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Biomedical subjects

J Hassel

Publications and source records attributed to J Hassel.

4 recordsLinked to original sources

Low-noise current amplifier based on mesoscopic Josephson junction.

We used the band structure of a mesoscopic Josephson junction to construct low-noise amplifiers. By taking advantage of the quantum dynamics of a Josephson junction, i.e., the interplay of interlevel transitions and the Coulomb blockade of Cooper pairs, we created transistor-like devices, Bloch oscillating transistors, with considerable current gain and high-input impedance. In these transistors, the correlated supercurrent of Cooper pairs is controlled by a small base current made up of single electrons. Our devices reached current and power gains on the order of 30 and 5, respectively. The noise temperature was estimated to be around 1 kelvin, but noise temperatures of less than 0.1 kelvin can be realistically achieved. These devices provide quantum-electronic building blocks that will be useful at low temperatures in low-noise circuit applications with an intermediate impedance level.

Journal Article↗

[Peripheral oxygen saturation during dental surgery with and without premedication].

83 adults undergoing dental surgical procedures in local anesthesia were monitored continuously with a pulse oximeter for hypoxemia. 30 patients received as premedication either a combination of a neuroleptic drug and an opiate or a benzodiazepine. There was a drop in the oxygen saturation in 80% of the patients with premedication but only in 66% of the patients without. There were statistical highly significant more periods of hypoxemia in patients with premedication compared to the others. (1548 periods versus 659 periods p less than 0.001). The kind of premedication/sedation does not influence the number of hypoxic episodes. We conclude that especially patients with premedication should only be treated by surgeons with sufficient clinical experience in coping with emergency situations.

Anesthesia, Dental↗

Comparison of batch and perfusion culture in combination with pilot-scale expanded bed purification for the production of soluble recombinant beta-secretase.

beta-Secretase is one of the prime targets for therapeutic intervention of Alzheimer's disease. For the development of a secretase inhibitor a steady supply of large quantities of a homogeneous and active recombinant beta-secretase is a prerequisite. Therefore various culture modes were investigated using HEK-293 cells stably transfected with soluble recombinant beta-secretase. The coupling of the Fc part of human IgG1 to the ectodomain of beta-secretase (residues 1-460) allowed a fast purification of the protein with rProtA expanded bed chromatography. Batch cultures of 5 to 50 L working volume run for 7 days showed reproducible cell growth and product yields of 3 mg/L purified protein. A 20 L perfusion culture was operated for 21 days, reaching a cell density of 30 x 10(6) cells/mL at a dilution rate of 2/d. The total product yield of the perfusion culture was 1.4 g of purified protein. The effect of different perfusion rates on cell growth, protein yield, and quality was investigated and compared to the results obtained in batch cultures. Protein quality was consistent as analyzed on 1D SDS-PAGE, and the final product contained both the mature and the pro form of beta-secretase. Although the cell specific protein expression was slightly reduced in perfusion culture, a substantial increase in specific activity of over 75% was achieved. Some of the increase in activity can be explained by an increase in the percentage of the mature form of the recombinant protein.

Amino Acid Sequence↗