[Latency of herpes simplex virus. Current knowledge].
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Animals↗
Biomedical subjects
J García-de-Lomas
Publications and source records attributed to J García-de-Lomas.
[Molecular epidemiological markers of Legionella pneumophila in the Valencian Community (II). Restriction enzyme profiles of chromosomal DNA].
Restriction enzyme profiles of chromosomal DNA obtained from 96 Legionella pneumophila strains isolated from environmental and clinical isolates and digested with Hind III have been analyzed. A method to obtain chromosomal DNA to be use in restriction studies and its electrophoretic analysis have been developed. Studying the fragments showing higher differences when comparing the profiles obtained, 5 subtypes are defined and correlated with the isolation areas. There are similar restriction profiles independent of the plasmid contents.
Biomarkers↗
[Molecular epidemiological markers of Legionella pneumophila in the Valencian Community (I). Plasma levels].
We have investigated the presence of plasmids in 96 Legionella pneumophila serogroup 1 strains isolated from clinical and environmental samples from the Valencia area. Our results show a higher incidence of a 83 MD plasmid present in clinical as well as in environmental isolates, and a 120 MD plasmid with lower incidence and observed exclusively in strains isolated from one area. The variability in plasmid detection from L. pneumophila and the effect on it in a hospital after disinfection treatment is considered.
Biomarkers↗
[Molecular epidemiological markers of Legionella pneumophila in the Valencian Community (III). Peptide profiles].
Electrophoretic profiles using soluble peptides obtained from Legionella pneumophila serogroup 1 have been studied to be used as epidemiological markers. Using 8.5% polyacrylamide gels (SDS-PAGE) a unique profile common to environmental and clinical isolates has been detected showing 2 differential bands: a 150 kD and a 230 kD band present in only one clinical isolate. In order to achieve better resolution in the peptide profile 10 to 20% gradient SDS-PAGE has been used, confirming the existence of a common profile and 2 subtypes as well as another profile present in a smaller number of strains.
Bacterial Proteins↗
Histopathology of experimental Aspergillus fumigatus keratitis.
Histopathological studies in rabbit's eyes, 7 and 14 days after intracorneal inoculation with 1 X 10(5) Aspergillus fumigatus conidia have been performed. Similar lesions were found in both periods with fungal hyphae in the anterior third of corneal stroma, round cell infiltration from the sclero-corneal edge and in the anterior chamber and, neovascularization. No lesions were found in the Descemet's membrane. Gomori silver-methenamine stain with hematoxiline-eosine counter-stain was found to be the most reliable stain to detect fungal presence in corneal stroma, and Masson's trichromic stain in the study of pathological changes in ocular elements.
Animals↗
In vitro interaction between ofloxacin and cefotaxime against gram-positive and gram-negative bacteria involved in serious infections.
The checkerboard method was used to determine the antimicrobial activity of the combination cefotaxime/ofloxacin against 217 bacterial isolates involved in serious infections. Synergy or partial synergy was observed against 19 of 34 (55.8%) Staphylococcus aureus methicillin-susceptible isolates, 4 of 47 (8.4%) Streptococcus pneumoniae isolates, 28 of 34 (82.2%) Escherichia coli isolates and 70 of 102 (68.5%) Pseudomonas aeruginosa isolates. Antagonism was not observed with any of the isolates examined.
Anti-Infective Agents↗
Meropenem permeation through the outer membrane of Pseudomonas aeruginosa can involve pathways other than the OprD porin channel.
The outer membrane protein (OMP) OprD is the major channel through which carbapenems permeate the outer membrane of Pseudomonas aeruginosa. In this study, we analyzed the OMP profiles of several P. aeruginosa clinical isolates showing diminished susceptibility to imipenem while remaining susceptible to meropenem. All these isolates lacked OprD or showed a reduced expression of this porin. Susceptibility to meropenem was thus independent of the level of OprD expression, indicating that the antimicrobial could be taken up via an alternative route. The level of expression of OprC (70 kD) was also unrelated to meropenem susceptibility. Nevertheless, OMPs OprF and OprE were expressed by all isolates, suggesting that in the absence of OprD, these porins might be involved in the permeation of meropenem.
Drug Resistance, Microbial↗
Susceptibility of Pseudomonas aeruginosa isolates to ceftazidime is unrelated to the expression of the outer membrane protein OprC.
Previously, it has been postulated that the porin OprC facilitates the diffusion of ceftazidime through the outer membrane of Pseudomonas aeruginosa. To further investigate this claim, the outer membrane protein (OMP) profiles of 22 ceftazidime-susceptible clinical isolates were analyzed. No correlation was found between MIC values and the level of expression of OprC. Further, OprC was either undetectable or expressed in reduced amounts in 12 isolates. In contrast, OprF and OprE were present in all isolates studied. This study suggests that OprC is dispensable for the permeation of ceftazidime through the outer membrane of P. aeruginosa.
Bacterial Outer Membrane Proteins↗