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Biomedical subjects

J H Berg

Publications and source records attributed to J H Berg.

At least 19 recordsLinked to original sources

Effects of argon laser irradiation and acidulated phosphate fluoride on root caries.

PURPOSE: To determine the effects of argon laser irradiation (ArI) and topical acidulated phosphate fluoride (APF) treatment on artificial caries formation in root surfaces. MATERIALS AND METHODS: After soft tissue debridement and fluoride-free prophylaxis, the teeth were divided into quarters and acid-resistant varnish was applied, leaving windows of sound root surface exposed. Each tooth received four separate treatments: (1) Control-mesiobuccal quarter; (2) ArI only--mesiolingual quarter; (3) ArI followed by APF- distobuccal quarter; (4) APF followed by ArI-distolingual quarter. ArI was for 10 seconds at 2W (100J/cm2) and APF treatment was for 4 minutes. After artificial lesion formation, sections were prepared and evaluated with polarized light. Mean lesion depths were determined and compared (ANOVA & DMR for a paired design). RESULTS: Mean lesion depths were: 347 +/- 41 microns--controls; 263 +/- 32 microns--ArI only; 158 +/- 21 microns--ArI followed by APF; and 149 +/- 17 microns -APF followed by ArI. Lesion depths were significantly different (P < 0.05) between the control group and each treatment group, and between ArI only group and either combined APF and ArI groups. No lesion depth difference (P > 0.05) was present between the combined APF and ArI groups. Argon laser irradiation significantly enhanced the resistance of root surfaces to demineralization. Combination of APF treatment with argon laser irradiation provided added protection against a constant artificial caries attack.

Acidulated Phosphate Fluoride↗

Acidulated phosphate fluoride treatment and formation of caries-like lesions in enamel: effect of application time.

The aim of this study was to evaluate the effect of acidulated phosphate fluoride (APF) application time (1 and 4 minutes) on caries-like lesion formation in enamel. Following a fluoride-free prophylaxis, the specimens (10 extracted human molars) were divided into tooth quarters and assigned to treatment groups: 1) Distobuccal and Distolingual Quarters--1 minute APF; 2) Mesiobuccal and Mesiolingual Quarters--4 minute APF. Acid-resistant varnish was applied to the tooth quarters, leaving sound enamel windows exposed on buccal and lingual surfaces. Following treatment, the APF gel (Oral-B Minute Gel) was removed by air-water spray rinsing, followed by a 24-hour water rinse. Sound enamel windows protected from APF treatment by acid-resistant varnish were created adjacent to APF-treated sound enamel windows and served as paired controls. Lesions were created in APF-treated and paired control sound enamel windows with an acidified gel. After lesion formation, sections were obtained and imbibed with water for polarized light study. Body of the lesion depths were measured and compared statistically. Mean body of the lesion depths were: 186 +/- 28 microns for control lesions, 117 +/- 12 microns for APF-1min; and 111 +/- 14 microns for APF-4min. Significant differences (p < 0.05) were found between paired control and APF groups. No difference (p > 0.05) was found between APF-1min and APF-4min groups. APF treatment prior to lesion formation resulted in a significant reduction in lesion depth, regardless of whether a 1 or 4 minute APF treatment period was used. The 1 minute APF treatment provided the same degree of caries protection as the longer treatment period.

Acidulated Phosphate Fluoride↗

Enamel caries initiation and progression following low fluence (energy) argon laser and fluoride treatment.

The aim of this study was to evaluate the effect of low fluence argon laser (AL) and acidulated phosphate fluoride (APF) treatment on caries initiation (CI) and progression (CP) in human enamel. Twenty caries-free molars were divided into tooth quarters. Tooth quarters from each specimen were assigned to one of four groups: 1) Control; 2) AL Only; 3) AL before APF; 4) APF before AL. AL was at 0.25 watts for 10 seconds (12.0 +/- 0.5 J/cm2). APF treatment was with a 1.23% gel for 4 minutes. Lesions were created in two treated, sound enamel windows per tooth quarter with an acidified gel. After CI and CP, sections were obtained and imbibed with water for polarized light study. Mean body of the lesion (BL) depths were determined and compared among groups (ANOVA & DMR). After CI, BL depths were: 189 +/- 29 micrometers for Control, 133 +/- 23 micrometers for AL only; 91 +/- 17 micrometers for AL before APF; and 83 +/- 14 micrometers for APF before AL. After CP, BL depths were: 321 +/- 43 micrometers for Control, 206 +/- 35 micrometers for AL only; 118 +/- 21 micrometers for AL before APF; and 114 +/- 19 micrometers for APF before AL. After CI and CP, argon laser irradiation alone resulted in significant reductions in lesion depth when compared with controls (p<0.05). APF treatment before or after argon laser exposure resulted in a significant reduction in lesion depth when compared with AL alone or control groups (p<0.05). Caries initiation and progression in vitro are affected to a significant extent when low fluence (energy) argon laser irradiation of sound enamel alone or in conjunction with APF treatment is done. This caries-protective effect occurs at an argon laser fluence (energy) that is capable of polymerizing visible light-cured resins.

Acidulated Phosphate Fluoride↗

The effects of vinblastine in assessment of the influence of age on proliferative activity of murine palate and footpad epithelium.

Data concerning changes in the rate of cell proliferation of stratified epithelia with increasing age are conflicting. In the present study young (3-month-old) and old (22-month-old) C57Bl/6NNia male mice were injected intraperitoneally with 2, 3, 4 or 8 mg vinblastine sulfate/kg body weight and killed after 1.5, 3, 4.5 or 6 h. The number of arrested metaphase figures per 1000 basal cells was counted in histological sections. Data were analysed using a multivariate analysis of variance. There was a significant difference between the accumulation of mitotic figures in footpad epidermis and palate epithelium and both tissues contained an increased number of mitotic figures with increasing periods of accumulation at all dose levels. In the footpad epidermis neither the age of the animal nor the dose of vinblastine had a significant effect on the number of mitotic figures. In contrast, for palate epithelium the accumulation of mitotic figures was significantly less in the old mice compared with the young mice and at a dose of vinblastine of 2 mg/kg compared with the higher doses. There was a statistically significant interaction between the dose of vinblastine and its period of action. It was concluded that the different tissues manifest a differential sensitivity to vinblastine and that only palate epithelium showed a significant reduction in proliferative activity with age.

Aging↗

Comparison of patient-perceived postcementation sensitivity with glass-ionomer and zinc phosphate cements.

Numerous investigators have reported patients' postcementation sensitivity with glass-ionomer luting agents. This information has been predominantly anecdotal and unsupported by data. This paper reports on the actual perceptions of patients who had restorations cemented with both glass-ionomer and zinc phosphate luting agents. Forty-five patients were randomly selected and received two cast restorations, one cemented with glass-ionomer cement and the other with zinc phosphate cement, also by random assignment. After 1 week, patients returned to complete a self-administered evaluation of perceived sensitivity. Neither luting agent resulted in greater sensitivity when used according to its manufacturer's instructions.

Crowns↗

Caries-like lesion initiation and progression around laser-cured sealants.

This study evaluated and compared the effects of argon laser and visible light polymerization of a pit and fissure sealant material on caries-like lesion initiation and progression. Following the lesion initiation period, the primary surface lesion depth was significantly less (P < 0.05) for the argon laser-cured group (97 microns) when compared with that for the visible light-cured group (151 microns). Wall lesion occurrence was 5% for both treatment groups. Following lesion progression, the primary surface lesion depth was significantly less (P < 0.05) for the argon laser-cured group (129 microns) when compared with that for the visible light-cured group (232 microns). Wall lesion occurrence was 15% and 5% for visible light-cured and argon laser-cured groups, respectively (P < 0.05). Argon laser-curing of sealant material may enhance the caries resistance of the enamel forming the enamel-resin interface and result in a reduction in caries formation adjacent to sealants.

Argon↗

Fluoride supplementation. A survey of pediatricians and pediatric dentists.

OBJECTIVE: To determine the protocol and use of prescriptions of fluoride supplementation by primary care pediatricians and pediatric dentists in the Houston (Tex) area. DESIGN: Survey mailed to all primary care pediatricians and pediatric dentists listed in the Yellow Pages of the Greater Houston telephone directory. PARTICIPANTS: 153 pediatricians and 47 pediatric dentists. MAIN RESULTS: Ninety-six percent of the participants prescribed fluoride supplements. Fifty-one percent of the pediatricians and 61% of the dentists considered that the fluoride content of the water was important. Seventy percent of the pediatricians and 30% of the dentists discontinued the use of supplements by age 7 to 10 years. CONCLUSIONS: Pediatricians and pediatric dentists should consider the need for water analysis prior to supplementation and should continue the use of fluoride supplements until 16 years of age.

Adolescent↗

Microleakage of interim endodontic filling materials.

This study compared four interim endodontic filling materials for microleakage. Forty extracted premolars were prepared for first appointment endodontic therapy. Each tooth was filled with one of four materials, thermocycled, placed in basic fuchsin dye, sectioned, and photographed. Ketac-Silver showed virtually no leakage; IRM exhibited leakage ranging from minimal to the depth of the dentin; Term had leakage ranging from slight to the depth of the pulp; gutta-percha exhibited leakage in the various samples from deep dentin penetration to pulp chamber penetration. Ketac-Silver demonstrated superior sealing qualities compared to the other three materials. The gutt-percha was remarkably inferior to the other materials.

Dental Leakage↗

Class II glass ionomer/silver cermet restorations and their effect on interproximal growth of mutans streptococci.

The release of fluoride from glass ionomer materials is one of the most important features of this newly implemented material, and the remineralization effects of this phenomenon have been documented (Hicks and Silverstone 1986). This paper examines the effects of glass ionomer/silver cermet restorations on the plaque levels of interproximal mutans streptococci. Fifteen patients with Class II lesions in primary molars were selected for study. Interproximal plaque samples were obtained from each of the lesion sites and from one caries-free site approximal to a primary molar. One lesion was restored with composite resin to serve as a treated control to the glass ionomer/silver cermet (Ketac Silver, ESPE/Premier Sales Corp., Norristown, Pennsylvania) test site. A sound (unaltered) interproximal site served as the untreated control site. Plaque samples were collected before and at one week, one month, and three months post-treatment. Samples were serially diluted to enable colony counts of mutans streptococci. One week post-treatment counts showed that the glass ionomer/silver cermet restorations significantly reduced (P less than 0.05) the approximal plaque levels of mutans streptococci. Conversely, the untreated and treated control sites did not exhibit reductions in approximal plaque levels of mutans streptococci. These results indicate that glass ionomer restorations may be inhibitory to the growth of mutans streptococci in dental plaque approximal to this restorative material in the primary dentition.

Cermet Cements↗

Dental management of children with cancer.

Dental management of pediatric oncology patients is an important component of comprehensive supportive care. Oral complications associated with tumor directed treatment may be a direct result of the stomatotoxic effects of cancer therapy or the indirect effects of myelosuppression. These complications may be immediate or long-term. Appropriate dental supportive care has been shown to minimize the morbidity and potential mortality associated with these complications.

Antineoplastic Agents↗