PubMed Health⌕ Search

Biomedical subjects

J Gorrell

Publications and source records attributed to J Gorrell.

8 recordsLinked to original sources
PubMed1992

Quantitation of E. coli protein impurities in recombinant human interferon-gamma.

A multiple antigen ELISA for E. coli proteins (ECPs) that may be present in purified recombinant human interferon-gamma (rIFN-gamma) was developed. SDS-PAGE and Western blotting analyses showed that the assay antibodies reacted with a wide spectrum of ECPs in the standard and with ECPs in a production run. In spike recovery studies, rIFN-gamma at concentrations of 0.05 mg/mL and higher augmented the immunoreactivity of the ECPs in the standard curve (1.3-40.0 ng ECPs/mL) by approx 50%. To determine ECP content in purified rIFN-gamma, 0.2 mg/mL of rIFN-gamma was added to the standard curve diluent to compensate for enhanced immunoreactivity. The assay was precise (interassay precision of ECP controls < or = 4.1 %CV) and accurate with recoveries of 111-115% of expected for ECPs (15-40 ng/mL) spiked into purified rIFN-gamma (1 mg/mL). Linearity of dilution for ECPs spiked into rIFN-gamma was obtained (r = 0.999). Moreover, linearity of dilution was obtained for ECPs in "in-process" samples, demonstrating the required condition of antibody excess for this type of multiple antigen ELISA. ECPs were not detectable in several purified lots of rIFN-gamma. Therefore, these lots contained < 1.3 ppm ECPs.

Antibodies, Monoclonal↗
PubMed1991

Uterine arteriovenous malformations: primary treatment with therapeutic embolization.

Uterine arteriovenous malformations are rare lesions that can cause massive vaginal bleeding and spontaneous abortions. The majority are either congenital or related to gestational trophoblastic disease. Hysterectomy has been the treatment with symptomatic uterine arteriovenous malformations by means of transcatheter embolization; three of the lesions were related to gestational trophoblastic disease and one was congenital. All were supplied by the uterine arteries that were subselectively embolized with particulate material. This resulted in prompt cessation of uterine hemorrhage. There were no complications, and one pregnancy was achieved. The three other women have normal menstruation and no recurrence of bleeding at follow-up 10-48 months after treatment. On the basis of this experience and that of others, embolotherapy should be the treatment of first choice in these lesions, as it appears safe and effective. Additionally, uterine function is preserved in patients who are usually reproductively active.

Adolescent↗
PubMed1990

A successful partnership.

Physicians considering partnership agreements should follow a well-designed plan for recruitment, interviewing, and negotiation. This process can ensure future success of the partnership, and prevent legal difficulties.

Partnership Practice↗
PubMed1987

Spatial role-taking ability among bilingual and monolingual kindergarten children.

Fifty-seven children enrolled in a bilingual Spanish kindergarten program, assigned to appropriate language and age-related groups, were shown a graduated sequence of increasingly complex arrangements of multicolored blocks and were asked to judge how the original arrangement would look from the opposite and the side perspectives. A series of 2(younger vs. older) X 3(Spanish Monolingual vs. English Monolingual vs. Spanish-English Bilingual) ANOVAs for each of the types of responses (correct, incorrect, egocentric) showed a significant main effect for age on the incorrect answers. No differences associated with egocentrism were obtained. There was no relationship between age and success with simple and complex spatial tasks. As opposed to other studies that suggest certain cognitive advantages for young bilingual children, this study indicates no perceptible differences associated with being monolingual or bilingual at the ages of 5 and 6 for spatial tasks.

Child↗