Biomedical subjects
Publications and source records attributed to J H Olving.
We have used a cDNA probe for human complement component 9 (C9), which detects three DNA polymorphisms, to analyse the inheritance of C9 in families informative for C6 protein variants. We found that these genes are closely linked with a lod score of 9.28 at recombination fraction 0.00. There is not indication of allelic association.
The study comprised male citizens of Oslo, aged 30-64 years, who died outside hospital and were autopsied at the Institute of Forensic Medicine, Rikshopsitalet, from 1984 to 1988. Of the 636 cases, 195 (30.7%) were classified as alcoholics and 441 as non-alcoholics. The cause of death remained unknown after autopsy and toxicological analyses in 17.4% of the alcoholics and in 5.4% of the non-alcoholics. Suicide by other methods than medicamental poisoning was 6-7 times more frequent among non-alcoholics than among alcoholics, while death from poisoning was definitely more common among alcoholics. The frequency of lethal accidents other than intoxications was similar in both cases. Coronary heart disease was the cause of 72.7% of the natural deaths among the non-alcoholics. Among the alcoholics, however, infections (24.3%) and alcohol-related disorders (15.9%) caused nearly as many deaths as coronary heart disease (25.3%). There was a high rate of blood-alcohol concentration (greater than or equal to 0.5%) in men who died from accidents, suicides and homicides, irrespective of whether they were alcoholics or not. The findings give evidence that alcohol has a strong impact on the mortality statistics for Norwegian middle-aged men.
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An experimental study on rabbits was undertaken to evaluate the effect of the tracheostomy technique on the development of tracheal stenosis. An operation model was made for the purpose of excluding all pathogenetic factors in the formation of tracheal stenosis, except the surgical procedure. Five different operative methods were used: A. vertical incision, B. anterior wall flap, C. H-type incision, D. window-type excision, and E. subperichondreal enucleation of cartilage. The "cannulation" period was 7 days, and the animals were kept alive for another 140 days before postmortem examinations were undertaken. Minor macroscopical and microscopical changes were noted. The pathological findings were somewhat more pronounced in groups B, C and E than in the other groups. The tracheostomy technique apparently plays a minor role in the formation of tracheal stenosis.
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Typing of the sixth component of complement (C6) was performed on serum samples from 167 unrelated Norwegian Lapps. The following allele frequencies were found: C6A, 0.53 and C6B, 0.47. The phenotype distribution was in good agreement with Hardy-Weinberg expectations. Variant phenotypes were not observed. A preliminary material of 1,623 non-Lappish Norwegians showed the following allele frequencies: C6A, 0.59 and C6B, 0.41. The difference in allele distribution between the two materials was statistically significant.
Linkage relations between the C6 and 33 other genetic marker loci have been analyzed in Norwegian pedigrees, including 114 matings with 388 informative children, by use of the MOSM computer program. No suggestion of linkage was found. Very close or close linkage (theta less than 0.06) has been ruled out for males between C6 and the following 19 marker loci: GPT, HLA + Bf, Rh, C3, Hp, PGM3, Km, Gm, Fy, Gc, ABO Jk, GLO1, K, MNSs, PTC, ACP1, PGM1, and Pi. For several of the relations even loose-linkage is unlikely.
A brief review of cranio-facial fibro-osseous lesions is given and 15 cases of fibrous dysplasia and ossifying fibroma are presented with clinical, radiological and histomorphological findings. After histological verification of the fibro-osseous nature of the process, 5 lesions were diagnosed on an radiological basis as fibrous dysplasia exhibiting diffuse (blending) margins, while the diagnosis of ossifying fibroma was given to 10 radiologically sharply-demarcated lesions. Both groups of lesions presented the same histomorphological features, although to a varying degree. Spheroidal calcifications, however, were identified in ossifying fibroma only, but not in all of these. Thus, the differential diagnosis of fibrous dysplasia versus ossifying fibroma rests on a radiological criterion after the histopathologist has verified the fibro-osseous nature of a lesion. The observation times varied from 1 1/2 to 34 years. Two cases of fibrous dysplasia and 3 cases of ossifying fibroma recurred. In one case of fibrous dysplasia an osteosarcoma developed 33 years after irradiation. Pain was a common symptom in the present material.
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C6 typing was performed in a family material by two different techniques: serum or plasma samples were subjected either to high-voltage agarose gel electrophoresis or to isoelectric focusing in polyacrylamide gel slabs. Proteins with C6 activity were then visualized by a specific, hemolytic assay. In 81 unrelated adults within the family material the following allele frequencies were found: C6A:0.61 and C6B:0.39. Linkage studies exclude linkage between C6 and HLA region marker loci, and also between C6 and another chromosome 6 marker locus PGM3.