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J Georgoulakis

Publications and source records attributed to J Georgoulakis.

8 recordsLinked to original sources
PubMed2004

DNA ploidy as a prognostic factor in muscle invasive transitional cell carcinoma of the bladder.

Radical cystectomy represents the treatment of choice for muscle-infiltrative bladder carcinoma; however, about 50% of patients relapse and die from the disease. In the present study, the prognostic significance of the DNA ploidy in transitional cell carcinoma of the urinary bladder (TCCB) is analyzed. The study was carried out on 66 patients with TCCB who underwent radical cystectomy. DNA ploidy was determined by flow cytometry (FCM) on paraffin-embedded specimens, and the results were analyzed and correlated with the tumor malignancy grade and stage and the clinical course. Forty of the 66 tumors studied (63%) were aneuploid. Aneuploid status was correlated with higher tumor T stage (P < 0.001) and grade (P < 0.001). Median follow up was 68 months (range: 12-105). Median survival was significantly longer in patients with diploid tumors (> 60 vs 45 months, P < 0.001). All patients with diploid tumors were alive and free of bladder cancer during follow-up, in contrast to only 30% of patients with aneuploid tumors. DNA ploidy was an independent prognostic factor, as shown by multivariate analysis (P = 0.006). All patients with pT > or = 3b and diploid tumors were alive at the time of analysis as opposed to none with aneuploid tumors. The results of this study suggest that DNA ploidy can provide prognostic information on patients with muscle invasive carcinoma of the bladder and might represent a means of selection for postoperative management.

Adult↗
PubMed2002

DNA flow-cytometric, histological and hormonal analysis of sertoli cell only syndrome (SECOS).

Sertoli cell only syndrome (SECOS) was identified on histology in 21 cases (16,28%) among 129 testicular biopsies performed in our department for azoospermia over the last 5 years. In these patients history, clinical features, hormonal levels, and histological findings were analyzed. In addition DNA flow-cytometric analysis was performed and showed an almost complete absence of haploid cells. All patients presented with elevated serum FSH levels suggesting a Sertoli cell damage or reduced production of inhibin due to the absence of sermatogenic cells. An good correlation was found between histological findings and DNA histograms. In conclusion SECOS is a syndrome of unknown aetiology presenting in men with azoospermia. DNA flow-cytometric analysis is a reliable, rapid and easy method in the diagnosis of SECOS, and can replace histological examination.

Adult↗
PubMed1999

Outpatient PPS will create hospitals' greatest challenge. Part I.

Medicare's Outpatient Perspective Payment System is scheduled for implementation on July 1, 2000, and most providers will experience a reduction in revenue as a result. Ted Matson and Jim Georgoulakis of Ambulatory Care Advisory Group, Inc. provide an overview of this new reimbursement system and what it will mean for hospitals.

Aged↗
PubMed1998

Evaluation of cytological morphologic criteria and AgNOR expression in male breast lesions.

Fine needle aspiration (FNA) breast smears from 81 male patients have been examined in our laboratory between 1988 and 1994. The cytological criteria for diagnosing male breast lesions and the expression of nuclear organizer regions were evaluated. Of the 81 cases examined cytologically, 50 cases were proven cytologically and histologically to be inactive gynaecomastia, two cases showed florid gynaecomastia and there were 10 adenocarcinomas; in the 17 cases in which no cytological evidence of gynaecomastia or malignancy was found, the histological diagnosis was gynaecomastia in 13 and there was one case of mastopathy; in two cases suspicious of malignancy on cytology the histological examination proved to be florid gynaecomastia in one case and the other showed an adenocarcinoma. The absolute specificity of FNA in this study was 74.28%, the complete specificity 98.5%, the absolute sensitivity 90.9% and the complete sensitivity 100%. The overall accuracy was 97.5%, the positive predictive value 91.66% and the negative predictive value 98.5%. In all cases of male breast carcinoma, AgNOR mean value was > or = 3; thus, it appears that AgNOR mean value 3 could be used as a cut-off value between benign and malignant male breast lesions. Our experience suggests that FNA is an acceptable procedure for the investigation of male breast lesions.

Adenocarcinoma↗
PubMed1997

Deoxyribonucleic acid flow cytometry in the assessment of spermatogenesis.

PURPOSE: We compared deoxyribonucleic acid (DNA) flow cytometric analysis of testicular tissue to quantitative assessment of spermatogenesis. MATERIALS AND METHODS: We studied 35 infertile men with azoospermia or oligospermia. All patients underwent incisional testicular biopsies. DNA flow cytometric analysis was performed on each specimen to evaluate the ability of the method to quantify alterations in spermatogenesis. The results were compared to quantitative histological examination. At least 100 spermatic tubules were examined on each specimen and the number of spermatids per tubule was counted. All histological specimens were examined by the same pathologist. RESULTS: Of the 35 specimens analyzed with DNA flow cytometry 5 were normal, while the percentage of haploid cells (spermatids and spermatozoa) was decreased (hypospermatogenesis) in 14, complete maturation arrest was noted in 2 and almost complete absence of haploid cells was found in 14. Comparing the findings on histological examination with histograms, excellent correlation was noted in cases of the Sertoli-cell-only syndrome and complete maturation arrest, while 3 of 14 histograms with hypospermatogenesis demonstrated normal spermatogenesis on histological examination. Additionally 1 of 5 histograms with norma, spermatogenesis demonstrated hypospermatogenesis on histological examination. CONCLUSIONS: DNA flow cytometry of the testicular tissue seems to be an objective and quantified method that can be used to investigate spermatogenesis in infertile men. It is also less time-consuming than any histological examination, permits management decisions within 1.5 hours after biopsy and may replace testicular histopathological study. Flow cytometric diagnoses correlated well with histopathological findings.

Adult↗
PubMed1996

Deoxyribonucleic acid measurements in transitional cell carcinomas: comparison of flow and image cytometry techniques.

PURPOSE: We compared the results of deoxyribonucleic acid (DNA) ploidy determinations performed by flow cytometry and image cytometry in transitional cell carcinomas of the bladder. MATERIALS AND METHODS: In 81 cases of transitional cell carcinoma of the bladder DNA indexes were measured by flow cytometry of bladder washings and tumor tissue samples, and by image cytometry of imprints from tumor tissue samples. RESULTS: There was good correlation between bladder washings and tissue samples analyzed by flow cytometry in 68 cases but aneuploidy was missed with bladder washings in 13 (16%). There was also good correlation between flow and image cytometry in 75 cases (92.59%) regarding the detection of aneuploidy. There was agreement between detection of aneuploidy and DNA index in 45 cases (55.5%), while both methods detected an aneuploid population in 30 (37%) but there was disagreement regarding DNA index. Aneuploid populations were missed by flow cytometry in 6 cases (7.4%). Furthermore, in 10 cases peridiploid peaks were found on the image cytometry histograms, which were not visible on flow cytometry. However, it was not possible to assess accurately if these were true peridiploid populations. CONCLUSIONS: There is good overall correlation between DNA content measured by flow and image cytometry but image cytometry has the advantage of visual discrimination, permitting preferential selection and analysis of tumor cells. However, certain problems remain with image cytometry, particularly in the case of peridiploid peaks, which cannot be classified accurately as showing true peridiploid or right shifted diploid populations.

Carcinoma, Transitional Cell↗