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Biomedical subjects

J H Anglin

Publications and source records attributed to J H Anglin.

15 recordsLinked to original sources
PubMed1979

Localization of specific carbohydrate configurations in human skin using fluorescein-labelled lectins.

The binding of three fluorescein-labelled lectins by normal human skin has been studied. The patterns of bound fluorescence seen were characteristic for each lectin, indicating that saccharide residues were available for lectin localization in the skin. Concanavalin A (glucose and mannose specific) and ricin 120 (galactose specific) presented similar patterns of localization of fluorescence in the epidermis and dermis. Both lectins presented a continuous band at the dermo--epidermal junction, but with concanavalin A the band was broader, while with ricin 120 the junctional band was thinner and more closely associated with the epidermal interface. With the peanut lectin (galactose specific) fluorescence outlined the periphery of the keratinocytes in the upper Malpighian and granular cell layers only; no junctional band was seen and the dermal appendages and collagen did not fluoresce. The stratum corneum did not fluoresce with any of the lectins studied.

Antibody Specificity↗
PubMed1978

Antigen shedding by human breast-cancer cells in vitro and in vivo.

Human breast cancer cells were adapted to chemically defined medium in order to recover naturally shed glycoproteins. Sephadex G-150 chromatography of these glycoproteins revealed 1 major and 2 minor peaks. When the cells were grown in the presence of 3H-leucine, the radioactive shed proteins had a Sephadex profile identical to the unlabelled shed glycoproteins. PAGE analysis of these proteins showed 5 major bands. Antigenically similar proteins were found in the serum of female nude mice bearing BOT-2 tumours, but not in controls.

Animals↗
PubMed1978

Cell-surface antigens from human breast tumor cells.

Biochemical and immunologic studies on breast cancer with the use of cells from a human ductal cell carcinoma, BOT-2, were initiated. Antigens were extracted from the cells by mild sonication and purified by gel filtration chromatography. Only one of the three peaks from gel filtration chromatography reacted with antiserum prepared against whole BOT-2 cells. Analysis by polyacrylamide gel electrophoresis of the BOT-2 cell extract revealed many protein bands, whereas analysis of the antibody-reactive peak after gel filtration chromatography revealed fewer protein bands. Immunologic tests to identify human serum antibodies against BOT-2 cells or cell extracts were performed by fixed cell immunofluorescence, living cell membrane immunofluorescence, and indirect hemagglutination. Depending on the test, the sera from women with diagnosed, untreated mammary cancer were positive in 45--80% of the cases, whereas the sera from women without apparent breast diseases (controls) were positive in only 5--10% of the cases. The results suggested that the antigens from the BOT-2 cells will be useful in understanding the processes involved in human mammary neoplasia.

Antibodies, Neoplasm↗