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J Hasegawa

Publications and source records attributed to J Hasegawa.

At least 19 recordsLinked to original sources
PubMed1992

Specific reduction of delta-opioid receptor binding in transfected NG108-15 cells.

We have recently identified and sequenced the cDNA for an opioid-binding protein with homologies to cell adhesion molecules (OBCAM) (Schofield, P. R., McFarlard, K. C., Hayflick, J. S., Wilcox, J. N., Cho, T. M., Roy, S., Lee, N. M., Loh, H. H., and Seeburg, P. H. (1989) EMBO J. 8, 489-495). Several lines of evidence using antibodies suggest that OBCAM may play a functional role in NG108-15 neuroblastoma x glioma cells, a useful model system that contains a homogeneous population of delta-opioid receptors. A logical extension of this research is to further test this hypothesis. As part of this study, NG108-15 cells were stably transfected with either sense or antisense sequences of a portion of pROM, the rat cDNA for OBCAM. [3H] Diprenorphine binding was greatly reduced in antisense-transfected cells relative to non-transfected cells. Binding to alpha 2-adrenergic, muscarinic, and insulin receptors was unaffected. These results further support the notion that OBCAM or its analogue is part (or a subunit) of an opioid receptor. Furthermore, our observation of an apparently specific reduction in opioid binding in these transfected cells suggests that they may provide a novel genetic approach for studying regulation of the opioid receptor in this defined cell line.

Amino Acid Sequence↗
PubMed1992

Postoperative death of a patient in apparent remission of malignant lymphoma after dissection for squamous cell carcinoma.

A 67-year-old man in apparent remission of malignant lymphoma had squamous cell carcinoma of the left buccal mucosa. The patient underwent partial resection of the mandible, including the excision of the tumor. During the fourth postoperative night the patient suddenly became febrile and had a spiking fever for the next 5 days. His general condition deteriorated afterward, and acute aggravation of malignant lymphoma was suspected. On day 16 disseminated intravascular coagulation was indicated by a decreased platelet count of 3.8 x 10(4), a tendency toward bleeding, and multiple organ failure. The patient died 18 days postoperatively. We alert anesthesiologists and surgeons that surgically treating patients with malignant lymphoma who are receiving immunosuppressive drugs is precarious even though their disease is considered to be in apparent remission.

Aged↗
PubMed1992

Effect of loading conditions on bi-axial flexure strength of dental cements.

A ball-on-three-ball bi-axial flexure test was used to evaluate the flexure strengths of five kinds of dental cements (zinc phosphate, polycarboxylate, glass ionomer, silicate, and zinc oxide-eugenol cements). The bi-axial flexure strengths of zinc phosphate cement discs of variable dimensions were also measured to determine the size effects of test specimens. Finite element stress analyses were performed on disc specimens which were subjected to both a concentrated load and a uniformly distributed load. The observed values were compared with the theoretical value calculated by the finite element method. A ball-on-three-ball bi-axial flexure test could not be used for thicker specimens, since the fracture origin of these specimens is more likely to occur on the loaded side.

Dental Cements↗
PubMed1992

Malignant progression of a mouse fibrosarcoma by host cells reactive to a foreign body (gelatin sponge).

The QR regressor tumour (QR-32), a fibrosarcoma which is unable to grow progressively in normal syngeneic C57BL/6 mice, was able to grow progressively in 13 out of 22 mice (59%) when it was subcutaneously coimplanted with gelatin sponge. We established four culture tumour lines from the resultant tumours (QRsP tumour lines). These QRsP tumour lines were able to grow progressively in mice even in the absence of gelatin sponge. The ability of QRsP tumour cells to colonise the lungs after intravenous injection and to produce high amounts of prostaglandin E2 (PGE2) during in vitro cell culture was much greater than that of parent QR-32 cells. These biological characteristics of QR-32 cells and QRsP tumour cells were found to be stable for at least 6 months when they were maintained in culture. We also observed that QR-32 cells were able to grow progressively in five out of 12 (42%) mice after coimplantation with plastic non-adherent peritoneal cells obtained from mice which had been intraperitoneally implanted with gelatin sponge. These host cells reactive to gelatin sponge increased the production of high amounts of PGE2 by QR-32 cells during 48 h coculture. Preliminary in vitro studies implicated the involvement of hydrogen peroxide and hydroxyl radical as some of the factors necessary to induce QR-32 cells to produce high amounts of PGE2 and to accelerate tumour progression.

Animals↗
PubMed1992

Non-invasive determination of heart rate in newly hatched chicks.

1. Using a flexible piezoelectric probe, we detected non-invasively the cardiogenic precordial movements of domestic fowl chicks and measured heart rate (HR) daily over the first week after hatching (day 0) for comparison with previously measured embryonic HR. 2. HR of the hatchlings was much more variable than embryonic HR. The mean HR at a given age also varied among the 5 chicks measured. 3. The overall mean HR was 280 +/- 20 (SD, n = 5) bpm on day 0. This value was similar to the prepipping HR and lower than that of externally pipped embryos. It increased to 342 +/- 39 bpm 3 days later.

Animals↗
PubMed1992

Phase transformation of octacalcium phosphate in vivo and in vitro.

Octacalcium phosphate (OCP) was implanted in both thigh muscle pouches of ddY mice for 3 weeks, and was soaked in a simulated body fluid (SBF) and 1% NaCl solution maintained at 37 degrees C for 1 day, 1 week and 3 weeks. After implantation or soaking, the specimens were characterized by scanning electron microscopic analyses, X-ray diffractometry, and infrared absorption spectroscopy. X-ray diffraction study showed that OCP had been completely transformed to apatite with low crystallinity such as the mouse's femur after a 3-week implantation in the mouse body, and an apatic crystal was formed after soaking in the NaCl solution. The IR absorption spectra revealed that CO3 was incorporated in the apatite formed from OCP in both the mouse body and the NaCl solution, while OCP changed little after soaking in the SBF. It seems that the magnesium ion retards the transformation of OCP to apatite.

Animals↗
PubMed1992

Semi-quantitative analysis of early microleakage around amalgam restorations by fluorescent spectrum method: a laboratory study.

Rhodamine B, a fluorescent substance, was used as a tracer to investigate in vitro early microleakage from around amalgam restorations in machinable mica glass-ceramic after thermal stress. Five types of amalgam, i.e., low-copper spherical, low-copper lathe-cut, high-copper admixture, high-copper lathe-cut, and high-copper spherical, were examined in the present study. The results indicated that early microleakage from alloys of lathe-cut particles was lower than that from alloys of spherical particles in both low-copper and high-copper amalgam restorations. A high-copper amalgam with a mixture of lathe-cut and spherical particles tended to exhibit the lowest early microleakage.

Ceramics↗
PubMed1992

[Implantation of self-contained non-inflatable penile prosthesis in patients with organic impotence].

Between April, 1988 and August, 1990, the OmniPhase penile prosthesis, a non-inflatable self-contained penile prosthesis, was implanted in 34 patients, aged from 37 years to 79 years, averaging 54.2 years. The etiologies of the impotence were radical surgery for bladder cancer or rectal cancer in 17 patients, diabetes mellitus in 7 patients, vascular abnormality in 3 patients, spinal injury in 2 patients, penile disorders in 2 patients and others in 3 patients. Penile prosthesis was implanted by subcoronal incision under spinal or general anesthesia. Clinical results were evaluated 12 weeks after surgery. Thirty-two patients (94.1%) could have intercourse postoperatively. Eighteen patients (52.9%) were completely satisfied and 14 patients (41.2%) were satisfied, whereas one patient (2.9%) had no improvement and another patient (2.9%) deteriorated. There was no serious complication. However, prosthesis was explanted because of skin erosion in one patient. Pain, which lasted for more than 10 days, was seen in 3 patients (8.8%), penile edema in 11 patients (32.4%), and acute epididymitis in one patient. The obtained results showed that implantation of OmniPhase penile prosthesis is a safe and useful procedure for treatment of organic impotence.

Adult↗
PubMed1992

[Cardiac and plasma catecholamine response to dynamic exercise in hyperthyroidism].

To investigate cardiac and sympathoadrenal responses to dynamic exercise, heart rate, systolic blood pressure, serial plasma norepinephrine (NE) and epinephrine (E) concentrations during multistage treadmill exercise were measured in 24 hyperthyroid patients (mean age; 42 +/- 16) and 24 age-sex matched control subjects. Eleven patients were re-examined in the euthyroid state after antithyroid therapy. Exercise duration was shorter in patient with hyperthyroidism. Also, the heart rates and systolic blood pressures at rest and in the early stage of exercise were significantly higher in hyperthyroidism. NE at rest (normal vs hyperthyroid: 124 +/- 10 vs 80 +/- 7 pg/ml, p < 0.01) and NE at peak exercise (475 +/- 38 vs 310 +/- 38 pg/ml, p < 0.01) were lower in hyperthyroidism. E at rest (22 +/- 2 vs 29 +/- 4 pg/ml, n.s.) did not differ, however, E during the first stage of exercise (30 +/- 3 vs 69 +/- 12 pg/ml, p < 0.01) was higher in hyperthyroidism. Re-examination for the euthyroid state revealed the decreases in the heart rates and systolic blood pressures at rest and in the early stage of exercise, and the normalization of the NE and E response. Thus, patients with hyperthyroidism was in the hyperdynamic cardiac state at rest and during dynamic exercise, which was accounted for by decreased sympathetic nervous activity and increased adrenomedullary responses. These modifications of sympathoadrenal response seemed reversible when patients were controlled by antithyroid therapy.

Adolescent↗
PubMed1992

[Prostaglandin E1 infusion fails to inhibit the increase of serum granulocyte elastase and myeloperoxidase and the decrease of plasma angiotensin converting enzyme in patients undergoing open-heart surgery].

We studied whether prostaglandin E1 (PGE1) could inhibit the increase of serum granulocyte elastase (GEL) and myeloperoxidase (MPO), and the decrease of plasma angiotensin converting enzyme (ACE) induced by oxygenator in 19 patients undergoing open-heart surgery. The patients were randomly allocated into 2 groups: one group (PGE1 group, n = 9) received a continuous infusion of PGE1 at a rate of 30 ng.kg-1.min-1 during cardiopulmonary bypass (CPB), and the other group (control group, n = 10) received saline infusion. GEL, MPO and ACE were measured serially at 8 points: before induction of anesthesia (as baseline), immediately before initiation of CPB, 10 min after initiation, 60 min after initiation, immediately after the end of CPB, 60 min after CPB, 120 min after CPB, and on the first postoperative day. Serum levels of GEL and MPO during 120 min after the end of CPB in both groups increased significantly compared with the baseline values. There was no significant difference between the two groups. Plasma levels of ACE in both groups decreased significantly immediately after the end of CPB compared with values taken 10 min after the initiation of CPB. There was no significant difference between the groups. We conclude that the infusion of PGE1 30 ng.kg-1.min-1 failed to inhibit the increase of GEL as well as MPO, and the decrease of ACE.

Aged↗
PubMed1992

[Late results of coronary artery bypass grafting with the internal thoracic artery].

Between November 1981 and December 1991, 755 patients underwent isolated coronary artery bypass grafting. We compared postoperative events, graft patency and survival rates of the patients who received at least one internal thoracic artery graft (n = 517) with those of the patients who had only saphenous vein bypass grafts (n = 238). No significant difference was found between the 2 groups in patient's age at the operation, female ratio and the incidence of emergency operation. The operative mortality was not significantly different in either group. Graft patency was better for internal thoracic artery grafts than for SVG (ITA grafts 97.1%, SVG 85.6% p less than 0.0001). Graft-LAD patency rate was also better for ITA grafts than for SVG (ITA grafts 97.7%, SVG 86.8%, p less than 0.0001). The incidence of late cardiac events (late cardiac death, reoperation, myocardial infarction) is lower in patients with ITA. Furthermore, patients who received ITA grafts had a better survival rate at 8 years (91.1% versus 85.3%, p = 0.048) than those who had vein bypass grafts alone. We conclude that the use of ITA grafts enhances the long-term benefits including long-term survival rate. This is the first observation in Japanese population.

Adult↗
PubMed1992

[The epidemiology and clinical features of anaphylactic and anaphylactoid reactions in the perioperative period in Japan].

In an attempt to review the Japanese epidemiology of the anaphylactic and/or anaphylactoid reactions in the perioperative period, we investigated 105 cases with clinical features of anaphylactic and/or anaphylactoid reaction, which are reported in the Japanese anesthesiology-related journals from 1952 to 1990. Eighty-nine percent of the cases were reported during the last decade. There were 66 males and 34 females. The majority of the patients were below 60 years of age, and most of the patients were in their teens and fifties. Ninety percent of the patients had no past history of drug-induced allergy nor tendencies of atopy. Ninety-four percent of the patients recovered completely without any sequelae, and 0.95 percent of them recovered with serious complications. Deaths occurred in 4.67% of the patients. Most frequent clinical signs were cardiovascular (91.4%) and cutaneous (84.8%) manifestations. Respiratory, signs appeared in 41% of the patients. Patients are frequently unconscious and covered with drapes, and early signs and symptoms of anaphylaxis can be masked. In 25% of the patients cardiovascular collapse, including cardiac arrest and undetected blood pressure, appeared as the first noticeable sign. Causative drugs were confirmed immunologically in 5.7% of the patients. In other cases, causative drugs were presumed based on clinical course. Causative drugs and presumed causative drugs were varied, including blood and blood products (24 cases), intravenous anesthetics (19 cases), local anesthetics (15 cases), and muscle relaxants (9 cases), which were used generally in the perioperative period.

Adolescent↗
PubMed1992

[Evaluation of efficacy of a disposable monitor for surface temperature (PROCHECKER) compared with thermography].

We evaluated efficacy of a disposable monitor for surface temperature (PROCHECKER) compared with thermography. Changes in surface temperature after epidural block with mepivacaine 1% were measured using both PROCHECKER and thermography. Measured areas were regions of bilateral Th 4, 6, 8, 10, 12 and along the midmammary line. The surface temperatures on all areas were measured before, 15 min after and 30 min after the epidural block. Discrepancies of the temperatures measured by the two methods were found in 16.7% of all measured areas, and discrepancies in changes in the temperature were found in 6.7%. In conclusion, a disposable monitor for surface temperature (PROCHECKER) is effective and sensitive to measure changes in the temperature.

Disposable Equipment↗
PubMed1992

A quantitative study of postoperative luminal narrowing of the internal thoracic artery graft in coronary artery bypass surgery.

We used quantitative angiography to determine the postoperative diameter of the internal thoracic artery graft at the point close to the anastomosed site in 147 patients who received the graft for the left anterior descending coronary artery. We performed generalized multiple linear regression analysis (Type I quantification method) to assess the effects of the following factors on the internal thoracic artery graft diameter: age, gender, time of angiography, laterality of the internal thoracic artery used, presence of an undivided major side branch of the internal thoracic artery, presence of a saphenous vein graft having blood flow competition with an internal thoracic artery graft, presence of distal stenosis of the recipient left anterior descending coronary artery, severity of postoperative left anterior descending coronary artery stenosis, and presence of coronary risk factors. The standardized category scores for 25% left anterior descending coronary artery stenosis, 50% left anterior descending coronary artery stenosis, and presence of a saphenous vein graft having blood flow competition with an internal thoracic artery graft were -1.418, -0.767 and -0.622, respectively. Thus, the internal thoracic artery diameter was smaller in patients with well-preserved flow of the recipient coronary artery. The internal thoracic artery diameter had a particularly strong correlation with the degree of left anterior descending coronary artery stenosis (partial correlation coefficient: 0.670). The other factors seemed to have little or no correlation with the postoperative internal thoracic artery diameter. With the criterion that the internal thoracic artery diameter below 1.0 mm represents the "string sign" of internal thoracic artery graft, this phenomenon was observed in nine patients (6.1%). In all of these patients, left anterior descending coronary artery flow was well-preserved, and no ischemia was disclosed in the left anterior descending coronary artery-perfused area. These results indicate that internal thoracic artery grafts have flow adaptability responding to the flow demand of the recipient coronary artery and that the string sign of internal thoracic artery grafts is mainly an outcome of its physiologic characteristics.

Adult↗
PubMed1992

[The epidemiology and clinical features of anaphylactic and anaphylactoid reactions in the perioperative period in Japan: a survey with a questionnaire of 529 hospitals approved by Japan Society of Anesthesiology].

In an attempt to review the Japanese epidemiology of the anaphylactic and/or anaphylactoid reactions in the perioperative period, we surveyed 529 hospitals approved by Japan Society of Anesthesiology with a questionnaire about drug-induced immediate adverse reactions. Recovery rate of questionnaires was 15% (80 hospitals in 529 hospitals), and 28 cases of anaphylaxis or anaphylactoid reaction were reported. Most patients were in their teens and twenties. Male were 10, and female were 12 (unknown gender; 6). Reaction occurred during the induction of anesthesia in 28.6% of the patients, and during the maintenance of anesthesia in 67.9%. Seventy-five percent of the patients had no past history of drug-induced allergy nor tendencies of atopy. Most frequent clinical signs were cardiovascular (85.7%) and cutaneous (100%) manifestations. Respiratory signs appeared in 42.9% of the patients. Patients were frequently unconscious and covered with drapes, and there is a potentially danger of masking early signs and symptoms of anaphylaxis. In most of the patients, cardiovascular collapse appeared as the first noticeable sign. Causative drugs were confirmed only immunologically in one patient. In other cases causative drugs were presumed based on clinical courses. Anaphylaxis and anaphylactoid reaction are important causes of morbidity and mortality. The incidence in Japan was 0.01% (1:10,000) and the mortality was 4.76%.

Adolescent↗
PubMed1992

[Operation of female stress incontinence--comparison between Raz procedure and Gittes procedure].

We performed Raz procedure and Gittes procedure for female stress incontinence since October 1986. Raz procedure was performed on 19 patients between October 1986 and February 1990, and Gittes procedure was performed on 18 patients between June 1990 and May 1991. We followed up 17 patients who underwent Raz procedure and all the patients who underwent Gittes procedure at the time August 1991. Disappearance or marked improvement of incontinence was confirmed in 12 patients by Raz procedure, and 16 patients by Gittes Procedure. No serious complications were recognized. The reason why the result of Raz procedure was not sufficient was due to our immature operative technique and incorrect patient selection in our early experience. From this experience, we believe that both procedures can be very useful and minimal invasive operation for female stress incontinence.

Adult↗
PubMed1992

[Aortic valve replacement with fresh or cryopreserved aortic allograft--initial experience in Japan].

Aortic allograft valves were harvested from non-infected (bacterial or viral) cadavers within 24 hours of death with a family consent, and were sterilized by 4 degrees C antibiotic solution for 48 hours. Then, the allograft was preserved in the 4 degrees C nutrient medium (fresh; TC-199, calf serum and HEPES buffer) or in liquid nitrogen (-196 degrees C) after freezing to -80 degrees C by a programmed freezer. 10% dimethylsulfoxide (DMSO) was used for cryopreservation. Following germ-free confirmation, aortic allograft valves were implanted in 5 patients having aortic regurgitation with good results. Three fresh and two cryopreserved allograft valves were used. Although the follow-up term is very short (maximum 1 year) at the present time, the valve function is quite satisfactory, confirmed by cardiac catheterization and echocardiography. This is the first report in Japan with regard to cryopreservation of allograft valves and clinical use of fresh or cryopreserved valves. We believe that realization and progress of allograft preservation by cryo-technique and establishment of the tissue bank are important for the development of cardiovascular surgery in Japan.

Adult↗