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Biomedical subjects

J Hanus

Publications and source records attributed to J Hanus.

At least 37 records · Page 2Linked to original sources
PubMed1980

Two rural hospitals' projects apply swing bed concept successfully.

Alternating beds between acute and long term care may save rural hospitals from unnecessary closing and alter the balance of services to meet demand. The swing bed concept--subject of two experimental programs in Utah, Texas, South Dakota, and Iowa--may be applicable nationwide.

Beds↗
PubMed1978

Hydrogen evolution and uptake by nodules of soybeans inoculated with different strains of Rhizobium japonicum.

Hydrogen evolved by nitrogenase may be recycled by a hydrogenase present in some legume nodules. Anoka and Portage cultivars of soybeans were inoculated with each of 8 and 24 strains, respectively, of Rhizobium japonicum and surveyed for H2 evolution and C2H2 reduction rates nodule weight, and plant dry weight. Six of the strains (3Ilb 110, USDA 122, USDA 136, 3Ilb 6, 3Ilb 142, and 3Ilb 143) which exhibited no H2 evolution in air were shown to take up H2. The relative efficiencies of nitrogenase energy utilization based on C2H2 reduction rates of nodules relative efficiences of nitrogenase energy utilization based on C2H2 reduction rates of nodules ranged from 0.96 to 1.0 for the six strains. Nodules formed by strain WA 5099-1-1 evolved small amounts of H2 in air and had a relative efficiency of 0.92. Nodules formed by the remaining 25 strains had relative efficiencies ranging from 0.41 to 0.80. A H2-evolving (3Ilb 123) and non-H2-evolving (3Ilb 143) strain were tested on seven soybean cultivars to determine the effect on the expression of hydrogenase. Nodules formed by strain 3Ilb 143 exhibited an efficiency of 1.0 on the following cultivars: Amsoy 71, Anoka, Bonus, Clark 63, Kent, Peking, and Portage. Relative efficiencies from 0.63 to 0.77 were determined for the five cultivars nodulated by strain 3Ilb 123. From the experiments with these cultivars, the capacity to recycle H2 produced from the nitrogenase system appears to be determined by the R. japonicum strain.

Hydrogen↗
PubMed1977

Use of amylum derivatives for isolation of amylolytic enzymes.

In a leading article literature is reviewed concerning isolation of amylolytic enzymes by adsorption on differently modified starches, resp. on other adsorbents. DEAE amylum, DEAHP amylum and DEAE-Sephadex A 25 were found to be most suitable adsorbents. The other adsorbents examined did not reach claimed parameters.

Adsorption↗
PubMed1977

Isolation of amylolytic system of Aspergillus oryzae on DEAE amylum.

Studying isolation technique with DEAE amylum the method was found to be suitable for isolation of alpha-amylase. After determination of optimal conditions sorption curves were illustrated, from which the amount of DEAE amylum needed for 100% enzyme sorption from solutions of various activity and origin can be calculated. Preparation obtained shows high temperature stability and can be used in food industry and agriculture or after its elution in pharmacy. At the end of the paper laboratory isolation technique is described.

Adsorption↗
PubMed1977

Isolation of amylolytic system of Aspergillus oryzae by sorption on DEAHP amylum.

Conditions of effective sorption of amylolytic enzyme from a solution or from fermentative liquid on DEAHP amylum were studied. Isolating action is in a direct dependence on the relation between activity and amount of DEAHP amylum, the curve of this dependence was illustrated. The enzyme can be released by elution or adsorbate can be used in a pulverised from. In the conclusion of the work laboratory isolation technique is described.

Adsorption↗