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Biomedical subjects

J Goudswaard

Publications and source records attributed to J Goudswaard.

At least 19 recordsLinked to original sources
PubMed1991

[Good Laboratory Practice (GPL) and quality control in Dutch laboratories].

A review of the origin of GLP (Good Laboratory Practice) and ISO (International Standard Organisation) directives is followed by a number of definitions of concepts such as quality, guarantees of quality, quality systems, etc. by laboratories (NEN 2653). These requirements are discussed in the paper. Certification is one of the guarantees of quality assessment by laboratories. Certification of laboratories is carried out by STERLAB (Laboratory Accreditation Board of The Netherlands) or the CCKL (National Coordination Committee for Quality Assurance for Health Care Laboratories in The Netherlands). In addition to certification, laboratories in the Netherlands are extremely active as regards external quality control (QC). QC is carried out by the various occupational groups. The paper finally closes with a discussion of future developments regarding quality control and certification in medical and veterinary diagnostic laboratories.

Animal Welfare↗
PubMed1979

Antibodies against purified pigeon IgA in pigeon breeders' disease.

It is demonstrated that previously described PDF1-A antigens from pigeon dropping extract contain pigeon IgA. The sera of patients with pigeon breeders' disease contain precipitating antibodies against pigeon IgA, in contrast to the sera of pigeon breeders suffering from unrelated forms of pulmonary dysfunction. The degree of complement consumption by PDF1A antigens, pigeon serum, and pigeon IgA turned out to be correlated. No correlation was found between precipitating anti-pigeon IgA antibodies and complement consumption by pigeon IgA in patients' sera.

Alveolitis, Extrinsic Allergic↗
PubMed1978

Quantitation of lysozyme in the urine of the dog for the diagnosis of proximal tubular damage in renal disease.

The serum and urine LZM levels were determined with the lysoplate assay in 26 normal experimental dogs and in 21 dogs with various renal diseases. In the dogs with decreased glomerular filtration the serum LZM levels were higher than in normal dogs (less than or equal to 1.3 mg/l). No LZM could be demonstrated in the urine of normal dogs. The presence of LZM in urine was associated with proximal tubular damage, which indicates that LZM-uria can be used as an index of renal tubular damage.

Animals↗
PubMed1978

Lysozyme and its presence in bovine milk and serum.

The literature on the presence of lysozyme in various biological fluids in physiological and pathological conditions is reviewed. Preliminary results of lysozyme estimations in bovine milk show that lysozyme levels are definitely higher in colostrum and mastitis milk than in normal milk. The biological role of the enzyme in the milk still has to be defined. On the other hand, lysozyme may interfere with microbiological screening techniques for penicillin in cow's milk; the lytic effect of purified human lysozyme on B. stearothermophilus var. calidolactis was demonstrated microscopically.

Animals↗
PubMed1978

New immunofluorescent antibody test for diagnosis of candidiasis using macrophages-engulfed antigens as substrate.

A new method for detection of antibodies to polysaccharidic antigens of Candida albicans is described. The method is based on the selective uptake of carbohydrate-rich antigens by mouse peritoneal macrophages, the antibodies being revealed by indirect immunofluorescence after incubation of fixed, antigen-containing macrophages with sera (MU/IFA test). This test shows greater sensitivity than counterimmunoelectrophoresis individuals. The combination of CIE using cytoplasmic antigens and MU/IFA using surface antigens and MU/IFA using surface antigens is useful in a considerable majority of cases for diagnosis of clinical infection.

Animals↗
PubMed1978

Protein A reactivity of various mammalian immunoglobulins.

Serum samples and immunoglobulin fractions of eight mammalian species were applied to a Sepharose--protein A column. As with the human immunoglobulin subclasses IgG1, IgG2 and IgG4, all examined IgG classes and subclasses were bound to a greater or lesser extent to protein A. However, the binding of IgG1 of ruminants was very poor. Polyclonal IgM and IgA of the pig, the dog and the cat may be separated in protein A reactive and protein A non-reactive fractions. In addition, monoclonal canine IgM and IgA partially reacted with protein A. In combination with methods such as ammonium sulphate precipitation, ion exchange chromatography and gel-filtration, affinity chromatography with protein A is recommended for the rapid purification of certain Ig (sub)classes of a number of mammalian species.

Animals↗
PubMed1978

Pigeon IgA: a major antigen in pigeon breeder's disease.

Pigeon IgA has been isolated from crop-milk and an antiserum was produced. Extracts of the dust in pigeon coops contained detectable amounts of pigeon IgA, but not IgG. The sera of patients with pigeon breeder's disease contained precipitating antibody against pigeon IgA.

Alveolitis, Extrinsic Allergic↗
PubMed1978

Skin grafting and graft rejection in goats.

As part of a study to define the major histocompatibility complex of the goat and to show certain aspects of its biological function, a skin-grafting technique is described which might also be applicable for similar studies in other ruminant species.

Animals↗
PubMed1978

Quantification of turkey immunoglobulins IgA, IgM and IgG in serum and secretions.

The immunoglobulins IgA, IgM and IgG of the turkey were quantitated in individual serum samples as well as in pooled sera. The variability of Ig levels in normal, healthy birds was quite high: IgA: mean value 0.633 mg/ml (4.0 -x - 2.5 -x); IgM: mean value 4.37 mg/ml (0.5 -x - 1.4 -x) and IgG: mean value 8.92 mg/ml (0.6 -x - 1.7 -x). Immunoglobulin levels in egg-yolk, egg-white, bile and some intraocular tissues were quantitated as well. An interesting finding was, that the forementioned variability was by far not so high with respect to IgG levels in 20 egg-yolk samples: mean value 5.1 mg/ml (0.86 x- - 1.17 -x). Though IgG and IgM could be detected in pooled turkey bile, IgA predominated in this secretion. In aqueous humor, iris tissue and vitreous body only IgG could be detected.

Animals↗
PubMed1977

[Immunoglogulins in swine. (A) A review supplemented by a report on findings in personal studies (author's transl)].

the literature is reviewed and the importance of studying immunoglobulins in farm animals, particularly swine, is stressed. Possible methods of clinical immunological investigation in pigs are suggested. The literature as well as personal studies show that the immunoglobulin titre in the serum of piglets continues to be very low over a prolonged period from the seventh day post partum. This incomplete state of immunity should be taken into account on weaning piglets.

Animals↗
PubMed1977

[Immunoglobulins in swine. (B) Immunohistological studies on the localization of cells containing immunoglobulin in the tissues of the small intestine and spleen (author's transl)].

Antisera to porcine immunoglobulins, conjugated with flurescein isothiocyanate, were used in studies on the localization of cells containing immunoglobulin in the tissues of the intestine and spleen in pigs. Cells containing IgM and IgA were found to be present in the lamina propria of the intestinal mucosa, the villous membrane showing fluorescence due to liberated immunoglobulins. Cells containing IgG and IgM were detected in the marginal areas of the splenic lymph nodes but could not be definitely identified as mature plasma cells.

Animals↗