Biomedical subjects

J G Wheeler

Publications and source records attributed to J G Wheeler.

75 records · Page 5Linked to original sources

PubMedDate not supplied

Function of irradiated polymorphonuclear leukocytes obtained by buffy-coat centrifugation.

Several studies suggest that transfusion of polymorphonuclear leukocytes (PMNs) may be beneficial in the treatment of septic neonatal patients. Because of expense, donor availability, and the technical effort involved in obtaining PMNs by intermittent or continuous flow leukapheresis, buffy coat centrifugation of whole blood has been suggested as an alternative source. An in vitro study was performed to determine whether PMNs collected by this method have adequate oxidative and migratory function measured by chemiluminescence (CL) and chemotaxis under agarose (CT), respectively. Whole blood samples from six adult volunteers were drawn into citrate-phosphate-dextrose-adenine-one and stored at 4 degrees C for 0 to 48 hours. One-half of each sample was irradiated with 1500 rads. PMNs isolated from the buffy coat of these samples had greater than 80 percent normal CT and CL following 0 to 28 hours of storage in whole blood. Irradiation caused no depression in function. Units of whole blood yielded 1.11 +/- 0.40 X 10(9) PMNs per unit. This study indicates that transfusion of radiated PMNs obtained from stored whole blood that is less than 28 hours old is reasonable to use in studies involving PMN transfusions.

Adult↗

PubMedDate not supplied

Neutrophil storage pool depletion in septic, neutropenic neonates.

A prospective study was done to determine the incidence of neutrophil storage pool (NSP) depletion in clinically septic neonates with peripheral neutropenia (less than 1500/mm3). Infants with NSP depletion were then randomized in a controlled study of polymorphonuclear leukocyte transfusions. Bone marrow examinations were done in 13 patients, and NSP depletion was noted in 3 (23%) patients. All patients with no NSP depletion had peripheral neutrophil recovery and 8 of 10 survived. Complications of meningitis contributed to both deaths. Two of the three patients with NSP depletion died. Neither the initial severity of illness nor the degree of peripheral neutropenia were predictive either of NSP depletion or of mortality. Most neonates with severe peripheral neutropenia and clinical sepsis had peripheral neutrophil recovery and survived with conservative management. Those at high risk could be identified only by examination of the NSP. Only those patients with NSP depletion should be considered for controlled studies of polymorphonuclear leukocyte transfusions.

Agranulocytosis↗

PubMedDate not supplied

Atopic dermatitis and food hypersensitivity in children.

Atopic dermatitis is a common condition of early childhood. The association of atopic dermatitis and allergy has been studied for several years. Recent work has shown that food hypersensitivity does play a role as an exacerbating factor in some children with atopic dermatitis. This hypersensitivity will be limited typically to one or two food antigens. Children placed on appropriate elimination diets can be expected to have significant improvement in their clinical course.

Child↗