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J Hübner

Publications and source records attributed to J Hübner.

At least 37 records · Page 2Linked to original sources

[The importance of the microprecipitation reaction in agar in the diagnosis of toxoplasmosis].

The authors examined 56 pigs by an isolation experiment for Toxoplasma gondii and simultaneously by the Sabin-Feldman reaction, microprecipitation reaction in agar (MPA), quantitative microprecipitation reaction in agar (MPAK), reaction with complement-fixing and indirect haemagglutination reaction with toxoplasma antigen. In nine pigs with positive isolation finding, the Sabin-Feldman reaction and quantitative microprecipitation reaction in agar was positive in eight cases. In cases of negative Sabin-Feldman reaction, the quantitative microprecipitation reaction was always negative. The quantitative microprecipitation reaction in agar is therefore a suitable indicator of the success of isolation experiments. For a simplicity of the test it is suitable for immunological diagnostics of toxoplasmosis in pigs in common practice.

Animals↗

[Striated lupus erythematosus following the Blaschko lines].

We describe a striated cutaneous lupus erythematosus following the lines of Blaschko. These are lines along which cell populations having a common stem cell spread. If this stem cell is genetically altered, the lines of Blaschko are made visible by linear skin diseases. In the case of striated lupus erythematosus described here this genetic alteration remained unnoticed until, due to the influence of possible injury, the affected cells became antigenic, provoking a localized autoimmune disease.

Biopsy↗

[Larval toxocariasis in a 40-year-old man with detection of larvae in a liver biopsy].

A 40-year old cattle feeder has been suffering from indigestion, leukocytosis and conspicuous eosinophilia for about 4 years. A sample of liver tissue was taken during gastrectomy and multiple allergic granulomas rich in eosinophilia leukocytes were found. Toxocara canis was identified in serial sections outside the granulomas. Larval toxocariasis was confirmed by a high level of specific antibodies which failed to decrease even after administration of Mintezol antithelmintic. In this country, there was serological proof of 287 cases of larval toxocariasis till July 1982. This is the first finding of Toxocara larvae in liver biopsy published in Czechoslovak literature. Another finding of liver Toxocara larvae was made in a 86-year old woman included among 4 cases of allergic granulomatosis of the liver published before.

Adult↗

Contribution to the knowledge of the reliability of some serological tests in detecting spontaneous bovine cysticercosis.

A total of 110 bulls coming from a farm where cysticercosis repeatedly occurred were examined by means of indirect haemagglutination (IHA), microprecipitation in agar (MPA), and indirect immunofluorescence reaction (IFR). Cysticercosis (isolated cysts with live or dead cysticerci) was detected in 29 of them at post-mortem examination. Among the 29 infected animals, positive reactions were obtained in 37.9% with IHA, 51.7% with MPA and 10.3% with IFR. The remaining animals were negative. Of 82 animals giving negative reactions with IHA, 22% harboured cysticerci at post-mortem examination. Of 70 animals negative with MPA, 20% were infected with cysticerci and of 71 animals negative with IFR, 29.5% were positive at the slaughter. Consequently, none of the used serological methods is reliable for the detection of a very light spontaneous infection.

Animals↗

[Organ reaction and antibody response in experimental bovine cysticercosis].

Antibody response in the course of experimental invasion of calves with the eggs of tapeworm (Taenia saginata) was studied with the use of indirect haemagglutination reaction and microprecipitation in relation to tissue reaction. Indirect haemagglutination was positive for the first time the 21st day, microprecipitation between the 16th and 21st day, at the time of acute pathological changes induced by the developing larvae. In the period of the morphological rest of cysts, beginning about the 50th day p.i., the level of titres stabilized in the haemagglutination reaction which remained positive throughout the time of serological study (250 days p.i.). Precipitation was detectable in our set the 70th and 85th day after invasion at the latest.

Animals↗

Larva migrans lentis.

A unique case of a larva migrans lentis in a 33-year-old female is presented. The intraocular larval (obviously Toxocara) affection was characterized in the first phase by the development of a peripheral chorioretinal granuloma and in the second phase by the finding of larva migrans in the lens. The lively larva appeared several times in the pupillary area under the anterior and posterior lens capsule and survived an intensive treatment by Mintezol.

Adult↗

[Evaluation of some epidemiological factors in human toxoplasmosis].

The authors compared a group of 197 women with different ascertained forms of toxoplasmosis and a group of 72 women with negative serological reactions on toxoplasmosis and hereby they revealed that the consumption of insufficiently heated meats as well as the contact with animals (rearing and digestion embowelling or drawing of coat) play an eminent part. As test animals above all cats were used. The results of the two groups were compared and a significant difference between them was ascertained for the consumption of the insufficiently heated meat as well as for the contact with the animals. The women with toxoplasmosis more frequently consumed insufficiently heated meat and had more contact with cats and other animals.

Adult↗