Biomedical subjects
Publications and source records attributed to J Gmiński.
The paper deals with the current state of knowledge on lipoxins. The structure, multiple biosynthesis pathways, possible mechanisms of action, biological activities and participation of lipoxins in human pathology are characterised.
The influence of silicon treatment on the levels of trace elements zinc (Zn), copper (Cu), and iron (Fe) in serum and tissues was studied in rats. The concentrations of silicon, iron, and zinc were estimated in samples of sera and tissues of rats receiving per os a soluble, inorganic silicon compound--sodium metasilicate nonahydrate (Na2SiO3.9H2O), dissolved in the drinking water. An increase of copper concentrations in liver and aortic walls in the experimental group was observed, with simultaneous reduction of zinc amounts in serum and all the tissue samples in the course of the experiment. The iron concentrations in the analyzed samples did not show any significant changes between both groups. The silicon levels in serum and in all the examined tissues were significantly higher in the tested group. The results provide evidence for the silicon interaction with copper and zinc, which could result in a number of metabolic process modifications, antiatheromatous activity among them.
The aim of this study was to investigate anti-elastin antibodies of the IgG and IgM types in sera of patients suffering from lung cancer, using the DOT immunobinding assay. We studied 96 pathological and 40 control sera. Anti-elastin antibodies were found to be present in 45% of patients with small cell lung cancer, 19% of subjects with adenocarcinoma and not-identified lung tumor and 15% of patients with squamous cell lung cancer. They circulated in 5% of control persons only. The highest values of their titers were observed in the advanced stages of disease. In 55% of anti-elastin antibody positive small cell lung cancer patients, antibodies were of the IgM type, suggesting the initial step of the autoimmunization to elastin.
The effect of elastin peptides (kappa-elastin) was investigated on murine fibroblasts. The data indicate that elastin peptides increase the activities of antioxidant enzymes detoxifying free radicals and increase the lipid peroxide concentration within the cell. These results suggest that the influence of elastin peptides on oxygen metabolism may be related to their activities in vivo following elastin degradation and can contribute to their role in the pathogenesis of atherosclerosis.
Markers of elastin metabolism were estimated in sera of children from families with a high risk of atherosclerosis (ATH). There was no statistically significant difference in the serum elastase-like activity between the groups studied. The concentration of elastin-derived peptides was statistically significantly elevated in the ATH group. Anti-elastin antibodies were found to be present in 73% of ATH children, while they circulated in 5% of control subjects only. Antibodies observed in the youngest ATH children were of the IgM type, suggesting the initial stage of the autoimmunization to elastin. The data obtained in this study may indicate an enhanced metabolism of elastin in ATH children.
1. Elastin peptides (kappa-elastin) prepared by alcoholic potassium hydroxide degradation of insoluble elastin were shown to increase the activities of antioxidant enzymes (SOD, CAT, GSH-Px) and the lipid peroxide concentration within fibroblasts. 2. The preincubation of cells with nifedipine (calcium channel antagonist) and trifluoperazine (calmodulin antagonist) caused the decrease in the activities of studied enzymes and the concentration of TBA-reactive products in fibroblasts stimulated with kappa-elastin. 3. The preincubation with ketotifen (antiallergic drug) has no effect on the activities of SOD, CAT, GSH-Px and the lipid peroxide concentration in stimulated cells. 4. These data suggest the possibilities of pharmacological modulation of the biological effects induced by elastin-derived peptides.
Elastin-derived peptides, kappa-elastin, prepared by chemical degradation of insoluble elastin from bovine ligamentum nuchae, were shown to increase the elastase-like activity in the culture medium and cell fractions in fibroblasts. Preincubation of cells with nifedipine (calcium channel blocker) and trifluoperazine (calmodulin antagonist) induced a decrease in the activities of the enzyme under study. These data suggest the possibility of pharmacological modulation of the biological effects induced by elastin-derived peptides.
Experimental immunization of rabbits with soluble porcine elastin was performed. An increase in histamine level in blood of immunized animals was observed.
Experimental immunization of rabbits with soluble porcine elastin was performed. A statistically significant decrease in the level of insoluble elastin in the aorta of the immunized animals was observed. Estimations of soluble collagen fractions showed the rise of both salt-soluble and acid-soluble collagen in the aorta of rabbits immunized with soluble porcine elastin.
Immunological and microbial factors may lead to the formation of atherosclerotic lesions. Cross immune reactions between aortic kappa-2-elastin and antisera against some antigens of Gram-negative bacteria--Salmonella and Escherichia coli--were studied using the dot-immunobinding assay. Positive results were obtained with antisera against Salmonella AO, BO, CO, EO, HM and Escherichia coli OK(A). Among immunological mechanisms leading to atherosclerosis cross immunoreactivity of aortic antigens with antimicrobial antibodies should be taken into consideration.
Experimental immunization of rabbits with soluble porcine elastin was performed. Elastase-like activities in plasma and the aorta were assayed using succinyl trialanine p-nitroanilide. A statistically significant increase in the elastase-like activity in plasma of the immunized animals was observed. Estimations of the elastase-like activity in the rabbit aorta showed the rise of analyzed parameters in the experimental group.
Anti-elastin antibodies which seem to play an important role in atherogenesis were studied in serum of rabbits fed with a cholesterol-rich diet using the dot-immunobinding assay. These antibodies appeared only in rabbits of the experimental group after the sixth week of the experiment and a progressive rise in their titer was observed. The concentration of total serum cholesterol and the elastase-like activity in aorta which were determined additionally showed a statistically significant increase of both analyzed parameters in animals from the experimental group when compared to the controls. Anti-elastin antibodies seem to reflect an increased elastin turnover during the induction of experimental atherosclerosis.
Immunological factors seem to play an important role in the pathogenesis of atherosclerosis, which is a multifactorial chronic disease. It was demonstrated on animals that antibodies, immune complexes and cells may be the reason why the immunological damage in the vascular wall appears and may lead in consequence to the development of atherosclerotic changes. Immunological mechanisms of atherosclerosis are discussed.
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