Biomedical subjects

J G Heider

Publications and source records attributed to J G Heider.

2 recordsLinked to original sources

PubMed1978

The picomole determination of free and total cholesterol in cells in culture.

An enzymatic, fluorometric method for the determination of free and total cholesterol in cells in culture is presented. The method is simple, requiring one reagent that includes all of the enzymes and a second reagent that increases the pH, which enhances the fluorescence of the product. The method is based on the enzymatic hydrolysis of cholesteryl esters to free cholesterol, the oxidation of cholesterol with the liberation of hydrogen peroxide, and the reaction of this peroxide with a fluorogen to form a fluoresencet product in the presence of a peroxidase. It is rapid, in that free cholesterol can be read in 5 minutes and total cholesterol after 20 minutes. The precision of the method is greater than that obtained from gas-liquid chromatography.

Animals↗

PubMedDate not supplied

Biotransformation of mazindol. II. Absorption and excretion in the dog and man.

On administration of tritium-labeled mazindol (l) to man and the dog, absorption was slow but complete. Excretion was slow in both dog and man, but the dog did excrete the drug and/or metabolites faster than man. Blood levels of l in man after a single oral dose of a 2-mg tablet reached a peak value of 2.5 ng/ml, which remained essentially unchanged for at least 6 hr. After multiple doses of a 2-mg tablet t.i.d. for 4 days to subjects who had been receiving nonlabeled l, 2 mg t.i.d. for 6 months, the blood level reached a maximum concentration of 10.8 ng/ml, measured 2 hr after the last dose on day 4. This value is somewhat higher than predicted by the single-dose data, indicating that in man, mazindol does not induce its own metabolism.

Absorption↗