Biomedical subjects

J G Wu

Publications and source records attributed to J G Wu.

35 records · Page 2Linked to original sources

PubMed1998

Negative correlation between cyclophilin mRNA level in leukocytes of renal allograft recipients and ciclosporin concentration in whole blood.

AIM: To study mRNA level of cyclophilin in the white blood cells (WBC) of the renal allograft recipients (RAR) and its correlation with ciclosporin concentration in whole blood. METHODS: The cyclophilin mRNA levels and beta-actin as controls in the WBC of 47 RAR were measured by quantitative reverse transcription polymerase chain reaction. The blood ciclosporin assay utilized monoclonal antibody fluorescence polarization immunoassay. RESULTS: With the increase of ciclosporin concentration in whole blood (from 62 micrograms.L-1 to 678 micrograms.L-1), relative cyclophilin mRNA level in the WBC of RAR decreased nonlinearly (from 1.1 to 0.03, r = 0.8195). CONCLUSION: There was a negative correlation between the mRNA level of cyclophilin in the WBC of RAR and the ciclosporin concentration in whole blood.

Adolescent↗

PubMed1997

[Effect of acupoint irradiation with Q-wave millimeter microwave on peripheral white blood cells in post-operational treatment with chemotherapy in stomach and colorectal cancer patients].

OBJECTIVE: To explore the biological effect of Q-wave millimeter microwave (QWMM). METHODS: The QWMM was used to irradiate the acupoints Xuehai (Sp10) and Geshu (B17) in treating post-operational and chemotherapy treated stomach cancer and colorectal cancer patients. The effect of irradiation on chemotherapy affected peripheral white blood cells was observed. 62 cases (stomach cancer 42, colorectal cancer 20) in total were divided into two groups: group A, 21 cases (stomach cancer 15, colorectal cancer 6) the irradiation began 10 days after operation, and on the 16th day the chemotherapy combined with irradiation started. Group B had 41 cases (stomach cancer 27, colorectal cancer 14), in which the irradiation began immediately after the occurrence of chemotherapy induced peripheral WBC reduction, which persisted for at least 12 days. RESULTS: The effective rate for the group A and B was 85.7% (18/21) and 73.2% (30/41) respectively. The total effective rate of the two groups was 77.4% (48/62). The effective rate of group A was significantly higher than that of group B, P < 0.01. CONCLUSION: GWMM irradiation at acupoints could promote the hematopoietic function of bone marrow, and the irradiation performed 1 week before chemotherapy yielded even better protection on bone marrow.

Acupuncture Points↗

PubMed1995

[Study on risk factors of vaginitis in married women at reproductive ages in rural areas Luliang County, Yunnan Province].

Utilizing data from 2,287 married women at reproductive ages from Luliang county in Yunnan province, a study of prevalence and risk factors of vaginitis was conducted. It is found that the prevalence of vaginitis in Luliang county was 10.2%. The main risk factors selected by Logistic regression were occupation, contraceptive methods used currently, numbers of pregnancies, sexual intercourse per month, habit of washing lower body with dirty water during menses etc. The main risk factors through chi 2-test were age, occupation age of marriage, numbers of pregnancies, numbers of induced abortion, numbers of natural abortion, numbers of stillbirth, numbers of total failing pregnancies, contraception methods used currently, contraception methods ever used before, intercourse per month, wash lower body with dirty water during menses.

Adolescent↗

PubMed1993

Chronic tonsillitis and IgA nephropathy.

The authors studied the effect of tonsillectomy for IgA nephropathy and the relationship between chronic tonsillitis and IgA nephropathy. Forty-five patients with IgA nephropathy were treated by tonsillectomy in our department. The effective rate of hematuria type IgA nephropathy was 86.2% and that of non-hematuria type 62.5%. We also determined the immunoglobulin composition of the tonsilla tissue in 18 IgA nephropathy patients and compared it with that of 18 chronic tonsillitis patients. The immunoglobin level of the IgA nephropathy group was higher than that of the contrast group, indicating that the disorder of tonsilla immunity may be one of the pathogenic factors for IgA nephropathy. Since no specific method has been available for the treatment of IgA nephropathy, we use tonsillectomy as an effective procedure.

Adolescent↗

PubMed1992

Synthesis, characterization, and antiinflammatory activity of naproxen complexes with rare earth (III).

RE(III) complexes of Naproxen(HNap) have been synthesized and characterized by elemental analyses, conductance measurements, solubilities, thermal analysis, infrared, proton magnetic resonance, and electronic spectral data. The elemental analyses reveal the presence of 1:3 (metal:ligand) stoichiometry and the IR spectra suggest the carboxylate group of HNap functions, as a bridging ligand to coordinate to RE(III) ions. The electronic spectra recorded in solid exhibit only slight shifts in visible regions, on which beta, delta and b1/2 of covalent parameters have been calculated. Formalin-induced rat paw edema and croton oil-induced rat ear edema inflammatory models were chosen to examine the antiinflammatory activity of Nd(III) complex, which ascertained enhanced antiinflammatory activity relative to the ligand.

Animals↗

PubMed1990

Fibronectin in patients with chronic renal failure undergoing dialysis.

Fibronectin (FN) levels were determined in 64 cases with chronic renal failure (CRF), some of whom were undergoing dialysis. FN levels were 14.9 +/- 7.6 mg/dl in CRF (n = 20), 13.4 +/- 4.3 mg/dl in patients on continuous ambulatory peritoneal dialysis (CAPD) (n = 20) and 16.7 +/- 7.2 mg/dl in patients on hemodialysis (HD) (n = 24). All the levels were significantly lower than in normal subjects (23.1 +/- 4.6 mg/dl). Serum FN was compared with some nutritional indices. Positive correlations were found between serum FN and nitrogen balance (BN), serum prealbumin (PreA) and transferrin (Tf) in all the patients. With serum albumin (Alb), however, this correlation was only found in patients undergoing dialysis. Negative correlations were found between serum FN and the ratio of serum urea to serum creatinine (Surea/Scr) in CAPD and HD patients. In 10 CAPD patients, the low serum FN levels went up after increased protein intake. This indicates that it was the result of malnutrition due to decreased protein intake. Serum FN level reflects a negative BN earlier and better than serum PreA, Tf and Alb. It is a sensitive, reliable and simple index for judging the nutritional protein status and the effect of nutritional treatment in patients with CRF undergoing dialysis.

Adolescent↗

PubMed1990

A review of botulism in China.

The epidemiological characteristics and etiology of botulism in China, as well as the distribution of different types of Clostridium botulinum in China, are described. Through 1989, 15 provinces and autonomous regions reported the occurrence of botulism. There were 2861 cases involved in 745 outbreaks. Among the cases 421 died, with a case fatality of 14.7%. The main epidemiological characteristics of botulism in China are: (i) the major foods causing botulism are homemade fermented bean products which accounted for 62.6% of the cases; (ii) the incubation period is longer (3 h-54 days) than that described in the western literature (mostly 2-7 days); (iii) the peak occurrence is from February to May; (iv) the progression of symptoms and signs is slower than that of western cases. All types of C. botulinum, with the exception of type G, have been found in China. The distribution of various types of C. botulinum is significantly different between southern and northern China; this is related to the latitude and is correlated with the prevalence of this disease. Most of the botulism outbreaks occurred above 30 degrees north latitude in northern China and outbreaks rarely occurred below 30 degrees north latitude. Nationwide surveys showed that the average detection rate of C. botulinum spores in soil and foods in the northern parts of China was 14.8%, while it was only 2.5% in the south. C. botulinum types A, B, E, and F, which are involved in human botulism, were frequently found in the North, while types C and D, which are involved only in animal intoxication, were found more frequently in the south.

Botulism↗

PubMed1989

Sequence, transcription and translation of a late gene of the Autographa californica nuclear polyhedrosis virus encoding a 34.8K polypeptide.

A 1.4 kb region downstream of the DNA polymerase gene of Autographa californica nuclear polyhedrosis virus was sequenced. Two open reading frames (ORFs) were identified of 927 and 474 bases in length. The 927 base ORF encodes a 34.8K protein as determined by in vitro translation of both hybrid-selected RNA and RNA synthesized in vitro from a 927 base ORF template. The predicted amino acid sequence of the 34.8K polypeptide (p34.8) reveals a hydrophobic N terminus, two potential N-glycosylation sites, and potential sites for phosphorylation by casein kinase I and protein kinase C. The p34.8 gene has a strong codon usage bias which is strikingly different from that of the polyhedrin gene. The two 5' ends of the 927 base ORF transcripts initiate from an ATAAG sequence and a GTAAG sequence 11 and 87 bases upstream of the ATG codon respectively. A short upstream reading frame is present in the leader sequence of the longer RNA. The transcripts have multiple 3' ends; the most proximal endpoint correlates with a polyadenylation signal overlapping the translational termination codon of the 927 base ORF. Transcripts of the latter were not observed early in the infection cycle but appeared 6 h after infection and were maximally expressed at 12 to 24 h post-infection. The late nature of these transcripts was confirmed by their sensitivity to aphidicolin and cycloheximide, inhibitors of DNA replication and protein synthesis respectively. Attempts to construct viral mutants carrying a deletion of the p34.8 gene and fusion with the beta-galactosidase gene suggest that the former gene is essential for viral replication.

Amino Acid Sequence↗

PubMed1988

The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene.

The Autographa californica nuclear polyhedrosis virus (AcMNPV) DNA polymerase gene was identified with the aid of an oligonucleotide probe corresponding to an amino acid sequence conserved among viral DNA polymerases of other virus families. A 3.6-kb pair region of the AcMNPV DNA, from 39.5 to 42.5 map units (m.u.), was sequenced and an open reading frame (ORF) of 2994 bp was observed. From the first ATG of this ORF, a translation product of 984 amino acids (Mr 114,310) was predicted. Amino acid sequence similarities to other viral DNA polymerases were found. Transcription was analyzed by Northern RNA blot analysis and nuclease protection studies of RNA:DNA hybrids. The ORF is transcribed in the counterclockwise direction as a 3-kb RNA. Transcripts appear to initiate at two differently regulated sites (ca. -120 and -212 bp) upstream of the initiating ATG (+1,+2,+3) and to be polyadenylated at a single site near a signal (A2UA3) which overlaps the translational termination signal (UAA) at +2952. Transcripts were observed only during a narrow window between 2 to 8 hr postinfection (p.i.) with maximum expression between 4 and 6 hr p.i. Polymerase gene transcripts were observed in the presence of the protein synthesis inhibitor cycloheximide which also blocked the shut-off of these early transcripts. Aphidicolin, an inhibitor of both viral and host DNA polymerases, inhibited polymerase gene transcription suggesting a unique regulation involving DNA replication.

Amino Acid Sequence↗

PubMed1987