choice of the method of dilatation depends on the individual patient's needs and operator experience, bougie dilatation is more effective in reducing dysphagia and maintaining stricture patency.

Adult↗
PubMed1987

The interaction of human T-lymphocytes and Entamoeba histolytica: killing of virulent amoebae by lectin-dependent lymphocytes.

Clinical and experimental studies indicate that following invasive disease due to Entamoeba histolytica, development of human cell-mediated immune mechanisms may provide protective immunity. Activated, human monocyte-derived macrophages in vitro can kill virulent axaenic amoebic trophozoites. This study describes the interaction of lectin-stimulated T-lymphocytes and E. histolytica trophozoites (virulent strain HM1-IMSS). Amoebae progressively killed unstimulated nonimmune T-lymphocytes over 18 h incubation with no effect on amoebic viability. T-lymphocytes, stimulated with phytohaemagglutinin (PHA), were progressively cytotoxic for virulent HMI amoebae over 18 h incubation, but were also reduced in viability themselves. Lymphocyte cytotoxicity for amoebae was absent if PHA was removed before or added only during the assay. PHA-stimulated T-lymphocytes killed amoebae at cell ratios of lymphocytes to amoebae as low as 50:1 and cytotoxicity was antibody-independent. PHA-stimulated T-lymphocytes, depleted of T8-bearing cells by complement-mediated lysis, were unable to kill amoebae. Adherence of PHA-stimulated T-lymphocytes to amoebae was greater than with unstimulated T-lymphocytes. Inhibition of the amoebic adherence lectin with N-acetyl-D-galactosamine decreased lymphocyte-amoebic adherence and resulted in increased lymphocyte amoebicidal activity and lymphocyte survival. Suspension of amoebae with or without adherent PHA-stimulated T-lymphocytes in a 10% dextran solution indicated that cytotoxicity was contact dependent. In summary, PHA-stimulated T-lymphocytes of the T8-phenotype can kill virulent axaenic E. histolytica trophozoites through a contact-dependent, antibody-independent mechanism.

Animals↗
PubMed1986

Comparison of corticosteroid therapy in the prevention of pelvic tissue reaction and adhesion formation.

The purpose of this research was to compare the effect of glucocorticoids administered in equivalent doses with respect to inflammatory response, fibrosis and adhesion formation. The animal model used was the Sprague-Dawley rat. The surgical technique involved incision and eversion of a portion of the proximal uterine horn. The glucocorticoids administered included methylprednisolone acetate (Medrol, The Upjohn Company), 6.25 mg/kg; hydrocortisone acetate (Hydrocortisone, Merck Sharp and Dohme), 25 mg/kg; betamethasone phosphate (Celestone Phosphate Injection, Schering Corp.), 1 mg/kg; and dexamethasone sodium phosphate (Hexadrol, Organon Pharmaceuticals), 1 mg/kg. These glucocorticoids were compared with a control solution of normal sodium chloride for injection (Abbott Laboratories), 0.5 mL/rat. Four weeks after the initial surgery, the uterine horns were histologically evaluated for inflammatory response, fibrosis and adhesion formation. Betamethasone phosphate produced a significant reduction (P less than 0.05) in fibrosis when compared with all other corticosteroid or control solutions. There was no statistically significant difference in the degree of inflammation or adhesion formation.

Adrenal Cortex Hormones↗
PubMedDate not supplied

An audit of the use of sphygmomanometers.

This survey assessed the measurement of blood pressure and use of sphygmomanometers by general practitioners in Humberside and Yorkshire in 1988. All registered practitioners were sent a questionnaire--1223 (58%) returned it completed. Their responses showed that 269 (22%) had an aneroid sphygmomanometer only, and that 120 (10%) had a random zero sphygmomanometer; 700 (57%) had special cuffs for obese patients, and 558 (46%) had special cuffs for children; 228 (23.5%) had never had their sphygmomanometers serviced ('never' corresponding to a mean time of 5.75 years), while 23% had patients who measured their own blood pressure. These findings suggest that the British Hypertension Society's recommendations on servicing and cuff use are not always applied. A formal audit of the use of different types of sphygmomanometer in general practice may encourage and improve accuracy and uniformity in blood pressure recording.

Blood Pressure Determination↗