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J G Rateau

Publications and source records attributed to J G Rateau.

24 records · Page 2Linked to original sources
PubMedDate not supplied

[Cholestyramine adsorbs Escherichia coli and Vibrio cholerae toxins by way of ion exchange mechanism].

The use of cholestyramine in the treatment of enterocolitis is justified by its ability to fix the LT toxin of Vibrio cholerae and ETEC, the ST toxin of ETEC and the verotoxin of EHEC at the pH of intestinal fluid. To elucidate the nature of the ion-exchange mechanism in the binding of the toxins on the resin, we try to eluate the toxins with 0.1 M to 0.9 M NaCl solution at pH 7. Irrespective of their presence or absence in the different fractions, the biological activity of the toxins was assessed in cell culture and in the new-born mouse test. Desorption of the LT toxin from Vibrio cholerae is obtained with 0.6 M to 0.8 M of NaCl, with 0.3 M to 0.5 M of NaCl for the LT, 0.4 M to 0.5 M for the VT and 0.6 M for the TS toxins. The eluated toxins are biologically active; these facts demonstrate that the adsorption of toxin on cholestyramine result of a ion-exchange mechanism.

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[Ability of cholestyramine to bind Escherichia coli and Vibrio cholerae toxins].

Cholestyramine treatment of new born's infectious diarrhea has been shown to be effective. The study of in vitro binding of bacterial toxins from Vibrio Cholerae and from three strains of Escherichia coli suggest an ionic adsorption of the four toxins to this anion-exchange resin. This immediate binding is effective at the pH of intestinal fluid, therefore the protection of the enterocytes from the biological action of the toxins result of the toxins sequestering effect of the Cholestyramine.

Adsorption↗
PubMedDate not supplied

Experimental study, in the rat, on the protection of the gastric mucosa by the combination of an antacid and a montmorrillonite.

The stomach wall is protected by its own secretion of mucus. The superficial layer consist of a parietal film of acid mucus, adhering to the epithelium. The acid mucus reach its maximum viscosity at pH 5. We investigate, in the rat, the interaction between this mucus with a montmorillonite, a kind of clay, known to have a mucoprotective effect, and with the association of montmorillonite and an antiacid drug. Three hours after ingestion the clay particles saturated the parietal mucus, then, up to the sixth hour their abondance decrease. The parietal film of mucus is abundant until the sixth hour, this data confirm the protection of the gastric mucosa by this drugs association.

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