Biomedical subjects

J Gäbler

Publications and source records attributed to J Gäbler.

3 recordsLinked to original sources

PubMed1993

The influence of storage time on the nucleotide concentration of canine platelets.

Nucleotide concentrations of canine platelets during storage for 50 days were determined to find out the variation in the concentration of various nucleotides in canine platelets during storage at +4 degrees C for 50 days. Platelets of 3 dogs were stored in whole blood in PVC bags containing CPDA-1 stabilizer. The bags were kept at 4 degrees C for 50 days. Changes in the energy metabolism of the platelets during storage were determined by successive measurements of the following nucleotides: adenosine-diphosphate (ADP), adenosine-tri-phosphate (ATP) and guanosine-tri-phosphate (GTP). In preparation for the measurement of their nucleotide content, the platelets were isolated from the blood at regular intervals and their membranes destroyed by deep freezing, thawing, double sonification in ice and protein precipitation with perchloric acid. The concentration of nucleotides within the platelets was determined by "High Pressure Liquid Chromatography" (HPLC). ADP, ATP and GTP were identified in the peak areas of the HPLC using 3 different techniques: comparison of retention time of standard nucleotide solutions as control samples, enzyme tests and thin layer chromatography. Adenosine-mono-phosphate (AMP), adenosine, hypoxanthine and inosine mono 5'-phosphate (IMP) were not found in dog platelets. Platelet ATP concentration decreased significantly during 50 days of storage (p < 0.001), and was reduced to 24% of the concentration measured after 1 hour of storage on day 50. ADP concentration also dropped significantly during 50 days of storage (p < 0.001). On day 50 the average ADP concentration was reduced to about 34% of the concentration measured after 1 hour of storage.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMed1982

[The radiological and clinical features of Gardner's syndrome (author's transl)].

Gardner's syndrome, completely expressed, consists of a trio of familial polyposis of the colon, osteomas and mesenchymal tumours of the skin. Inheritence is autosomal dominant. In many patients with familial polyposis of the colon, only mesenchymal skin tumours or osteomas can be demonstrated. It is therefore possible that Gardner's syndrome and familial polyposis represent two extremities of a single disease which is characterised by marked variability in the expressivity of the gene. Gardner's syndrome has been considered a rare condition occurring in only about 8% of patients with familial polyposis. Amongst the 20 patients with colonic polyposis from eleven families, mesenchymal and/or osseous lesions were found in seventeen (85%). Osteomas of the mandible were shown particularly frequently by orthopantomography. Since polyposis of the colon tends to remain symptomless for many years, the finding of osteomas in the facial skeleton, or recurrent skin tumours in young patients, should lead to further investigation.

Adolescent↗

PubMedDate not supplied

[Early postoperative functional differences between total knee arthroplasties supplied with mobile-bearing platform or fixed-bearing system--an analysis of gait pattern].

AIM: The goal of this study was to analyze differences of gait patterns among patients with total knee arthroplasty supplied with mobile-bearing platform in contrast to patients supplied with fixed-bearing knee systems. METHOD: For that reason 17 patients with mobile-bearing knee systems and two groups of 15 patients each with fixed-bearing knee systems have been examined clinically, radiologically, and by means of gait analysis up to 6 months postoperatively. Additionally 20 knee-healthy control subjects have been gait-analyzed for comparison. RESULTS: There existed no different developments of gait characteristics between the groups of patients in the preoperative status. After the operation an increase of the ground reaction force of the operated leg was observed in all groups. The functional range of motion in the operated knee joint improved among patients postoperatively. Besides, in the group with the mobile-bearing knee a significantly stronger flection of the operated leg during the stance phase under load could be proven. Between the groups of patients the average values of the respective radiological alignments did not exhibit statistically significant differences. No severe complications have been observed. CONCLUSION: For the group of patients with fixed-bearing knee course samples have been registered, which approximate the reference values of healthy control subjects more strongly. The entire course sample is characterized by an increased range of motion of the opposite side and the adjacent joints. The parameters measurable in the gait analysis point to a better biomechanical situation after implantation of the mobile-bearing system.

Adult↗