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J H Booth

Publications and source records attributed to J H Booth.

6 recordsLinked to original sources
PubMed1995

Expression of two mucin antigens in cultured human ovarian surface epithelium: influence of a family history of ovarian cancer.

OBJECTIVE: The genetic changes in hereditary ovarian cancer syndromes suggest that the phenotype of ovarian surface epithelium in women with familial ovarian cancer might be altered. To test this hypothesis, we compared two tumor markers, CA 125 and 2G3, in cultures of overtly normal epithelium from patients with and without familial ovarian cancer. STUDY DESIGN: Surface epithelia from 18 patients with no family history of ovarian cancer, five with family histories that were insufficient to be classified as familial, and seven with strong family histories were examined by immunofluorescence, immunocytochemistry, and radioimmunoassay. The influence of cell density, morphologic features, propagation in culture, and immortalization with SV40 on the expression of the markers was investigated. RESULTS: CA 125 occurred in cells with epithelial rather than atypical morphologic features. In cultures with no family history and minor family history, CA 125 was present in up to 45% cells in passage 1 but in only < 5% cells in 14 of 16 cultures by passages 3 to 4. In contrast, nine of 10 cultures with family history retained > 5% CA 125-positive cells in passages 3 to 4. This prolonged presence of CA 125 correlated with a persisting epithelial phenotype, whereas most cells with no family history and minor family history became atypical by passage 3. Immortalization eliminated CA 125 in all three types of cells. 2G3 bound to few cells in low passage, independent of family history and morphologic features. The proportion of 2G3-expressing cells increased significantly with immortalization in all cultures, independent of family history. Ovarian carcinoma lines expressed both markers. CONCLUSION: In cultures of ovarian surface epithelium 2G3 expression increases with immortalization, whereas CA 125 is lost with immortalization but correlates with epithelial cell morphologic features and with family history. The results suggest that there may be phenotypic changes in overtly normal ovarian surface epithelium of women with family histories of ovarian cancer.

Antigens, Neoplasm↗
PubMed1994

In vitro interactions of neomycin sulfate, bacitracin, and polymyxin B sulfate.

BACKGROUND: Neomycin sulfate, bacitracin, and polymyxin B sulfate have been combined in topical preparations to provide a complementary antimicrobial spectrum for the prevention of minor wound infections. The advisability of the inclusion of neomycin sulfate has been questioned since it may cause contact sensitization. METHODS: To assess the value of neomycin, microdilution checkerboard titrations were used to determine the in vitro interactions between two-antibiotic and three-antibiotic combinations against reference strains of bacteria commonly associated with wound infections. Using Fractional Inhibitory Concentration (FIC) indices (< 0.5 indicates synergism with two-drug combinations), the combination of neomycin/bacitracin was synergistic for both S. aureus and Ps. aeruginosa; neomycin/polymyxin B was synergistic for E. faecalis, and the bacitracin/polymyxin B combination was synergistic against Ps. aeruginosa. A three-drug combination of neomycin/bacitracin/polymyxin B had FIC values of < 1 for all organisms, indicating synergy and substantiating the clinical role of neomycin sulfate in current topical formulations. RESULTS: Neomycin has the lowest safety profile of the drugs in this combination. A replacement agent should ideally have similar or superior synergistic capabilities with the remaining drugs and contribute to the therapeutic efficacy of the preparation. Additionally, because of the strongly synergistic tendencies displayed by the three-drug combination, it may be possible to reduce the antibiotic concentrations present in current formulations. CONCLUSION: By developing this concept, there is potential for the formulation of topical preparations to be based on a sound theoretical and clinical rationale.

Bacitracin↗
PubMed1970

Elective induction of labour: a controlled study.

The objective of this paper is to assess the safety of elective induction, particularly with regard to fetal hazards. Emphasis is placed on the necessity of using a control group in order to obtain a meaningful comparison with patients whose labour commenced spontaneously. Two hundred and thirteen consecutive cases of electively induced labour are compared with 213 consecutive controls. The two groups are scrutinized with regard to their similarity in all respects save that of induction. Their performance in labour is documented. The perinatal and maternal complications are presented and discussed. No evidence of added risk for induced patients is found in this series.

Adult↗
PubMedDate not supplied

Recommendations for optimizing an infection control practitioner's effectiveness in an ambulatory care setting.

As the trend toward ambulatory care increases, the infection control practitioner's (ICP) role and responsibilities in this setting are continually expanding. Implementation of mandated governmental regulations, such as the Occupational Safety and Health Administration (OSHA) Bloodborne Pathogen Standard, has markedly increased the ICP's workload. Analysis reveals that much of the ICP's time is being spent on clerical tasks that encompass a variety of nonclinical functions. Clerical support would allow the ICP to use his or her time to implement essential components of an infection control program in the most cost-effective manner.

Ambulatory Care Facilities↗