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Biomedical subjects

J Hallak

Publications and source records attributed to J Hallak.

20 records · Page 2Linked to original sources

Reflections on retinoscopy.

It has often been stated that static retinoscopy findings are not affected by the examiner's refractive correction. To what extent this statement remains true is the subject of this statement remains true is the subject of this paper. The basic principle of retinoscopy is the Foucault test. In this test, a knife edge placed on the principal axis of an optical system (S) intercepts a bundle of rays coming out of (S). Depending on the position of the knife edge, various distributions of light and shadow can be observed on the anterior surface of (S). In retinoscopy it is either the entrance pupil of the examiner or the peephole of the instrument that plays the role of the knife edge. Therefore, any change in conditions that bring about a change in the "knife-edge-pupil" position will result in errors in static retinoscopy. Nevertheless, calculations show that these errors do not become significant under usual clinical conditions of static retinoscopy.

Evaluation Studies as Topic↗

Cryopreservation of human spermatozoa: comparison of TEST-yolk buffer and glycerol.

OBJECTIVE: Comparison of human sperm motility, morphology, and sperm membrane integrity using two cryoprotective media, Test-yolk buffer with glycerol and glycerol alone. METHODS: Semen samples from 10 healthy donors were divided and frozen with either glycerol or a combination of glycerol and TEST-yolk buffer. Semen characteristics were evaluated before freezing and after thawing. Motility was measured at 0, 60, 120, and 180 minutes post-thaw following removal of the cryoprotectant (post-wash). RESULTS: Percentage of motile sperm decreased significantly compared to prefreeze values in both groups. Post-thaw motility following removal of the freezing media was higher in specimens that were frozen in TEST-yolk buffer compared to those frozen in glycerol at 0 minutes (P = 0.004). Similarly, specimens cryopreserved in TEST-yolk buffer had higher sperm motility compared to aliquots that were frozen in glycerol at 180 minutes (P = 0.013). The percentage of normal sperm forms was significantly higher post-wash and post-thaw in specimens that were cryopreserved in TEST-yolk buffer (P = 0.04). CONCLUSIONS: Sperm cryopreserved in TEST-yolk buffer had significantly better motility, morphology, and sperm membrane integrity than sperm preserved in glycerol alone.

Buffers↗