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Biomedical subjects

J Glas

Publications and source records attributed to J Glas.

4 recordsLinked to original sources
PubMed1991

Susceptibility contrast imaging of cerebral blood volume: human experience.

Magnetic resonance (MR) can offer a unique window on the structure/function relationships in the brain, by utilizing the established link between tissue function, metabolism, and hemodynamics. This report focuses on recent applications of MR-based cerebral blood volume (CBV) imaging in humans. Our methodology uses high-speed "single-shot" or echo planar imaging techniques, which provide the necessary temporal resolution for mapping the rapid cerebral transit of contrast agents. These MR CBV mapping techniques have been used to study normal human brain task activation and in the clinical study of patients with brain tumors. In the latter, positron emission tomography imaging was used for functional metabolic and CBV correlation. Susceptibility contrast CBV imaging should allow us to improve our understanding of the relationship between the detailed physiology and morphology of the microvascular bed and functional attributes of the brain. These techniques can be applied to understanding fundamental questions of cognitive neuroscience and can aid in improving diagnostic sensitivity and specificity in various neuropathologies.

Brain↗
PubMed1989

Open wound drainage versus wound excision in treating the modern assault rifle wound.

Military dogma of the past 20 years preaches that excision of all injured tissue around the path of a penetrating projectile is essential in wound treatment. To find out whether excising injured muscle surrounding a bullet path benefits healing over and above the benefit provided by a simple release of tension by incision, two groups of 90 kg swine were shot in the hind leg with a replica of the AK-74 assault rifle projectile. One group was treated by excision of injured tissue around the projectile path; in the other group no tissue was excised. Both groups were given parenteral penicillin for 5 days, and simple gauze dressings were used to cover the wounds. No difference in healing time occurred; the wounds in both groups had closed, and no epithelial defect remained by 20 to 22 days. These results indicate that the simple extremity wound caused by the modern-generation assault rifle, provided with adequate open drainage and systemic penicillin, heals as rapidly when the body defense mechanisms handle the disrupted tissue as when an attempt is made to excise it surgically.

Animals↗
PubMed1979

Purification and characterization of cloacin DF13 receptor from Enterobacter cloacae and its interaction with cloacin DF13 in vitro.

Extraction of the crude cell envelope fraction of cloacin DF13-susceptible Enterobacter cloacae strain 02 with Triton X-100 and ethylenediaminetetraacetate solubilized an outer membrane fraction which neutralized the lethal activity of cloacin DF13. A similar fraction could not be isolated from strains known to be lacking functional cloacin DF13 receptors. On this basis the isolated outer membrane fraction was assumed to contain the specific cloacin DF13 receptor. The receptor was purified to homogeneity by acetone precipitation and affinity chromatography, using cloacin DF13 as a ligand. The purified receptor was identified as a protein which consisted of a single polypeptide chain with an apparent molecular weight of 90,000 and a preponderance of acidic amino acids (pI = 5.0). The interaction of equimolar amounts of purified receptor and cloacin DF13 in vitro resulted in a complete, irreversible neutralization of the lethal activity of the bacteriocin. This interaction showed a temperature optimum at 43 degrees C but was only slightly affected by variation of the pH between 5.0 and 8.5 or by increasing the ionic strength of the incubation buffer. The receptor had no neutralizing activity towards other bacteriocins, such as colicin E1 or colicin E3.

Bacterial Proteins↗